Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3462 and GFF3463 are separated by 4 nucleotides GFF3463 and GFF3464 are separated by 5 nucleotides
GFF3462 - Urea ABC transporter, permease protein UrtC, at 283,791 to 284,846
GFF3462
GFF3463 - Urea ABC transporter, permease protein UrtB, at 284,851 to 285,753
GFF3463
GFF3464 - Urea ABC transporter, substrate-binding protein UrtA, at 285,759 to 286,952
GFF3464
Position (kb)
284
285
286 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 283.897 kb on + strand, within GFF3462 at 283.930 kb on + strand, within GFF3462 at 284.006 kb on - strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.075 kb on - strand, within GFF3462 at 284.146 kb on + strand, within GFF3462 at 284.147 kb on - strand, within GFF3462 at 284.269 kb on + strand, within GFF3462 at 284.419 kb on + strand, within GFF3462 at 284.420 kb on - strand, within GFF3462 at 284.452 kb on + strand, within GFF3462 at 284.464 kb on + strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.894 kb on + strand at 284.894 kb on + strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.954 kb on + strand, within GFF3463 at 284.954 kb on + strand, within GFF3463 at 284.955 kb on - strand, within GFF3463 at 285.059 kb on + strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.254 kb on + strand, within GFF3463 at 285.398 kb on + strand, within GFF3463 at 285.461 kb on + strand, within GFF3463 at 285.495 kb on - strand, within GFF3463 at 285.545 kb on + strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.597 kb on - strand, within GFF3463 at 285.605 kb on + strand, within GFF3463 at 285.606 kb on - strand, within GFF3463 at 285.883 kb on - strand, within GFF3464 at 285.883 kb on - strand, within GFF3464 at 285.989 kb on - strand, within GFF3464 at 286.000 kb on + strand, within GFF3464 at 286.001 kb on - strand, within GFF3464 at 286.001 kb on - strand, within GFF3464 at 286.001 kb on - strand, within GFF3464 at 286.042 kb on + strand, within GFF3464 at 286.042 kb on + strand, within GFF3464 at 286.043 kb on - strand, within GFF3464 at 286.047 kb on - strand, within GFF3464 at 286.047 kb on - strand, within GFF3464 at 286.118 kb on - strand, within GFF3464 at 286.120 kb on + strand, within GFF3464 at 286.120 kb on + strand, within GFF3464 at 286.228 kb on + strand, within GFF3464 at 286.229 kb on - strand, within GFF3464 at 286.229 kb on - strand, within GFF3464 at 286.264 kb on + strand, within GFF3464 at 286.264 kb on + strand, within GFF3464 at 286.264 kb on + strand, within GFF3464 at 286.264 kb on + strand, within GFF3464 at 286.265 kb on - strand, within GFF3464 at 286.265 kb on - strand, within GFF3464 at 286.408 kb on + strand, within GFF3464 at 286.408 kb on + strand, within GFF3464 at 286.414 kb on + strand, within GFF3464 at 286.414 kb on + strand, within GFF3464 at 286.415 kb on - strand, within GFF3464 at 286.540 kb on + strand, within GFF3464 at 286.540 kb on + strand, within GFF3464 at 286.540 kb on + strand, within GFF3464 at 286.541 kb on - strand, within GFF3464 at 286.543 kb on + strand, within GFF3464
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 283,897 + GFF3462 0.10 -1.1 283,930 + GFF3462 0.13 -1.2 284,006 - GFF3462 0.20 +0.4 284,074 + GFF3462 0.27 -1.2 284,074 + GFF3462 0.27 -0.8 284,075 - GFF3462 0.27 -1.0 284,146 + GFF3462 0.34 +2.5 284,147 - GFF3462 0.34 -0.8 284,269 + GFF3462 0.45 -0.6 284,419 + GFF3462 0.59 -0.4 284,420 - GFF3462 0.60 -0.2 284,452 + GFF3462 0.63 +0.0 284,464 + GFF3462 0.64 -1.0 284,465 - GFF3462 0.64 +1.0 284,465 - GFF3462 0.64 +0.2 284,465 - GFF3462 0.64 -0.0 284,465 - GFF3462 0.64 -1.2 284,723 - GFF3462 0.88 -0.0 284,723 - GFF3462 0.88 -1.4 284,723 - GFF3462 0.88 -0.2 284,894 + -0.3 284,894 + +0.4 284,895 - +0.5 284,895 - -1.4 284,895 - +0.7 284,954 + GFF3463 0.11 -0.8 284,954 + GFF3463 0.11 -0.1 284,955 - GFF3463 0.12 +0.6 285,059 + GFF3463 0.23 +0.7 285,060 - GFF3463 0.23 -0.2 285,060 - GFF3463 0.23 -0.2 285,060 - GFF3463 0.23 -0.7 285,060 - GFF3463 0.23 -1.1 285,060 - GFF3463 0.23 +1.8 285,060 - GFF3463 0.23 +0.2 285,254 + GFF3463 0.45 +1.4 285,398 + GFF3463 0.61 +0.8 285,461 + GFF3463 0.68 -1.0 285,495 - GFF3463 0.71 -1.3 285,545 + GFF3463 0.77 +1.0 285,546 - GFF3463 0.77 -0.4 285,546 - GFF3463 0.77 -1.4 285,596 + GFF3463 0.83 +1.2 285,596 + GFF3463 0.83 +0.3 285,597 - GFF3463 0.83 +0.2 285,605 + GFF3463 0.83 +0.6 285,606 - GFF3463 0.84 +1.0 285,883 - GFF3464 0.10 -1.3 285,883 - GFF3464 0.10 +1.3 285,989 - GFF3464 0.19 -1.2 286,000 + GFF3464 0.20 -0.6 286,001 - GFF3464 0.20 -0.7 286,001 - GFF3464 0.20 +0.2 286,001 - GFF3464 0.20 +1.1 286,042 + GFF3464 0.24 -0.0 286,042 + GFF3464 0.24 +0.4 286,043 - GFF3464 0.24 +0.2 286,047 - GFF3464 0.24 -0.2 286,047 - GFF3464 0.24 +1.0 286,118 - GFF3464 0.30 +1.6 286,120 + GFF3464 0.30 +2.0 286,120 + GFF3464 0.30 -0.2 286,228 + GFF3464 0.39 -0.1 286,229 - GFF3464 0.39 -0.1 286,229 - GFF3464 0.39 -0.8 286,264 + GFF3464 0.42 -1.0 286,264 + GFF3464 0.42 -0.8 286,264 + GFF3464 0.42 +0.4 286,264 + GFF3464 0.42 +0.0 286,265 - GFF3464 0.42 -1.1 286,265 - GFF3464 0.42 -0.2 286,408 + GFF3464 0.54 -0.0 286,408 + GFF3464 0.54 -1.8 286,414 + GFF3464 0.55 -0.6 286,414 + GFF3464 0.55 -0.8 286,415 - GFF3464 0.55 +0.2 286,540 + GFF3464 0.65 -0.3 286,540 + GFF3464 0.65 +0.2 286,540 + GFF3464 0.65 +0.4 286,541 - GFF3464 0.65 -0.8 286,543 + GFF3464 0.66 -0.6
Or see this region's nucleotide sequence