Strain Fitness in Variovorax sp. SCN45 around GFF3860

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3858 and GFF3859 are separated by 26 nucleotidesGFF3859 and GFF3860 are separated by 99 nucleotidesGFF3860 and GFF3861 are separated by 99 nucleotides GFF3858 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 56,790 to 57,743 GFF3858 GFF3859 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), FadG, at 57,770 to 58,513 GFF3859 GFF3860 - Acyl carrier protein, at 58,613 to 58,852 GFF3860 GFF3861 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 58,952 to 60,196 GFF3861 Position (kb) 58 59Strain fitness (log2 ratio) -1 0 1at 57.759 kb on + strandat 57.759 kb on + strandat 57.759 kb on + strandat 57.759 kb on + strandat 57.759 kb on + strandat 57.759 kb on + strandat 57.759 kb on + strandat 57.769 kb on + strandat 57.769 kb on + strandat 57.769 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.511 kb on + strandat 58.893 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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57,759 + +0.2
57,759 + -0.9
57,759 + -1.0
57,759 + +0.3
57,759 + -0.6
57,759 + +0.0
57,759 + +0.1
57,769 + +0.9
57,769 + -0.7
57,769 + -0.3
58,511 + -0.2
58,511 + +0.3
58,511 + -0.7
58,511 + -0.2
58,511 + +1.6
58,511 + +0.0
58,511 + +0.4
58,511 + +1.0
58,511 + -0.1
58,511 + -0.7
58,511 + -0.3
58,511 + -0.8
58,893 + +1.5

Or see this region's nucleotide sequence