Strain Fitness in Variovorax sp. SCN45 around GFF4731

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4730 and GFF4731 are separated by 4 nucleotidesGFF4731 and GFF4732 are separated by 48 nucleotidesGFF4732 and GFF4733 are separated by 9 nucleotides GFF4730 - hypothetical protein, at 92,439 to 94,712 GFF4730 GFF4731 - Assimilatory nitrate reductase large subunit (EC 1.7.99.4), at 94,717 to 97,509 GFF4731 GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953 GFF4732 GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754 GFF4733 Position (kb) 94 95 96 97 98Strain fitness (log2 ratio) -2 -1 0 1 2at 93.787 kb on - strand, within GFF4730at 93.787 kb on - strand, within GFF4730at 93.787 kb on - strand, within GFF4730at 93.828 kb on + strand, within GFF4730at 93.828 kb on + strand, within GFF4730at 93.828 kb on + strand, within GFF4730at 93.829 kb on - strand, within GFF4730at 93.829 kb on - strand, within GFF4730at 93.909 kb on + strand, within GFF4730at 93.909 kb on + strand, within GFF4730at 94.051 kb on - strand, within GFF4730at 94.120 kb on - strand, within GFF4730at 94.152 kb on + strand, within GFF4730at 94.152 kb on + strand, within GFF4730at 94.152 kb on + strand, within GFF4730at 94.152 kb on + strand, within GFF4730at 94.153 kb on - strand, within GFF4730at 94.153 kb on - strand, within GFF4730at 94.153 kb on - strand, within GFF4730at 94.443 kb on + strand, within GFF4730at 94.444 kb on - strand, within GFF4730at 94.444 kb on - strand, within GFF4730at 94.444 kb on - strand, within GFF4730at 94.491 kb on + strandat 94.491 kb on + strandat 95.495 kb on + strand, within GFF4731at 95.495 kb on + strand, within GFF4731at 95.496 kb on - strand, within GFF4731at 95.496 kb on - strand, within GFF4731at 95.786 kb on + strand, within GFF4731at 95.786 kb on + strand, within GFF4731at 95.787 kb on - strand, within GFF4731at 95.787 kb on - strand, within GFF4731at 95.787 kb on - strand, within GFF4731at 95.844 kb on - strand, within GFF4731at 95.919 kb on - strand, within GFF4731at 95.919 kb on - strand, within GFF4731at 96.620 kb on + strand, within GFF4731at 96.621 kb on - strand, within GFF4731at 96.621 kb on - strand, within GFF4731at 96.621 kb on - strand, within GFF4731at 96.718 kb on - strand, within GFF4731at 96.718 kb on - strand, within GFF4731at 96.718 kb on - strand, within GFF4731at 96.773 kb on + strand, within GFF4731at 96.774 kb on - strand, within GFF4731at 96.774 kb on - strand, within GFF4731at 96.774 kb on - strand, within GFF4731at 96.774 kb on - strand, within GFF4731at 97.029 kb on - strand, within GFF4731at 97.067 kb on + strand, within GFF4731at 97.068 kb on - strand, within GFF4731at 97.157 kb on + strand, within GFF4731at 97.157 kb on + strand, within GFF4731at 97.157 kb on + strand, within GFF4731at 97.158 kb on - strand, within GFF4731at 97.158 kb on - strand, within GFF4731at 97.158 kb on - strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.160 kb on + strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.161 kb on - strand, within GFF4731at 97.190 kb on + strand, within GFF4731at 97.191 kb on - strand, within GFF4731at 97.481 kb on + strandat 97.560 kb on - strandat 97.964 kb on + strandat 97.964 kb on + strandat 97.965 kb on - strandat 97.965 kb on - strandat 97.965 kb on - strandat 97.965 kb on - strandat 97.982 kb on + strandat 97.983 kb on - strandat 98.258 kb on + strand, within GFF4733at 98.259 kb on - strand, within GFF4733at 98.345 kb on + strand, within GFF4733at 98.480 kb on + strand, within GFF4733at 98.481 kb on - strand, within GFF4733

