Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2862 and GFF2863 are separated by 6 nucleotides GFF2863 and GFF2864 are separated by 8 nucleotides GFF2864 and GFF2865 are separated by 14 nucleotides
GFF2862 - FIG01199081: hypothetical protein, at 285,893 to 286,756
GFF2862
GFF2863 - no description, at 286,763 to 287,440
GFF2863
GFF2864 - no description, at 287,449 to 287,967
GFF2864
GFF2865 - Uncharacterized MFS-type transporter, at 287,982 to 289,511
GFF2865
Position (kb)
287
288 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 286.466 kb on + strand, within GFF2862 at 286.466 kb on + strand, within GFF2862 at 286.466 kb on + strand, within GFF2862 at 286.466 kb on + strand, within GFF2862 at 286.467 kb on - strand, within GFF2862 at 286.467 kb on - strand, within GFF2862 at 286.467 kb on - strand, within GFF2862 at 286.623 kb on - strand, within GFF2862 at 286.623 kb on - strand, within GFF2862 at 286.623 kb on - strand, within GFF2862 at 286.623 kb on - strand, within GFF2862 at 286.700 kb on + strand at 286.700 kb on + strand at 286.700 kb on + strand at 286.701 kb on - strand at 286.745 kb on + strand at 286.745 kb on + strand at 286.745 kb on + strand at 286.745 kb on + strand at 286.745 kb on + strand at 286.746 kb on - strand at 286.746 kb on - strand at 287.031 kb on + strand, within GFF2863 at 287.031 kb on + strand, within GFF2863 at 287.031 kb on + strand, within GFF2863 at 287.031 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.073 kb on + strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.074 kb on - strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.223 kb on + strand, within GFF2863 at 287.224 kb on - strand, within GFF2863 at 287.224 kb on - strand, within GFF2863 at 287.224 kb on - strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.229 kb on + strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.230 kb on - strand, within GFF2863 at 287.603 kb on - strand, within GFF2864 at 287.603 kb on - strand, within GFF2864 at 287.603 kb on - strand, within GFF2864 at 287.603 kb on - strand, within GFF2864 at 287.603 kb on - strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.664 kb on + strand, within GFF2864 at 287.665 kb on - strand, within GFF2864 at 287.665 kb on - strand, within GFF2864 at 287.665 kb on - strand, within GFF2864 at 287.665 kb on - strand, within GFF2864 at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.954 kb on + strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 287.955 kb on - strand at 288.430 kb on + strand, within GFF2865 at 288.430 kb on + strand, within GFF2865 at 288.430 kb on + strand, within GFF2865 at 288.431 kb on - strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.485 