Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4856 and GFF4857 are separated by 24 nucleotides GFF4857 and GFF4858 are separated by 267 nucleotides GFF4858 and GFF4859 are separated by 188 nucleotides
GFF4856 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 225,430 to 227,211
GFF4856
GFF4857 - benzoate MFS transporter BenK, at 227,236 to 228,603
GFF4857
GFF4858 - Transposase, at 228,871 to 229,149
GFF4858
GFF4859 - MBL-fold metallo-hydrolase superfamily, at 229,338 to 230,387
GFF4859
Position (kb) 227
228
229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 226.260 kb on - strand, within GFF4856 at 226.260 kb on - strand, within GFF4856 at 226.278 kb on - strand, within GFF4856 at 226.382 kb on + strand, within GFF4856 at 226.382 kb on + strand, within GFF4856 at 226.514 kb on + strand, within GFF4856 at 226.515 kb on - strand, within GFF4856 at 226.652 kb on + strand, within GFF4856 at 226.653 kb on - strand, within GFF4856 at 226.653 kb on - strand, within GFF4856 at 226.669 kb on + strand, within GFF4856 at 227.069 kb on + strand at 227.070 kb on - strand at 227.070 kb on - strand at 227.070 kb on - strand at 227.074 kb on - strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.197 kb on + strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.198 kb on - strand at 227.201 kb on + strand at 227.201 kb on + strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.416 kb on + strand, within GFF4857 at 227.417 kb on - strand, within GFF4857 at 227.510 kb on + strand, within GFF4857 at 227.511 kb on - strand, within GFF4857 at 227.511 kb on - strand, within GFF4857 at 227.511 kb on - strand, within GFF4857 at 227.516 kb on + strand, within GFF4857 at 227.516 kb on + strand, within GFF4857 at 227.516 kb on + strand, within GFF4857 at 227.516 kb on + strand, within GFF4857 at 227.516 kb on + strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.517 kb on - strand, within GFF4857 at 227.570 kb on + strand, within GFF4857 at 227.570 kb on + strand, within GFF4857 at 227.606 kb on + strand, within GFF4857 at 227.606 kb on + strand, within GFF4857 at 227.606 kb on + strand, within GFF4857 at 227.606 kb on + strand, within GFF4857 at 227.606 kb on + strand, within GFF4857 at 227.607 kb on - strand, within GFF4857 at 227.607 kb on - strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.630 kb on + strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.631 kb on - strand, within GFF4857 at 227.750 kb on + strand, within GFF4857 at 227.751 kb on - strand, within GFF4857 at 227.760 kb on + strand, within GFF4857 at 227.761 kb on - strand, within GFF4857 at 227.795 kb on + strand, within GFF4857 at 227.795 kb on + strand, within GFF4857 at 227.795 kb on + strand, within GFF4857 at 227.795 kb on + strand, within GFF4857 at 227.796 kb on - strand, within GFF4857 at 227.796 kb on - strand, within GFF4857 at 227.796 kb on - strand, within GFF4857 at 227.796 kb on - strand, within GFF4857 at 227.796 kb on - strand, within GFF4857 