Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2701 and GFF2702 overlap by 4 nucleotides GFF2702 and GFF2703 are separated by 65 nucleotides GFF2703 and GFF2704 are separated by 10 nucleotides
GFF2701 - CaiB/BaiF family protein, at 128,391 to 129,560
GFF2701
GFF2702 - FAD-dependent oxidoreductase, at 129,557 to 130,939
GFF2702
GFF2703 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 131,005 to 131,868
GFF2703
GFF2704 - Hydroxymethylpyrimidine ABC transporter, ATP-binding protein, at 131,879 to 132,673
GFF2704
Position (kb) 129
130
131 Strain fitness (log2 ratio) -1
0
1
2 at 128.741 kb on - strand, within GFF2701 at 129.184 kb on + strand, within GFF2701 at 129.184 kb on + strand, within GFF2701 at 129.184 kb on + strand, within GFF2701 at 129.856 kb on - strand, within GFF2702 at 129.858 kb on + strand, within GFF2702 at 129.859 kb on - strand, within GFF2702 at 129.859 kb on - strand, within GFF2702 at 129.862 kb on - strand, within GFF2702 at 129.862 kb on - strand, within GFF2702 at 129.930 kb on + strand, within GFF2702 at 130.296 kb on + strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.578 kb on + strand, within GFF2702 at 130.578 kb on + strand, within GFF2702 at 130.579 kb on - strand, within GFF2702 at 130.579 kb on - strand, within GFF2702 at 130.785 kb on + strand, within GFF2702 at 130.786 kb on - strand, within GFF2702 at 130.930 kb on + strand at 130.940 kb on - strand at 131.627 kb on + strand, within GFF2703
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 128,741 - GFF2701 0.30 +0.4 129,184 + GFF2701 0.68 -0.3 129,184 + GFF2701 0.68 +0.2 129,184 + GFF2701 0.68 -1.5 129,856 - GFF2702 0.22 +0.5 129,858 + GFF2702 0.22 -1.0 129,859 - GFF2702 0.22 -1.2 129,859 - GFF2702 0.22 +0.7 129,862 - GFF2702 0.22 -0.7 129,862 - GFF2702 0.22 +0.6 129,930 + GFF2702 0.27 +0.7 130,296 + GFF2702 0.53 +0.2 130,297 - GFF2702 0.54 +0.6 130,297 - GFF2702 0.54 -1.6 130,297 - GFF2702 0.54 +1.0 130,578 + GFF2702 0.74 +2.0 130,578 + GFF2702 0.74 +0.2 130,579 - GFF2702 0.74 +0.3 130,579 - GFF2702 0.74 -0.1 130,785 + GFF2702 0.89 -1.2 130,786 - GFF2702 0.89 -0.0 130,930 + -0.4 130,940 - +0.2 131,627 + GFF2703 0.72 -1.7
Or see this region's nucleotide sequence