Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4591 and GFF4592 are separated by 1 nucleotides GFF4592 and GFF4593 are separated by 98 nucleotides GFF4593 and GFF4594 are separated by 153 nucleotides
GFF4591 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 231,949 to 232,617
GFF4591
GFF4592 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 232,619 to 233,377
GFF4592
GFF4593 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 233,476 to 234,372
GFF4593
GFF4594 - Aspartate ammonia-lyase (EC 4.3.1.1), at 234,526 to 235,998
GFF4594
Position (kb) 233
234
235 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 232.550 kb on + strand, within GFF4591 at 232.550 kb on + strand, within GFF4591 at 232.551 kb on - strand at 232.551 kb on - strand at 232.551 kb on - strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.948 kb on - strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.194 kb on + strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.477 kb on + strand at 233.477 kb on + strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.489 kb on + strand at 233.489 kb on + strand at 233.490 kb on - strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.536 kb on - strand at 233.603 kb on + strand, within GFF4593 at 233.604 kb on - strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.131 kb on + strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.404 kb on - strand at 234.407 kb on + strand at 234.407 kb on + strand at 234.408 kb on - strand at 234.408 kb on - strand at 234.420 kb on + strand at 234.420 kb on + strand at 234.549 kb on + strand at 234.549 kb on + strand at 234.549 kb on + strand at 234.549 kb on + strand at 234.550 kb on - strand at 234.550 kb on - strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.604 kb on + strand at 234.605 kb on - strand at 234.605 kb on - strand at 234.605 kb on - strand at 234.740 kb on + strand, within GFF4594 at 235.248 kb on - strand, within GFF4594 at 235.248 kb on - strand, within GFF4594 at 235.248 kb on - strand, within GFF4594 at 235.248 kb on - strand, within GFF4594 at 235.268 kb on + strand, within GFF4594 at 235.269 kb on - strand, within GFF4594 at 235.269 kb on - strand, within GFF4594
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 232,550 + GFF4591 0.90 -0.0 232,550 + GFF4591 0.90 -1.7 232,551 - -0.5 232,551 - -0.8 232,551 - -0.1 232,568 + -0.7 232,568 + +0.4 232,568 + +1.2 232,568 + +0.2 232,568 + +0.3 232,568 + +0.7 232,568 + +0.6 232,568 + +0.1 232,568 + -1.5 232,568 + -0.4 232,568 + -0.8 232,568 + +0.2 232,568 + +0.5 232,568 + -0.6 232,568 + -0.1 232,568 + -0.5 232,568 + -1.7 232,568 + -0.1 232,568 + -0.9 232,568 + -0.2 232,568 + -0.5 232,569 - -0.8 232,569 - -0.1 232,569 - +0.0 232,569 - -0.4 232,569 - -1.8 232,569 - +0.1 232,569 - -0.4 232,569 - +0.2 232,569 - -0.7 232,569 - -0.5 232,569 - -1.5 232,569 - -0.8 232,569 - -0.5 232,569 - -0.4 232,569 - +0.2 232,592 + -0.3 232,592 + -2.0 232,592 + +0.0 232,592 + -0.1 232,593 - +0.8 232,593 - +0.1 232,719 + GFF4592 0.13 -2.7 232,719 + GFF4592 0.13 -1.1 232,719 + GFF4592 0.13 +0.4 232,719 + GFF4592 0.13 +0.2 232,719 + GFF4592 0.13 -1.2 232,719 + GFF4592 0.13 -0.7 232,719 + GFF4592 0.13 -1.5 232,719 + GFF4592 0.13 -0.1 232,719 + GFF4592 0.13 -0.4 232,720 - GFF4592 0.13 +1.1 232,720 - GFF4592 0.13 -0.0 232,720 - GFF4592 0.13 +1.1 232,720 - GFF4592 0.13 -0.9 232,720 - GFF4592 0.13 -0.9 232,720 - GFF4592 0.13 -2.3 232,720 - GFF4592 0.13 +0.7 232,720 - GFF4592 0.13 +0.6 232,720 - GFF4592 0.13 -0.0 232,720 - GFF4592 0.13 -0.8 232,740 + GFF4592 0.16 -0.2 232,740 + GFF4592 0.16 -0.4 232,741 - GFF4592 0.16 +0.6 232,741 - GFF4592 0.16 +0.5 232,782 + GFF4592 0.21 -0.7 232,782 + GFF4592 0.21 +0.8 232,908 + GFF4592 0.38 -0.6 232,908 + GFF4592 0.38 -0.5 232,908 + GFF4592 0.38 -0.2 232,908 + GFF4592 0.38 -0.4 232,909 - GFF4592 0.38 -0.5 232,909 - GFF4592 0.38 +0.8 232,911 + GFF4592 0.38 -0.4 232,911 + GFF4592 0.38 -0.1 232,911 + GFF4592 0.38 -0.3 232,911 + GFF4592 0.38 -2.5 232,911 + GFF4592 0.38 -0.4 232,911 + GFF4592 0.38 +3.8 232,911 + GFF4592 0.38 +0.6 232,911 + GFF4592 0.38 +0.7 232,912 - GFF4592 0.39 +0.2 232,912 - GFF4592 0.39 -0.4 232,912 - GFF4592 0.39 -0.3 232,948 - GFF4592 0.43 -0.1 233,091 + GFF4592 0.62 -0.3 233,091 + GFF4592 0.62 +0.3 233,092 - GFF4592 0.62 +0.8 233,092 - GFF4592 0.62 -1.5 233,092 - GFF4592 0.62 -0.2 233,092 - GFF4592 0.62 -0.2 233,194 + GFF4592 0.76 -0.9 233,195 - GFF4592 0.76 +0.2 233,195 - GFF4592 0.76 -0.3 233,195 - GFF4592 0.76 -0.7 233,195 - GFF4592 0.76 -0.7 233,195 - GFF4592 0.76 -1.9 233,286 + GFF4592 0.88 +0.7 233,286 + GFF4592 0.88 +0.8 233,286 + GFF4592 0.88 -0.6 233,287 - GFF4592 0.88 -0.6 233,287 - GFF4592 0.88 +0.0 233,287 - GFF4592 0.88 -2.3 233,287 - GFF4592 0.88 -0.3 233,287 - GFF4592 0.88 -0.4 233,412 + -0.2 233,412 + +0.1 233,412 + -1.1 233,412 + +0.4 233,412 + -0.4 233,412 + -0.5 233,412 + -1.9 233,413 - +0.7 233,413 - +1.8 233,413 - +1.8 233,413 - -0.4 233,413 - -0.2 233,413 - +0.1 233,477 + +0.4 233,477 + -0.2 233,478 - -0.5 233,478 - -0.1 233,478 - -0.7 233,489 + +0.8 233,489 + -1.1 233,490 - +0.8 233,535 + +0.1 233,535 + +0.2 233,535 + -0.6 233,535 + -0.4 233,536 - -0.2 233,603 + GFF4593 0.14 -0.7 233,604 - GFF4593 0.14 -1.0 234,008 + GFF4593 0.59 -0.3 234,008 + GFF4593 0.59 -1.1 234,008 + GFF4593 0.59 +0.3 234,008 + GFF4593 0.59 +0.4 234,008 + GFF4593 0.59 -0.2 234,008 + GFF4593 0.59 +1.2 234,008 + GFF4593 0.59 -0.5 234,008 + GFF4593 0.59 -0.1 234,008 + GFF4593 0.59 +0.3 234,008 + GFF4593 0.59 -0.3 234,008 + GFF4593 0.59 -2.0 234,008 + GFF4593 0.59 +0.3 234,008 + GFF4593 0.59 -0.2 234,009 - GFF4593 0.59 -3.8 234,009 - GFF4593 0.59 -1.6 234,009 - GFF4593 0.59 +0.8 234,009 - GFF4593 0.59 -0.1 234,009 - GFF4593 0.59 -1.4 234,009 - GFF4593 0.59 -0.8 234,009 - GFF4593 0.59 -1.7 234,009 - GFF4593 0.59 -0.4 234,009 - GFF4593 0.59 -0.2 234,009 - GFF4593 0.59 -0.5 234,009 - GFF4593 0.59 -0.7 234,017 + GFF4593 0.60 -0.5 234,017 + GFF4593 0.60 +1.4 234,017 + GFF4593 0.60 -0.1 234,018 - GFF4593 0.60 -0.6 234,018 - GFF4593 0.60 -0.3 234,018 - GFF4593 0.60 -1.2 234,131 + GFF4593 0.73 -0.3 234,132 - GFF4593 0.73 +0.5 234,132 - GFF4593 0.73 -0.3 234,132 - GFF4593 0.73 -0.4 234,132 - GFF4593 0.73 +0.3 234,212 + GFF4593 0.82 -0.1 234,212 + GFF4593 0.82 -0.4 234,212 + GFF4593 0.82 -1.7 234,212 + GFF4593 0.82 -2.1 234,212 + GFF4593 0.82 -0.6 234,212 + GFF4593 0.82 +0.2 234,213 - GFF4593 0.82 -1.0 234,213 - GFF4593 0.82 +0.2 234,213 - GFF4593 0.82 -0.5 234,213 - GFF4593 0.82 -1.9 234,213 - GFF4593 0.82 -0.3 234,213 - GFF4593 0.82 -2.3 234,213 - GFF4593 0.82 -0.5 234,213 - GFF4593 0.82 -0.5 234,213 - GFF4593 0.82 -0.4 234,233 + GFF4593 0.84 -0.3 234,233 + GFF4593 0.84 -1.2 234,233 + GFF4593 0.84 -0.4 234,234 - GFF4593 0.85 -1.8 234,234 - GFF4593 0.85 -0.5 234,234 - GFF4593 0.85 -0.5 234,234 - GFF4593 0.85 +1.5 234,404 - +0.4 234,407 + +0.2 234,407 + +0.9 234,408 - -0.4 234,408 - +0.6 234,420 + +0.7 234,420 + +0.3 234,549 + +0.1 234,549 + -0.7 234,549 + -0.4 234,549 + -0.1 234,550 - -0.5 234,550 - +0.2 234,604 + -0.4 234,604 + -0.3 234,604 + -0.9 234,604 + -0.5 234,604 + -1.7 234,604 + -0.6 234,604 + -0.1 234,605 - -0.7 234,605 - +0.6 234,605 - -0.3 234,740 + GFF4594 0.15 -0.3 235,248 - GFF4594 0.49 -0.1 235,248 - GFF4594 0.49 -1.0 235,248 - GFF4594 0.49 -0.3 235,248 - GFF4594 0.49 -0.2 235,268 + GFF4594 0.50 +0.5 235,269 - GFF4594 0.50 -0.7 235,269 - GFF4594 0.50 +0.4
Or see this region's nucleotide sequence