at 227.955 kb on + strand, within GFF4857 at 227.956 kb on - strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.002 kb on + strand, within GFF4857 at 228.003 kb on - strand, within GFF4857 at 228.003 kb on - strand, within GFF4857 at 228.003 kb on - strand, within GFF4857 at 228.003 kb on - strand, within GFF4857 at 228.089 kb on + strand, within GFF4857 at 228.089 kb on + strand, within GFF4857 at 228.194 kb on + strand, within GFF4857 at 228.195 kb on - strand, within GFF4857 at 228.195 kb on - strand, within GFF4857 at 228.500 kb on + strand at 228.501 kb on - strand at 228.501 kb on - strand at 228.624 kb on - strand at 228.663 kb on + strand at 228.663 kb on + strand at 228.663 kb on + strand at 228.663 kb on + strand at 228.663 kb on + strand at 228.664 kb on - strand at 228.664 kb on - strand at 228.664 kb on - strand at 228.664 kb on - strand at 228.664 kb on - strand at 228.693 kb on + strand at 228.693 kb on + strand at 228.693 kb on + strand at 228.694 kb on - strand at 228.694 kb on - strand at 228.803 kb on + strand at 228.803 kb on + strand at 228.803 kb on + strand at 228.804 kb on - strand at 228.804 kb on - strand at 228.804 kb on - strand at 228.825 kb on + strand at 228.825 kb on + strand at 228.826 kb on - strand at 228.826 kb on - strand at 228.826 kb on - strand at 228.931 kb on + strand, within GFF4858 at 228.931 kb on + strand, within GFF4858 at 228.931 kb on + strand, within GFF4858 at 228.931 kb on + strand, within GFF4858 at 228.932 kb on - strand, within GFF4858 at 228.932 kb on - strand, within GFF4858 at 228.932 kb on - strand, within GFF4858 at 229.116 kb on + strand, within GFF4858 at 229.116 kb on + strand, within GFF4858 at 229.116 kb on + strand, within GFF4858 at 229.116 kb on + strand, within GFF4858 at 229.116 kb on + strand, within GFF4858 at 229.117 kb on - strand, within GFF4858 at 229.117 kb on - strand, within GFF4858 at 229.117 kb on - strand, within GFF4858 at 229.124 kb on + strand at 229.225 kb on + strand at 229.225 kb on + strand at 229.306 kb on + strand at 229.306 kb on + strand at 229.306 kb on + strand at 229.306 kb on + strand at 229.306 kb on + strand at 229.306 kb on + strand at 229.307 kb on - strand at 229.307 kb on - strand at 229.307 kb on - strand at 229.310 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.312 kb on + strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.313 kb on - strand at 229.314 kb on + strand at 229.314 kb on + strand at 229.315 kb on - strand at 229.332 kb on - strand at 229.332 kb on - strand at 229.332 kb on - strand at 229.332 kb on - strand at 229.408 kb on + strand at 229.408 kb on + strand at 229.409 kb on - strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.413 kb on + strand at 229.480 kb on + strand, within GFF4859 at 229.480 kb on + strand, within GFF4859 at 229.480 kb on + strand, within GFF4859 at 229.480 kb on + strand, within GFF4859 at 229.480 kb on + strand, within GFF4859 at 229.481 kb on - strand, within GFF4859 at 229.581 kb on + strand, within GFF4859 at 229.581 kb on + strand, within GFF4859 at 229.581 kb on + strand, within GFF4859 at 229.581 kb on + strand, within GFF4859 at 229.581 kb on + strand, within GFF4859 at 229.582 kb on - strand, within GFF4859 at 229.582 kb on - strand, within GFF4859 at 229.582 kb on - strand, within GFF4859 at 229.582 kb on - strand, within GFF4859 at 229.582 kb on - strand, within GFF4859
