Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3046 and GFF3047 overlap by 4 nucleotides GFF3047 and GFF3048 are separated by 21 nucleotides GFF3048 and GFF3049 are separated by 40 nucleotides GFF3049 and GFF3050 are separated by 166 nucleotides
GFF3046 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 481,826 to 482,617
GFF3046
GFF3047 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 482,614 to 483,393
GFF3047
GFF3048 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 483,415 to 484,116
GFF3048
GFF3049 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 484,157 to 484,909
GFF3049
GFF3050 - Lactam utilization protein LamB, at 485,076 to 485,840
GFF3050
Position (kb) 483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 482.771 kb on + strand, within GFF3047 at 482.772 kb on - strand, within GFF3047 at 482.775 kb on - strand, within GFF3047 at 482.874 kb on - strand, within GFF3047 at 483.071 kb on + strand, within GFF3047 at 483.072 kb on - strand, within GFF3047 at 483.144 kb on - strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.390 kb on - strand at 483.491 kb on + strand, within GFF3048 at 483.492 kb on - strand, within GFF3048 at 483.924 kb on - strand, within GFF3048 at 484.477 kb on - strand, within GFF3049 at 484.893 kb on + strand at 484.894 kb on - strand at 484.924 kb on + strand at 484.925 kb on - strand at 484.957 kb on + strand at 485.015 kb on + strand at 485.016 kb on - strand at 485.037 kb on + strand at 485.037 kb on + strand at 485.038 kb on - strand at 485.038 kb on - strand at 485.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 482,771 + GFF3047 0.20 -0.8 482,772 - GFF3047 0.20 -2.0 482,775 - GFF3047 0.21 +0.4 482,874 - GFF3047 0.33 +0.4 483,071 + GFF3047 0.59 -0.5 483,072 - GFF3047 0.59 +0.3 483,144 - GFF3047 0.68 -1.5 483,188 + GFF3047 0.74 -0.9 483,188 + GFF3047 0.74 -0.4 483,188 + GFF3047 0.74 +0.1 483,188 + GFF3047 0.74 +0.4 483,188 + GFF3047 0.74 +0.4 483,390 - +0.6 483,491 + GFF3048 0.11 +0.5 483,492 - GFF3048 0.11 -0.7 483,924 - GFF3048 0.73 -0.7 484,477 - GFF3049 0.42 +0.6 484,893 + -1.7 484,894 - +0.5 484,924 + -0.3 484,925 - -1.8 484,957 + +3.0 485,015 + -0.3 485,016 - -0.1 485,037 + -1.2 485,037 + +0.2 485,038 - -0.4 485,038 - -0.3 485,051 - +0.7
Or see this region's nucleotide sequence