kb on + strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.486 kb on - strand, within GFF2865 at 288.487 kb on + strand, within GFF2865 at 288.487 kb on + strand, within GFF2865 at 288.655 kb on + strand, within GFF2865 at 288.655 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.853 kb on + strand, within GFF2865 at 288.854 kb on - strand, within GFF2865 at 288.854 kb on - strand, within GFF2865 at 288.854 kb on - strand, within GFF2865 at 288.854 kb on - strand, within GFF2865 at 288.881 kb on + strand, within GFF2865 at 288.881 kb on + strand, within GFF2865 at 288.881 kb on + strand, within GFF2865 at 288.882 kb on - strand, within GFF2865 at 288.882 kb on - strand, within GFF2865 at 288.882 kb on - strand, within GFF2865
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 286,466 + GFF2862 0.66 +0.2 286,466 + GFF2862 0.66 -1.0 286,466 + GFF2862 0.66 -0.4 286,466 + GFF2862 0.66 -0.2 286,467 - GFF2862 0.66 +0.0 286,467 - GFF2862 0.66 -0.2 286,467 - GFF2862 0.66 -1.7 286,623 - GFF2862 0.84 -0.4 286,623 - GFF2862 0.84 -1.1 286,623 - GFF2862 0.84 +1.6 286,623 - GFF2862 0.84 +0.3 286,700 + -1.0 286,700 + +0.8 286,700 + -0.7 286,701 - -0.5 286,745 + -1.1 286,745 + -1.8 286,745 + -0.7 286,745 + +0.1 286,745 + +0.4 286,746 - -0.2 286,746 - -0.4 287,031 + GFF2863 0.40 +1.0 287,031 + GFF2863 0.40 -1.4 287,031 + GFF2863 0.40 -0.7 287,031 + GFF2863 0.40 -0.5 287,073 + GFF2863 0.46 +1.0 287,073 + GFF2863 0.46 -0.7 287,073 + GFF2863 0.46 +0.6 287,073 + GFF2863 0.46 -0.7 287,073 + GFF2863 0.46 -0.3 287,073 + GFF2863 0.46 +1.6 287,073 + GFF2863 0.46 +0.6 287,073 + GFF2863 0.46 -1.0 287,074 - GFF2863 0.46 -0.5 287,074 - GFF2863 0.46 +0.0 287,074 - GFF2863 0.46 -0.4 287,074 - GFF2863 0.46 +0.7 287,074 - GFF2863 0.46 +0.4 287,074 - GFF2863 0.46 -0.1 287,074 - GFF2863 0.46 -0.7 287,074 - GFF2863 0.46 +0.0 287,074 - GFF2863 0.46 +0.2 287,223 + GFF2863 0.68 -1.4 287,223 + GFF2863 0.68 -0.1 287,223 + GFF2863 0.68 +1.6 287,223 + GFF2863 0.68 -0.5 287,223 + GFF2863 0.68 -0.6 287,223 + GFF2863 0.68 -0.3 287,224 - GFF2863 0.68 -1.0 287,224 - GFF2863 0.68 -0.5 287,224 - GFF2863 0.68 +0.3 287,229 + GFF2863 0.69 +0.4 287,229 + GFF2863 0.69 +0.2 287,229 + GFF2863 0.69 +0.4 287,229 + GFF2863 0.69 +0.5 287,229 + GFF2863 0.69 -0.1 287,229 + GFF2863 0.69 -0.2 287,229 + GFF2863 0.69 +0.4 287,229 + GFF2863 0.69 -0.6 287,229 + GFF2863 0.69 -1.0 287,229 + GFF2863 0.69 -0.2 287,229 + GFF2863 0.69 -0.0 287,229 + GFF2863 0.69 +0.8 287,229 + GFF2863 0.69 -1.2 287,229 + GFF2863 0.69 +0.8 287,229 + GFF2863 0.69 -1.2 287,229 + GFF2863 0.69 -0.6 287,229 + GFF2863 0.69 +0.9 287,230 - GFF2863 0.69 +0.6 287,230 - GFF2863 0.69 -2.4 287,230 - GFF2863 0.69 -0.2 287,230 - GFF2863 0.69 -0.4 287,230 - GFF2863 0.69 +0.3 287,230 - GFF2863 0.69 -0.1 287,230 - GFF2863 0.69 -0.7 287,230 - GFF2863 0.69 +1.8 287,230 - GFF2863 0.69 -0.3 287,230 - GFF2863 0.69 -0.3 287,230 - GFF2863 0.69 -0.3 287,230 - GFF2863 0.69 +0.9 287,230 - GFF2863 0.69 +0.0 287,230 - GFF2863 0.69 -0.0 287,230 - GFF2863 0.69 +0.2 287,230 - GFF2863 0.69 +1.9 287,230 - GFF2863 0.69 -1.4 287,230 - GFF2863 0.69 -2.8 287,230 - GFF2863 0.69 +0.2 287,230 - GFF2863 0.69 +0.8 287,230 - GFF2863 0.69 -0.6 287,230 - GFF2863 0.69 -0.8 287,230 - GFF2863 0.69 +0.6 287,603 - GFF2864 0.30 +1.0 287,603 - GFF2864 0.30 +0.6 287,603 - GFF2864 0.30 +0.6 287,603 - GFF2864 0.30 -0.5 287,603 - GFF2864 0.30 +1.0 287,664 + GFF2864 0.41 +0.6 287,664 + GFF2864 0.41 -0.6 287,664 + GFF2864 0.41 +1.0 287,664 + GFF2864 0.41 -1.8 287,664 + GFF2864 0.41 +0.3 287,664 + GFF2864 0.41 +0.3 287,664 + GFF2864 0.41 -0.1 287,664 + GFF2864 0.41 -0.1 287,664 + GFF2864 0.41 -1.7 287,664 + GFF2864 0.41 -1.5 287,664 + GFF2864 0.41 -0.7 287,664 + GFF2864 0.41 -1.1 287,665 - GFF2864 0.42 +1.6 287,665 - GFF2864 0.42 -0.6 287,665 - GFF2864 0.42 +0.8 287,665 - GFF2864 0.42 +2.0 287,954 + -0.9 287,954 + -0.3 287,954 + -1.4 287,954 + +0.3 287,954 + +0.4 287,954 + -0.6 287,954 + -0.5 287,954 + -0.4 287,955 - +0.3 287,955 - +0.5 287,955 - -1.7 287,955 - +0.2 287,955 - +0.3 287,955 - +0.0 287,955 - -0.8 287,955 - +0.0 287,955 - +0.6 287,955 - -1.7 287,955 - +0.6 287,955 - -0.4 288,430 + GFF2865 0.29 -0.5 288,430 + GFF2865 0.29 -1.4 288,430 + GFF2865 0.29 +0.6 288,431 - GFF2865 0.29 -0.1 288,485 + GFF2865 0.33 -0.8 288,485 + GFF2865 0.33 +0.2 288,485 + GFF2865 0.33 -0.4 288,485 + GFF2865 0.33 -2.3 288,485 + GFF2865 0.33 +0.3 288,485 + GFF2865 0.33 -1.8 288,485 + GFF2865 0.33 -0.4 288,486 - GFF2865 0.33 +1.0 288,486 - GFF2865 0.33 -0.7 288,486 - GFF2865 0.33 -0.4 288,486 - GFF2865 0.33 +0.6 288,486 - GFF2865 0.33 -0.2 288,486 - GFF2865 0.33 -0.3 288,486 - GFF2865 0.33 +1.1 288,486 - GFF2865 0.33 -0.1 288,486 - GFF2865 0.33 -2.7 288,486 - GFF2865 0.33 -0.8 288,486 - GFF2865 0.33 -0.3 288,486 - GFF2865 0.33 -1.1 288,487 + GFF2865 0.33 -1.2 288,487 + GFF2865 0.33 -0.4 288,655 + GFF2865 0.44 +1.3 288,655 + GFF2865 0.44 +0.4 288,853 + GFF2865 0.57 -0.2 288,853 + GFF2865 0.57 +0.7 288,853 + GFF2865 0.57 -0.3 288,853 + GFF2865 0.57 -0.7 288,853 + GFF2865 0.57 +0.0 288,853 + GFF2865 0.57 +0.0 288,853 + GFF2865 0.57 -0.9 288,853 + GFF2865 0.57 -0.1 288,854 - GFF2865 0.57 -0.3 288,854 - GFF2865 0.57 -0.1 288,854 - GFF2865 0.57 -1.6 288,854 - GFF2865 0.57 +0.1 288,881 + GFF2865 0.59 +0.2 288,881 + GFF2865 0.59 -0.5 288,881 + GFF2865 0.59 -1.1 288,882 - GFF2865 0.59 -0.1 288,882 - GFF2865 0.59 +0.5 288,882 - GFF2865 0.59 -0.5
Or see this region's nucleotide sequence