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 226,260 - GFF4856 0.47 +0.4 226,260 - GFF4856 0.47 +0.5 226,278 - GFF4856 0.48 -2.4 226,382 + GFF4856 0.53 -0.5 226,382 + GFF4856 0.53 +0.5 226,514 + GFF4856 0.61 -1.6 226,515 - GFF4856 0.61 -1.9 226,652 + GFF4856 0.69 -1.3 226,653 - GFF4856 0.69 -1.6 226,653 - GFF4856 0.69 -0.3 226,669 + GFF4856 0.70 +0.4 227,069 + -1.3 227,070 - +0.2 227,070 - -0.6 227,070 - -0.7 227,074 - +0.4 227,197 + -0.0 227,197 + +0.4 227,197 + -0.6 227,197 + -1.0 227,197 + -2.7 227,197 + +0.4 227,197 + +2.2 227,197 + -0.9 227,197 + +0.1 227,197 + +1.8 227,198 - +0.4 227,198 - -1.6 227,198 - +0.1 227,198 - +0.2 227,198 - +0.4 227,198 - +1.0 227,198 - -0.2 227,198 - -0.0 227,201 + -0.3 227,201 + +1.5 227,202 - +0.2 227,202 - -0.2 227,202 - -0.0 227,202 - -0.9 227,202 - +0.4 227,202 - -0.4 227,416 + GFF4857 0.13 -0.6 227,417 - GFF4857 0.13 +0.1 227,510 + GFF4857 0.20 +0.4 227,511 - GFF4857 0.20 +0.6 227,511 - GFF4857 0.20 -1.3 227,511 - GFF4857 0.20 +0.1 227,516 + GFF4857 0.20 -0.9 227,516 + GFF4857 0.20 -1.1 227,516 + GFF4857 0.20 -0.4 227,516 + GFF4857 0.20 +0.6 227,516 + GFF4857 0.20 -0.9 227,517 - GFF4857 0.21 -0.0 227,517 - GFF4857 0.21 +0.2 227,517 - GFF4857 0.21 +0.5 227,517 - GFF4857 0.21 -0.0 227,517 - GFF4857 0.21 -0.2 227,517 - GFF4857 0.21 +0.1 227,517 - GFF4857 0.21 +0.4 227,570 + GFF4857 0.24 -0.6 227,570 + GFF4857 0.24 -0.3 227,606 + GFF4857 0.27 -0.1 227,606 + GFF4857 0.27 +0.5 227,606 + GFF4857 0.27 -0.4 227,606 + GFF4857 0.27 +0.3 227,606 + GFF4857 0.27 +0.1 227,607 - GFF4857 0.27 -0.3 227,607 - GFF4857 0.27 +0.9 227,630 + GFF4857 0.29 -1.1 227,630 + GFF4857 0.29 -0.0 227,630 + GFF4857 0.29 -1.5 227,630 + GFF4857 0.29 +0.3 227,630 + GFF4857 0.29 +0.0 227,630 + GFF4857 0.29 -0.8 227,630 + GFF4857 0.29 -0.7 227,630 + GFF4857 0.29 -0.9 227,630 + GFF4857 0.29 +1.4 227,630 + GFF4857 0.29 +3.2 227,630 + GFF4857 0.29 -2.4 227,630 + GFF4857 0.29 -0.3 227,630 + GFF4857 0.29 +0.0 227,630 + GFF4857 0.29 -1.0 227,630 + GFF4857 0.29 +0.2 227,630 + GFF4857 0.29 +0.1 227,630 + GFF4857 0.29 -0.6 227,630 + GFF4857 0.29 +0.0 227,630 + GFF4857 0.29 -0.7 227,631 - GFF4857 0.29 -2.1 227,631 - GFF4857 0.29 -1.2 227,631 - GFF4857 0.29 -0.9 227,631 - GFF4857 0.29 -0.4 227,631 - GFF4857 0.29 -1.3 227,631 - GFF4857 0.29 -2.5 227,631 - GFF4857 0.29 +0.8 227,631 - GFF4857 0.29 +2.0 227,631 - GFF4857 0.29 +0.3 227,631 - GFF4857 0.29 -0.1 227,631 - GFF4857 0.29 +0.6 227,631 - GFF4857 0.29 -0.2 227,631 - GFF4857 0.29 +0.5 227,631 - GFF4857 0.29 +0.6 227,631 - GFF4857 0.29 +1.2 227,750 + GFF4857 0.38 -0.6 227,751 - GFF4857 