Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5238 and GFF5239 are separated by 4 nucleotides GFF5239 and GFF5240 are separated by 35 nucleotides GFF5240 and GFF5241 overlap by 4 nucleotides GFF5241 and GFF5242 overlap by 1 nucleotides
GFF5238 - General secretion pathway protein L, at 104,202 to 105,269
GFF5238
GFF5239 - hypothetical protein, at 105,274 to 105,504
GFF5239
GFF5240 - General secretion pathway protein K, at 105,540 to 106,511
GFF5240
GFF5241 - General secretion pathway protein J, at 106,508 to 107,206
GFF5241
GFF5242 - General secretion pathway protein I, at 107,206 to 107,601
GFF5242
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-1
0
1 at 104.650 kb on + strand, within GFF5238 at 104.650 kb on + strand, within GFF5238 at 104.651 kb on - strand, within GFF5238 at 105.440 kb on + strand, within GFF5239 at 105.440 kb on + strand, within GFF5239 at 105.441 kb on - strand, within GFF5239 at 105.441 kb on - strand, within GFF5239 at 105.454 kb on - strand, within GFF5239 at 105.454 kb on - strand, within GFF5239 at 105.618 kb on + strand at 105.618 kb on + strand at 105.619 kb on - strand at 105.667 kb on + strand, within GFF5240 at 105.668 kb on - strand, within GFF5240 at 105.668 kb on - strand, within GFF5240 at 105.668 kb on - strand, within GFF5240 at 105.668 kb on - strand, within GFF5240 at 105.895 kb on + strand, within GFF5240 at 105.895 kb on + strand, within GFF5240 at 105.895 kb on + strand, within GFF5240 at 105.895 kb on + strand, within GFF5240 at 105.895 kb on + strand, within GFF5240 at 105.896 kb on - strand, within GFF5240 at 105.896 kb on - strand, within GFF5240 at 107.105 kb on + strand, within GFF5241 at 107.105 kb on + strand, within GFF5241 at 107.105 kb on + strand, within GFF5241 at 107.106 kb on - strand, within GFF5241 at 107.210 kb on + strand at 107.210 kb on + strand at 107.211 kb on - strand at 107.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 104,650 + GFF5238 0.42 -0.4 104,650 + GFF5238 0.42 +0.1 104,651 - GFF5238 0.42 -0.3 105,440 + GFF5239 0.72 -0.7 105,440 + GFF5239 0.72 -0.3 105,441 - GFF5239 0.72 -0.4 105,441 - GFF5239 0.72 -0.7 105,454 - GFF5239 0.78 -0.0 105,454 - GFF5239 0.78 +0.4 105,618 + -0.1 105,618 + -0.3 105,619 - -0.1 105,667 + GFF5240 0.13 +0.4 105,668 - GFF5240 0.13 +0.7 105,668 - GFF5240 0.13 -1.7 105,668 - GFF5240 0.13 +1.1 105,668 - GFF5240 0.13 +1.2 105,895 + GFF5240 0.37 -0.4 105,895 + GFF5240 0.37 -0.1 105,895 + GFF5240 0.37 +1.0 105,895 + GFF5240 0.37 -0.5 105,895 + GFF5240 0.37 -0.8 105,896 - GFF5240 0.37 +0.3 105,896 - GFF5240 0.37 -0.3 107,105 + GFF5241 0.85 -0.4 107,105 + GFF5241 0.85 -0.1 107,105 + GFF5241 0.85 +0.3 107,106 - GFF5241 0.86 +0.1 107,210 + -0.7 107,210 + +1.1 107,211 - -0.1 107,211 - -1.4
Or see this region's nucleotide sequence