Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2421 and GFF2422 overlap by 4 nucleotides GFF2422 and GFF2423 are separated by 36 nucleotides GFF2423 and GFF2424 overlap by 4 nucleotides GFF2424 and GFF2425 overlap by 4 nucleotides
GFF2421 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 472,636 to 473,397
GFF2421
GFF2422 - Polysaccharide deacetylase, at 473,394 to 474,218
GFF2422
GFF2423 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 474,255 to 475,046
GFF2423
GFF2424 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 475,043 to 475,894
GFF2424
GFF2425 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 475,891 to 476,982
GFF2425
Position (kb) 474
475
476 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 473.379 kb on + strand at 473.379 kb on + strand at 473.657 kb on - strand, within GFF2422 at 473.741 kb on - strand, within GFF2422 at 473.773 kb on + strand, within GFF2422 at 473.773 kb on + strand, within GFF2422 at 473.785 kb on + strand, within GFF2422 at 473.785 kb on + strand, within GFF2422 at 473.786 kb on - strand, within GFF2422 at 473.944 kb on + strand, within GFF2422 at 473.945 kb on - strand, within GFF2422 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.604 kb on - strand, within GFF2423 at 474.607 kb on + strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.676 kb on + strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.784 kb on + strand, within GFF2423 at 474.785 kb on - strand, within GFF2423 at 474.931 kb on + strand, within GFF2423 at 474.932 kb on - strand, within GFF2423 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.194 kb on + strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.339 kb on - strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.710 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.714 kb on - strand, within GFF2424 at 475.737 kb on + strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.783 kb on - strand, within GFF2424 at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 476.042 kb on + strand, within GFF2425 at 476.042 kb on + strand, within GFF2425 at 476.043 kb on - strand, within GFF2425
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 473,379 + +0.4 473,379 + -0.4 473,657 - GFF2422 0.32 -0.8 473,741 - GFF2422 0.42 -1.7 473,773 + GFF2422 0.46 -1.2 473,773 + GFF2422 0.46 -0.8 473,785 + GFF2422 0.47 -1.5 473,785 + GFF2422 0.47 +1.8 473,786 - GFF2422 0.48 +0.8 473,944 + GFF2422 0.67 -1.9 473,945 - GFF2422 0.67 -0.7 474,603 + GFF2423 0.44 -0.2 474,603 + GFF2423 0.44 +0.3 474,603 + GFF2423 0.44 -0.9 474,603 + GFF2423 0.44 +0.8 474,603 + GFF2423 0.44 +0.1 474,604 - GFF2423 0.44 -0.6 474,607 + GFF2423 0.44 -0.2 474,608 - GFF2423 0.45 +1.8 474,608 - GFF2423 0.45 -1.3 474,676 + GFF2423 0.53 +1.5 474,680 - GFF2423 0.54 +0.2 474,680 - GFF2423 0.54 +1.2 474,680 - GFF2423 0.54 -0.2 474,784 + GFF2423 0.67 -0.2 474,785 - GFF2423 0.67 -0.4 474,931 + GFF2423 0.85 -0.5 474,932 - GFF2423 0.85 -0.8 475,170 + GFF2424 0.15 +0.4 475,170 + GFF2424 0.15 -1.0 475,170 + GFF2424 0.15 +0.8 475,194 + GFF2424 0.18 +0.3 475,195 - GFF2424 0.18 -0.0 475,195 - GFF2424 0.18 +0.0 475,339 - GFF2424 0.35 -0.3 475,626 + GFF2424 0.68 -1.7 475,626 + GFF2424 0.68 -0.3 475,626 + GFF2424 0.68 -0.3 475,626 + GFF2424 0.68 +0.1 475,627 - GFF2424 0.69 +0.5 475,627 - GFF2424 0.69 +0.2 475,627 - GFF2424 0.69 -0.6 475,627 - GFF2424 0.69 -0.2 475,627 - GFF2424 0.69 -0.3 475,630 - GFF2424 0.69 -1.0 475,630 - GFF2424 0.69 -0.1 475,710 + GFF2424 0.78 -1.5 475,713 + GFF2424 0.79 -2.1 475,713 + GFF2424 0.79 +0.4 475,713 + GFF2424 0.79 +0.0 475,714 - GFF2424 0.79 -0.5 475,737 + GFF2424 0.81 -0.3 475,738 - GFF2424 0.82 +1.0 475,738 - GFF2424 0.82 -0.5 475,738 - GFF2424 0.82 -0.3 475,783 - GFF2424 0.87 -1.0 475,928 + +0.1 475,928 + +0.5 475,928 + -1.4 475,928 + +0.5 475,929 - +0.2 475,929 - +0.1 475,929 - -2.8 475,929 - +2.5 475,929 - +0.0 475,929 - -0.7 475,929 - -1.0 476,042 + GFF2425 0.14 +1.1 476,042 + GFF2425 0.14 -0.5 476,043 - GFF2425 0.14 -0.1
Or see this region's nucleotide sequence