Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3972 and GFF3973 overlap by 4 nucleotides GFF3973 and GFF3974 overlap by 4 nucleotides
GFF3972 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 174,520 to 176,148
GFF3972
GFF3973 - FIG01198345: hypothetical protein, at 176,145 to 177,320
GFF3973
GFF3974 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 177,317 to 178,570
GFF3974
Position (kb) 176
177
178 Strain fitness (log2 ratio) -1
0
1
2 at 175.642 kb on + strand, within GFF3972 at 175.990 kb on + strand at 175.991 kb on - strand at 175.993 kb on + strand at 176.008 kb on + strand at 176.208 kb on + strand at 176.209 kb on - strand at 176.565 kb on + strand, within GFF3973 at 176.566 kb on - strand, within GFF3973 at 176.613 kb on + strand, within GFF3973 at 176.613 kb on + strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.614 kb on - strand, within GFF3973 at 176.626 kb on - strand, within GFF3973 at 176.730 kb on + strand, within GFF3973 at 176.731 kb on - strand, within GFF3973 at 176.731 kb on - strand, within GFF3973 at 176.947 kb on - strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.994 kb on + strand, within GFF3973 at 176.995 kb on - strand, within GFF3973 at 177.270 kb on + strand at 177.270 kb on + strand at 177.271 kb on - strand at 177.333 kb on + strand at 177.333 kb on + strand at 177.334 kb on - strand at 178.145 kb on + strand, within GFF3974 at 178.145 kb on + strand, within GFF3974 at 178.146 kb on - strand, within GFF3974 at 178.146 kb on - strand, within GFF3974
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 175,642 + GFF3972 0.69 -1.0 175,990 + +2.2 175,991 - +0.2 175,993 + -0.4 176,008 + -0.8 176,208 + -0.3 176,209 - +0.6 176,565 + GFF3973 0.36 -1.6 176,566 - GFF3973 0.36 -1.1 176,613 + GFF3973 0.40 -0.4 176,613 + GFF3973 0.40 +0.6 176,614 - GFF3973 0.40 +0.2 176,614 - GFF3973 0.40 -0.9 176,614 - GFF3973 0.40 -0.1 176,626 - GFF3973 0.41 -1.1 176,730 + GFF3973 0.50 +1.4 176,731 - GFF3973 0.50 -0.6 176,731 - GFF3973 0.50 -0.7 176,947 - GFF3973 0.68 +1.1 176,994 + GFF3973 0.72 -1.2 176,994 + GFF3973 0.72 -1.3 176,994 + GFF3973 0.72 -0.2 176,995 - GFF3973 0.72 -1.5 177,270 + -0.2 177,270 + -0.3 177,271 - -0.2 177,333 + -0.9 177,333 + -1.3 177,334 - -0.6 178,145 + GFF3974 0.66 +0.2 178,145 + GFF3974 0.66 +0.2 178,146 - GFF3974 0.66 -0.8 178,146 - GFF3974 0.66 -0.7
Or see this region's nucleotide sequence