Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3048 and GFF3049 are separated by 40 nucleotides GFF3049 and GFF3050 are separated by 166 nucleotides GFF3050 and GFF3051 are separated by 28 nucleotides GFF3051 and GFF3052 are separated by 118 nucleotides
GFF3048 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 483,415 to 484,116
GFF3048
GFF3049 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 484,157 to 484,909
GFF3049
GFF3050 - Lactam utilization protein LamB, at 485,076 to 485,840
GFF3050
GFF3051 - no description, at 485,869 to 486,267
GFF3051
GFF3052 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 486,386 to 487,369
GFF3052
Position (kb) 485
486 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 484.477 kb on - strand, within GFF3049 at 484.893 kb on + strand at 484.894 kb on - strand at 484.924 kb on + strand at 484.925 kb on - strand at 484.957 kb on + strand at 485.015 kb on + strand at 485.016 kb on - strand at 485.037 kb on + strand at 485.037 kb on + strand at 485.038 kb on - strand at 485.038 kb on - strand at 485.051 kb on - strand at 485.575 kb on + strand, within GFF3050 at 485.584 kb on + strand, within GFF3050 at 485.894 kb on - strand at 486.235 kb on + strand at 486.789 kb on + strand, within GFF3052 at 486.789 kb on + strand, within GFF3052 at 486.789 kb on + strand, within GFF3052 at 486.789 kb on + strand, within GFF3052 at 486.790 kb on - strand, within GFF3052 at 486.790 kb on - strand, within GFF3052
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 484,477 - GFF3049 0.42 +0.6 484,893 + -1.7 484,894 - +0.5 484,924 + -0.3 484,925 - -1.8 484,957 + +3.0 485,015 + -0.3 485,016 - -0.1 485,037 + -1.2 485,037 + +0.2 485,038 - -0.4 485,038 - -0.3 485,051 - +0.7 485,575 + GFF3050 0.65 -0.4 485,584 + GFF3050 0.66 -1.4 485,894 - +0.1 486,235 + +0.5 486,789 + GFF3052 0.41 +0.8 486,789 + GFF3052 0.41 -0.2 486,789 + GFF3052 0.41 +0.3 486,789 + GFF3052 0.41 +0.7 486,790 - GFF3052 0.41 -0.7 486,790 - GFF3052 0.41 -0.6
Or see this region's nucleotide sequence