0.38 -0.0 227,760 + GFF4857 0.38 -1.3 227,761 - GFF4857 0.38 +0.2 227,795 + GFF4857 0.41 +0.3 227,795 + GFF4857 0.41 +0.9 227,795 + GFF4857 0.41 -1.6 227,795 + GFF4857 0.41 -1.6 227,796 - GFF4857 0.41 -0.0 227,796 - GFF4857 0.41 +0.3 227,796 - GFF4857 0.41 +0.9 227,796 - GFF4857 0.41 -0.5 227,796 - GFF4857 0.41 -1.0 227,955 + GFF4857 0.53 -0.2 227,956 - GFF4857 0.53 -0.6 228,002 + GFF4857 0.56 -0.1 228,002 + GFF4857 0.56 -0.4 228,002 + GFF4857 0.56 +0.3 228,002 + GFF4857 0.56 +0.4 228,002 + GFF4857 0.56 -0.5 228,002 + GFF4857 0.56 +0.1 228,002 + GFF4857 0.56 +0.7 228,002 + GFF4857 0.56 +0.8 228,003 - GFF4857 0.56 -0.5 228,003 - GFF4857 0.56 -0.6 228,003 - GFF4857 0.56 -0.8 228,003 - GFF4857 0.56 -0.6 228,089 + GFF4857 0.62 -1.2 228,089 + GFF4857 0.62 +0.0 228,194 + GFF4857 0.70 -0.2 228,195 - GFF4857 0.70 -1.4 228,195 - GFF4857 0.70 +0.2 228,500 + +0.7 228,501 - -2.6 228,501 - -0.4 228,624 - +0.4 228,663 + +1.7 228,663 + +0.4 228,663 + +0.2 228,663 + -0.2 228,663 + -0.9 228,664 - -2.8 228,664 - +1.1 228,664 - -1.8 228,664 - +0.2 228,664 - -0.1 228,693 + -0.0 228,693 + -0.1 228,693 + -0.1 228,694 - -0.4 228,694 - +0.8 228,803 + -0.4 228,803 + +0.4 228,803 + -2.0 228,804 - -0.5 228,804 - -0.6 228,804 - +0.1 228,825 + +1.4 228,825 + -0.2 228,826 - -0.9 228,826 - -1.0 228,826 - -0.6 228,931 + GFF4858 0.22 -0.5 228,931 + GFF4858 0.22 +0.2 228,931 + GFF4858 0.22 -0.2 228,931 + GFF4858 0.22 +0.2 228,932 - GFF4858 0.22 +0.2 228,932 - GFF4858 0.22 -1.3 228,932 - GFF4858 0.22 +0.6 229,116 + GFF4858 0.88 -2.0 229,116 + GFF4858 0.88 -1.0 229,116 + GFF4858 0.88 -0.3 229,116 + GFF4858 0.88 -0.8 229,116 + GFF4858 0.88 -0.6 229,117 - GFF4858 0.88 -0.2 229,117 - GFF4858 0.88 +0.8 229,117 - GFF4858 0.88 -0.9 229,124 + -0.1 229,225 + -0.2 229,225 + -0.0 229,306 + -0.8 229,306 + -1.3 229,306 + +0.1 229,306 + -0.2 229,306 + -1.4 229,306 + -0.5 229,307 - -2.0 229,307 - -1.1 229,307 - -1.8 229,310 + -0.7 229,312 + +0.1 229,312 + -0.5 229,312 + -1.1 229,312 + -1.4 229,312 + +0.1 229,312 + +0.2 229,312 + -0.8 229,312 + +0.1 229,312 + +0.5 229,312 + -1.1 229,312 + -0.7 229,313 - +0.4 229,313 - -0.6 229,313 - +0.4 229,313 - -0.4 229,313 - +0.1 229,313 - -1.3 229,313 - -0.2 229,313 - -0.2 229,313 - -0.0 229,314 + +0.9 229,314 + -1.1 229,315 - +0.4 229,332 - +1.3 229,332 - -0.7 229,332 - -0.2 229,332 - +0.2 229,408 + +0.5 229,408 + -1.0 229,409 - -3.0 229,413 + -0.4 229,413 + -0.5 229,413 + -0.4 229,413 + +0.7 229,413 + +0.6 229,413 + -0.8 229,413 + -0.2 229,413 + +0.5 229,413 + -0.2 229,480 + GFF4859 0.14 +0.2 229,480 + GFF4859 0.14 +0.5 229,480 + GFF4859 0.14 -0.6 229,480 + GFF4859 0.14 +0.0 229,480 + GFF4859 0.14 -1.9 229,481 - GFF4859 0.14 -0.3 229,581 + GFF4859 0.23 +0.2 229,581 + GFF4859 0.23 -1.2 229,581 + GFF4859 0.23 -0.3 229,581 + GFF4859 0.23 +0.2 229,581 + GFF4859 0.23 +0.5 229,582 - GFF4859 0.23 -0.7 229,582 - GFF4859 0.23 -1.3 229,582 - GFF4859 0.23 -0.4 229,582 - GFF4859 0.23 -0.7 229,582 - GFF4859 0.23 -0.6
Or see this region's nucleotide sequence