Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4846 and GFF4847 are separated by 19 nucleotides GFF4847 and GFF4848 overlap by 4 nucleotides GFF4848 and GFF4849 overlap by 4 nucleotides
GFF4846 - Conjugative transfer protein TrbE, at 215,637 to 218,114
GFF4846
GFF4847 - Conjugative transfer protein TrbD, at 218,134 to 218,418
GFF4847
GFF4848 - Conjugative transfer protein TrbC, at 218,415 to 218,807
GFF4848
GFF4849 - Conjugative transfer protein TrbB, at 218,804 to 219,841
GFF4849
Position (kb) 218
219 Strain fitness (log2 ratio) -2
-1
0
1 at 217.141 kb on + strand, within GFF4846 at 217.337 kb on + strand, within GFF4846 at 217.338 kb on - strand, within GFF4846 at 217.338 kb on - strand, within GFF4846 at 217.338 kb on - strand, within GFF4846 at 217.538 kb on + strand, within GFF4846 at 217.538 kb on + strand, within GFF4846 at 217.539 kb on - strand, within GFF4846 at 217.558 kb on + strand, within GFF4846 at 217.558 kb on + strand, within GFF4846 at 217.558 kb on + strand, within GFF4846 at 217.558 kb on + strand, within GFF4846 at 217.558 kb on + strand, within GFF4846 at 217.559 kb on - strand, within GFF4846 at 217.575 kb on - strand, within GFF4846 at 217.696 kb on + strand, within GFF4846 at 217.696 kb on + strand, within GFF4846 at 217.696 kb on + strand, within GFF4846 at 217.741 kb on + strand, within GFF4846 at 217.741 kb on + strand, within GFF4846 at 217.741 kb on + strand, within GFF4846 at 217.741 kb on + strand, within GFF4846 at 217.742 kb on - strand, within GFF4846 at 217.742 kb on - strand, within GFF4846 at 217.742 kb on - strand, within GFF4846 at 217.742 kb on - strand, within GFF4846 at 217.760 kb on - strand, within GFF4846 at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.944 kb on - strand at 217.944 kb on - strand at 217.944 kb on - strand at 217.944 kb on - strand at 218.021 kb on + strand at 218.021 kb on + strand at 218.022 kb on - strand at 218.022 kb on - strand at 218.022 kb on - strand at 218.119 kb on + strand at 218.119 kb on + strand at 218.120 kb on - strand at 218.135 kb on + strand at 218.136 kb on - strand at 218.136 kb on - strand at 218.163 kb on + strand, within GFF4847 at 218.164 kb on - strand, within GFF4847 at 218.419 kb on - strand at 218.419 kb on - strand at 218.777 kb on + strand at 218.902 kb on - strand at 218.902 kb on - strand at 219.119 kb on + strand, within GFF4849 at 219.119 kb on + strand, within GFF4849 at 219.119 kb on + strand, within GFF4849 at 219.120 kb on - strand, within GFF4849 at 219.245 kb on + strand, within GFF4849 at 219.245 kb on + strand, within GFF4849 at 219.246 kb on - strand, within GFF4849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 217,141 + GFF4846 0.61 +1.0 217,337 + GFF4846 0.69 -0.2 217,338 - GFF4846 0.69 +0.2 217,338 - GFF4846 0.69 +0.5 217,338 - GFF4846 0.69 -1.8 217,538 + GFF4846 0.77 +0.4 217,538 + GFF4846 0.77 +0.8 217,539 - GFF4846 0.77 -1.0 217,558 + GFF4846 0.78 +1.4 217,558 + GFF4846 0.78 -0.1 217,558 + GFF4846 0.78 -0.4 217,558 + GFF4846 0.78 -1.8 217,558 + GFF4846 0.78 +0.3 217,559 - GFF4846 0.78 +1.4 217,575 - GFF4846 0.78 -0.2 217,696 + GFF4846 0.83 -1.6 217,696 + GFF4846 0.83 -1.1 217,696 + GFF4846 0.83 +0.1 217,741 + GFF4846 0.85 -1.2 217,741 + GFF4846 0.85 +0.3 217,741 + GFF4846 0.85 +0.2 217,741 + GFF4846 0.85 -0.0 217,742 - GFF4846 0.85 -0.6 217,742 - GFF4846 0.85 -0.0 217,742 - GFF4846 0.85 -0.2 217,742 - GFF4846 0.85 +0.8 217,760 - GFF4846 0.86 -0.9 217,943 + -0.3 217,943 + -0.6 217,943 + -0.4 217,943 + -0.4 217,943 + +0.1 217,943 + -0.0 217,944 - +0.8 217,944 - -0.1 217,944 - -1.7 217,944 - +1.0 218,021 + -0.6 218,021 + -0.8 218,022 - -1.7 218,022 - +0.2 218,022 - -0.0 218,119 + -0.8 218,119 + +0.6 218,120 - -0.0 218,135 + -0.5 218,136 - -0.3 218,136 - -1.3 218,163 + GFF4847 0.10 -0.0 218,164 - GFF4847 0.11 -1.0 218,419 - -0.3 218,419 - -1.2 218,777 + +0.1 218,902 - -0.1 218,902 - +1.4 219,119 + GFF4849 0.30 -0.1 219,119 + GFF4849 0.30 -1.0 219,119 + GFF4849 0.30 +0.8 219,120 - GFF4849 0.30 -2.0 219,245 + GFF4849 0.42 -1.0 219,245 + GFF4849 0.42 -1.0 219,246 - GFF4849 0.43 -0.0
Or see this region's nucleotide sequence