Strain Fitness in Variovorax sp. SCN45 around GFF496

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF495 and GFF496 are separated by 153 nucleotidesGFF496 and GFF497 overlap by 4 nucleotidesGFF497 and GFF498 are separated by 127 nucleotides GFF495 - Kup system potassium uptake protein, at 538,246 to 540,090 GFF495 GFF496 - Squalene synthase (EC 2.5.1.21), at 540,244 to 541,158 GFF496 GFF497 - Phytoene synthase (EC 2.5.1.32), at 541,155 to 541,994 GFF497 GFF498 - Squalene/phytoene desaturase HopC, at 542,122 to 543,378 GFF498 Position (kb) 540 541 542Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 539.336 kb on + strand, within GFF495at 539.336 kb on + strand, within GFF495at 539.336 kb on + strand, within GFF495at 539.337 kb on - strand, within GFF495at 539.337 kb on - strand, within GFF495at 539.337 kb on - strand, within GFF495at 539.337 kb on - strand, within GFF495at 539.337 kb on - strand, within GFF495at 539.417 kb on + strand, within GFF495at 539.417 kb on + strand, within GFF495at 539.417 kb on + strand, within GFF495at 539.417 kb on + strand, within GFF495at 539.417 kb on + strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.418 kb on - strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.656 kb on + strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.657 kb on - strand, within GFF495at 539.771 kb on + strand, within GFF495at 539.772 kb on - strand, within GFF495at 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.924 kb on + strandat 539.925 kb on - strandat 539.925 kb on - strandat 539.925 kb on - strandat 539.925 kb on - strandat 539.925 kb on - strandat 539.925 kb on - strandat 539.925 kb on - strandat 540.038 kb on + strandat 540.038 kb on + strandat 540.039 kb on - strandat 540.039 kb on - strandat 540.126 kb on - strandat 540.126 kb on - strandat 540.126 kb on - strandat 540.206 kb on + strandat 540.207 kb on - strandat 540.207 kb on - strandat 540.283 kb on + strandat 540.283 kb on + strandat 540.283 kb on + strandat 540.284 kb on - strandat 540.284 kb on - strandat 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.350 kb on - strand, within GFF496at 540.431 kb on - strand, within GFF496at 540.526 kb on + strand, within GFF496at 540.527 kb on - strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.682 kb on + strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.793 kb on + strand, within GFF496at 540.793 kb on + strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.913 kb on + strand, within GFF496at 540.914 kb on - strand, within GFF496at 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.210 kb on - strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.213 kb on - strandat 541.213 kb on - strandat 541.213 kb on - strandat 541.263 kb on + strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.422 kb on + strand, within GFF497at 541.423 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.597 kb on - strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.797 kb on + strand, within GFF497at 541.797 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.873 kb on - strand, within GFF497at 541.873 kb on - strand, within GFF497at 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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539,336 + GFF495 0.59 -0.7
539,336 + GFF495 0.59 -0.5
539,336 + GFF495 0.59 +0.7
539,337 - GFF495 0.59 -1.0
539,337 - GFF495 0.59 -1.0
539,337 - GFF495 0.59 +0.2
539,337 - GFF495 0.59 -0.0
539,337 - GFF495 0.59 +0.3
539,417 + GFF495 0.63 -0.5
539,417 + GFF495 0.63 +0.1
539,417 + GFF495 0.63 +0.2