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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93,787 - GFF4730 0.59 -0.5
93,787 - GFF4730 0.59 -0.6
93,787 - GFF4730 0.59 -0.0
93,828 + GFF4730 0.61 -2.0
93,828 + GFF4730 0.61 -0.5
93,828 + GFF4730 0.61 -0.1
93,829 - GFF4730 0.61 +1.3
93,829 - GFF4730 0.61 +0.4
93,909 + GFF4730 0.65 -0.5
93,909 + GFF4730 0.65 -0.8
94,051 - GFF4730 0.71 +1.2
94,120 - GFF4730 0.74 -0.2
94,152 + GFF4730 0.75 +0.7
94,152 + GFF4730 0.75 -2.6
94,152 + GFF4730 0.75 -0.7
94,152 + GFF4730 0.75 +0.2
94,153 - GFF4730 0.75 -0.0
94,153 - GFF4730 0.75 -0.6
94,153 - GFF4730 0.75 +0.4
94,443 + GFF4730 0.88 -0.0
94,444 - GFF4730 0.88 +0.6
94,444 - GFF4730 0.88 -0.6
94,444 - GFF4730 0.88 +0.2
94,491 + +0.7
94,491 + -0.2
95,495 + GFF4731 0.28 -1.0
95,495 + GFF4731 0.28 +0.6
95,496 - GFF4731 0.28 +1.7
95,496 - GFF4731 0.28 -0.6
95,786 + GFF4731 0.38 -0.5
95,786 + GFF4731 0.38 -0.5
95,787 - GFF4731 0.38 -0.5
95,787 - GFF4731 0.38 +0.0
95,787 - GFF4731 0.38 -0.1
95,844 - GFF4731 0.40 -0.5
95,919 - GFF4731 0.43 -1.0
95,919 - GFF4731 0.43 -1.2
96,620 + GFF4731 0.68 +1.1
96,621 - GFF4731 0.68 -0.4
96,621 - GFF4731 0.68 +0.2
96,621 - GFF4731 0.68 -0.1
96,718 - GFF4731 0.72 -0.9
96,718 - GFF4731 0.72 -0.6
96,718 - GFF4731 0.72 +0.1
96,773 + GFF4731 0.74 +0.8
96,774 - GFF4731 0.74 +0.1
96,774 - GFF4731 0.74 -0.9
96,774 - GFF4731 0.74 +0.4
96,774 - GFF4731 0.74 -0.2
97,029 - GFF4731 0.83 +0.5
97,067 + GFF4731 0.84 +1.6
97,068 - GFF4731 0.84 -0.4
97,157 + GFF4731 0.87 -0.6
97,157 + GFF4731 0.87 -2.0
97,157 + GFF4731 0.87 -1.0
97,158 - GFF4731 0.87 -0.2
97,158 - GFF4731 0.87 -0.8
97,158 - GFF4731 0.87 +1.0
97,160 + GFF4731 0.87 -0.1
97,160 + GFF4731 0.87 -0.2
97,160 + GFF4731 0.87 -0.2
97,160 + GFF4731 0.87 -0.6
97,160 + GFF4731 0.87 -1.8
97,160 + GFF4731 0.87 +0.2
97,161 - GFF4731 0.88 -0.4
97,161 - GFF4731 0.88 -1.4
97,161 - GFF4731 0.88 +0.9
97,161 - GFF4731 0.88 +0.6
97,161 - GFF4731 0.88 +0.5
97,161 - GFF4731 0.88 -0.5
97,190 + GFF4731 0.89 +0.6
97,191 - GFF4731 0.89 -0.3
97,481 + -1.3
97,560 - -0.6
97,964 + +1.2
97,964 + +0.3
97,965 - +1.0
97,965 - -1.0
97,965 - +0.4
97,965 - +0.6
97,982 + +0.2
97,983 - +1.8
98,258 + GFF4733 0.37 +0.2
98,259 - GFF4733 0.37 -0.3
98,345 + GFF4733 0.48 -0.4
98,480 + GFF4733 0.65 -1.2
98,481 - GFF4733 0.65 +1.1

Or see this region's nucleotide sequence