Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4293 and GFF4294 overlap by 4 nucleotides GFF4294 and GFF4295 overlap by 4 nucleotides GFF4295 and GFF4296 overlap by 4 nucleotides GFF4296 and GFF4297 are separated by 22 nucleotides GFF4297 and GFF4298 are separated by 42 nucleotides
GFF4293 - 4-hydroxybutyrate coenzyme A transferase, at 212,672 to 213,916
GFF4293
GFF4294 - hypothetical protein, at 213,913 to 214,317
GFF4294
GFF4295 - no description, at 214,314 to 214,775
GFF4295
GFF4296 - hypothetical protein, at 214,772 to 216,310
GFF4296
GFF4297 - 2-ketocyclohexanecarboxyl-CoA hydrolase, at 216,333 to 217,127
GFF4297
GFF4298 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase, at 217,170 to 217,946
GFF4298
Position (kb) 214
215
216
217 Strain fitness (log2 ratio) -1
0
1 at 214.666 kb on + strand, within GFF4295 at 214.733 kb on + strand at 214.734 kb on - strand at 215.137 kb on - strand, within GFF4296 at 215.547 kb on + strand, within GFF4296 at 215.673 kb on + strand, within GFF4296 at 216.017 kb on + strand, within GFF4296 at 216.409 kb on + strand at 216.409 kb on + strand at 216.409 kb on + strand at 216.412 kb on + strand at 216.412 kb on + strand at 216.413 kb on - strand, within GFF4297 at 216.571 kb on - strand, within GFF4297 at 216.613 kb on + strand, within GFF4297 at 216.613 kb on + strand, within GFF4297 at 216.614 kb on - strand, within GFF4297 at 216.890 kb on - strand, within GFF4297 at 217.049 kb on - strand at 217.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 214,666 + GFF4295 0.76 -0.9 214,733 + +0.1 214,734 - +0.4 215,137 - GFF4296 0.24 -0.4 215,547 + GFF4296 0.50 +0.1 215,673 + GFF4296 0.59 -0.9 216,017 + GFF4296 0.81 -0.9 216,409 + -1.0 216,409 + +1.3 216,409 + +0.1 216,412 + +0.2 216,412 + -1.7 216,413 - GFF4297 0.10 -0.1 216,571 - GFF4297 0.30 -0.8 216,613 + GFF4297 0.35 +0.3 216,613 + GFF4297 0.35 +0.3 216,614 - GFF4297 0.35 +0.7 216,890 - GFF4297 0.70 -0.6 217,049 - +0.7 217,115 - -0.6
Or see this region's nucleotide sequence