539,417 + GFF495 0.63 -0.7
539,417 + GFF495 0.63 -0.4
539,418 - GFF495 0.64 +2.0
539,418 - GFF495 0.64 -0.7
539,418 - GFF495 0.64 +0.0
539,418 - GFF495 0.64 -1.4
539,418 - GFF495 0.64 -0.8
539,418 - GFF495 0.64 +0.2
539,418 - GFF495 0.64 -0.2
539,418 - GFF495 0.64 -0.1
539,656 + GFF495 0.76 +0.2
539,656 + GFF495 0.76 -0.6
539,656 + GFF495 0.76 +1.3
539,656 + GFF495 0.76 -0.4
539,656 + GFF495 0.76 +2.1
539,656 + GFF495 0.76 +0.7
539,656 + GFF495 0.76 -0.2
539,656 + GFF495 0.76 +0.1
539,657 - GFF495 0.76 +0.1
539,657 - GFF495 0.76 -1.0
539,657 - GFF495 0.76 -1.2
539,657 - GFF495 0.76 +1.6
539,657 - GFF495 0.76 +0.3
539,657 - GFF495 0.76 +0.0
539,771 + GFF495 0.83 +2.0
539,772 - GFF495 0.83 -2.2
539,924 + +0.7
539,924 + -0.2
539,924 + -0.2
539,924 + -0.1
539,924 + -1.5
539,924 + -0.3
539,924 + -1.6
539,924 + +1.3
539,924 + +0.2
539,924 + +3.6
539,924 + +0.2
539,925 - -0.1
539,925 - +0.5
539,925 - -0.3
539,925 - -0.5
539,925 - +0.4
539,925 - -1.3
539,925 - -0.1
540,038 + -1.0
540,038 + +0.6
540,039 - +0.1
540,039 - -2.3
540,126 - -0.5
540,126 - +1.3
540,126 - -0.5
540,206 + +1.3
540,207 - -0.4
540,207 - +0.1
540,283 + -0.2
540,283 + +0.1
540,283 + +0.1
540,284 - -0.1
540,284 - -1.2
540,349 + GFF496 0.11 +1.3
540,349 + GFF496 0.11 +1.6
540,349 + GFF496 0.11 +0.8
540,349 + GFF496 0.11 +0.9
540,350 - GFF496 0.12 +0.5
540,431 - GFF496 0.20 -0.4
540,526 + GFF496 0.31 -0.6
540,527 - GFF496 0.31 -0.5
540,607 + GFF496 0.40 -1.7
540,607 + GFF496 0.40 +0.2
540,607 + GFF496 0.40 +1.8
540,607 + GFF496 0.40 +0.3
540,607 + GFF496 0.40 +0.8
540,607 + GFF496 0.40 +0.9
540,607 + GFF496 0.40 +0.3
540,608 - GFF496 0.40 +0.5
540,608 - GFF496 0.40 -0.0
540,608 - GFF496 0.40 -0.1
540,608 - GFF496 0.40 -2.5
540,608 - GFF496 0.40 +0.2
540,608 - GFF496 0.40 +0.9
540,608 - GFF496 0.40 +0.4
540,682 + GFF496 0.48 -0.4
540,683 - GFF496 0.48 -0.5
540,683 - GFF496 0.48 +0.0
540,683 - GFF496 0.48 +0.5
540,683 - GFF496 0.48 +1.1
540,793 + GFF496 0.60 +0.6
540,793 + GFF496 0.60 -0.2
540,794 - GFF496 0.60 -0.9
540,794 - GFF496 0.60 -0.1
540,794 - GFF496 0.60 -0.2
540,794 - GFF496 0.60 -1.6
540,913 + GFF496 0.73 +0.0
540,914 - GFF496 0.73 +1.0
541,170 + +0.5
541,170 + -1.3
541,170 + -0.8
541,170 + -0.3
541,170 + -0.0
541,170 + -0.6
541,170 + -0.1
541,170 + -0.2
541,171 - -2.5
541,171 - -0.4
541,171 - -1.2
541,171 - +0.7
541,171 - -1.0
541,171 - +1.3
541,171 - -1.2
541,171 - -0.8
541,171 - -1.3
541,171 - -0.8
541,171 - +0.3
541,171 - -1.4
541,171 - -1.5
541,171 - +0.3
541,171 - -1.6
541,210 - -1.5
541,212 + -1.4
541,212 + -1.6
541,212 + -0.8
541,212 + -1.2
541,213 - +2.3
541,213 - -0.9
541,213 - -1.9
541,263 + GFF497 0.13 +0.3
541,264 - GFF497 0.13 -2.0
541,264 - GFF497 0.13 -1.9
541,264 - GFF497 0.13 -0.0
541,422 + GFF497 0.32 -1.3
541,423 - GFF497 0.32 +0.2
541,492 - GFF497 0.40 -1.5
541,492 - GFF497 0.40 -2.2
541,492 - GFF497 0.40 -0.3
541,492 - GFF497 0.40 +0.5
541,597 - GFF497 0.53 -1.8
541,677 + GFF497 0.62 -0.5
541,677 + GFF497 0.62 -0.7
541,677 + GFF497 0.62 -1.3
541,677 + GFF497 0.62 -0.4
541,677 + GFF497 0.62 -0.4
541,677 - GFF497 0.62 -0.8
541,678 - GFF497 0.62 +0.3
541,678 - GFF497 0.62 -0.5
541,678 - GFF497 0.62 -0.0
541,678 - GFF497 0.62 -1.2
541,797 + GFF497 0.76 -1.2
541,797 + GFF497 0.76 -1.3
541,872 + GFF497 0.85 -1.4
541,872 + GFF497 0.85 -2.9
541,872 + GFF497 0.85 -0.4
541,872 + GFF497 0.85 +0.3
541,872 + GFF497 0.85 -1.5
541,873 - GFF497 0.85 -2.0
541,873 - GFF497 0.85 -0.2
541,991 - -1.2
541,991 - -0.0
541,991 - -2.4
541,991 - -1.2
541,991 - -0.0

Or see this region's nucleotide sequence