Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3981 and GFF3982 are separated by 34 nucleotides GFF3982 and GFF3983 overlap by 4 nucleotides
GFF3981 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 184,352 to 185,515
GFF3981
GFF3982 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 185,550 to 186,605
GFF3982
GFF3983 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 186,602 to 188,389
GFF3983
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1
2 at 184.844 kb on + strand, within GFF3981 at 184.844 kb on + strand, within GFF3981 at 184.880 kb on + strand, within GFF3981 at 184.881 kb on - strand, within GFF3981 at 185.070 kb on - strand, within GFF3981 at 185.085 kb on - strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.423 kb on + strand at 185.423 kb on + strand at 185.423 kb on + strand at 185.423 kb on + strand at 185.424 kb on - strand at 185.424 kb on - strand at 185.424 kb on - strand at 185.608 kb on - strand at 186.300 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.367 kb on - strand, within GFF3982 at 187.076 kb on + strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.079 kb on + strand, within GFF3983 at 187.080 kb on - strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.272 kb on - strand, within GFF3983 at 187.331 kb on + strand, within GFF3983
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 184,844 + GFF3981 0.42 -0.1 184,844 + GFF3981 0.42 -1.4 184,880 + GFF3981 0.45 -0.7 184,881 - GFF3981 0.45 +0.7 185,070 - GFF3981 0.62 -0.5 185,085 - GFF3981 0.63 -0.7 185,198 + GFF3981 0.73 -1.8 185,198 + GFF3981 0.73 -1.0 185,198 + GFF3981 0.73 -1.5 185,423 + -0.1 185,423 + +0.2 185,423 + -1.0 185,423 + -1.2 185,424 - -0.1 185,424 - -0.4 185,424 - +0.1 185,608 - -0.1 186,300 + GFF3982 0.71 -0.8 186,363 + GFF3982 0.77 -0.3 186,363 + GFF3982 0.77 -1.7 186,363 + GFF3982 0.77 -0.7 186,363 + GFF3982 0.77 +1.6 186,363 + GFF3982 0.77 -1.1 186,363 + GFF3982 0.77 +2.0 186,364 - GFF3982 0.77 -0.5 186,364 - GFF3982 0.77 -0.6 186,367 - GFF3982 0.77 +0.0 187,076 + GFF3983 0.27 -0.3 187,077 - GFF3983 0.27 +1.1 187,077 - GFF3983 0.27 +0.8 187,077 - GFF3983 0.27 -0.5 187,079 + GFF3983 0.27 -1.3 187,080 - GFF3983 0.27 -1.1 187,271 + GFF3983 0.37 -0.3 187,271 + GFF3983 0.37 +1.2 187,272 - GFF3983 0.37 -1.1 187,331 + GFF3983 0.41 -1.3
Or see this region's nucleotide sequence