Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2355 and GFF2356 are separated by 6 nucleotides GFF2356 and GFF2357 overlap by 4 nucleotides GFF2357 and GFF2358 are separated by 1 nucleotides GFF2358 and GFF2359 are separated by 31 nucleotides
GFF2355 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 406,416 to 407,462
GFF2355
GFF2356 - RNase adapter protein RapZ, at 407,469 to 408,347
GFF2356
GFF2357 - DNA repair protein RecN, at 408,344 to 410,026
GFF2357
GFF2358 - NAD kinase (EC 2.7.1.23), at 410,028 to 410,939
GFF2358
GFF2359 - Heat-inducible transcription repressor HrcA, at 410,971 to 411,978
GFF2359
Position (kb) 408
409
410
411 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.460 kb on + strand at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.596 kb on + strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.597 kb on - strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.659 kb on + strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.660 kb on - strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.743 kb on + strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.744 kb on - strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.779 kb on + strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.780 kb on - strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.872 kb on + strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 407.873 kb on - strand, within GFF2356 at 408.049 kb on + strand, within GFF2356 at 408.049 kb on + strand, within GFF2356 at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.262 kb on + strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.263 kb on - strand at 408.265 kb on + strand at 408.266 kb on - strand at 408.266 kb on - strand at 408.809 kb on + strand, within GFF2357 at 408.810 kb on - strand, within GFF2357 at 408.810 kb on - strand, within GFF2357 at 408.972 kb on + strand, within GFF2357 at 408.972 kb on + strand, within GFF2357 at 408.972 kb on + strand, within GFF2357 at 408.972 kb on + strand, within GFF2357 at 408.972 kb on + strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 408.973 kb on - strand, within GFF2357 at 409.095 kb on + strand, within GFF2357 at 409.095 kb on + strand, within GFF2357 at 409.096 kb on - strand, within GFF2357 at 409.584 kb on + strand, within GFF2357 at 409.584 kb on + strand, within GFF2357 at 410.939 kb on + strand at 410.939 kb on + strand at 410.939 kb on + strand at 410.939 kb on + strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.940 kb on - strand at 410.951 kb on + strand at 410.952 kb on - strand at 410.952 kb on - strand at 410.952 kb on - strand at 410.959 kb on + strand at 410.959 kb on + strand at 410.959 kb on + strand at 410.960 kb on - strand at 410.960 kb on - strand at 411.019 kb on + strand at 411.019 kb on + strand at 411.019 kb on + strand at 411.019 kb on + strand at 411.020 kb on - strand at 411.020 kb on - strand at 411.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 407,460 + +0.2 407,596 + GFF2356 0.14 -3.1 407,596 + GFF2356 0.14 -0.2 407,596 + GFF2356 0.14 -0.3 407,596 + GFF2356 0.14 -0.3 407,596 + GFF2356 0.14 -0.9 407,596 + GFF2356 0.14 -0.1 407,597 - GFF2356 0.15 -1.2 407,597 - GFF2356 0.15 -2.0 407,597 - GFF2356 0.15 -0.2 407,597 - GFF2356 0.15 -1.2 407,597 - GFF2356 0.15 -1.0 407,659 + GFF2356 0.22 +0.5 407,659 + GFF2356 0.22 -1.0 407,659 + GFF2356 0.22 -1.5 407,659 + GFF2356 0.22 -0.8 407,659 + GFF2356 0.22 -1.0 407,659 + GFF2356 0.22 -1.0 407,660 - GFF2356 0.22 -0.4 407,660 - GFF2356 0.22 -0.2 407,660 - GFF2356 0.22 -0.2 407,660 - GFF2356 0.22 -0.7 407,660 - GFF2356 0.22 -1.3 407,660 - GFF2356 0.22 -0.5 407,743 + GFF2356 0.31 +0.2 407,743 + GFF2356 0.31 -1.1 407,743 + GFF2356 0.31 +0.8 407,743 + GFF2356 0.31 -1.4 407,743 + GFF2356 0.31 +0.6 407,743 + GFF2356 0.31 +0.2 407,743 + GFF2356 0.31 -0.3 407,744 - GFF2356 0.31 -1.2 407,744 - GFF2356 0.31 -0.5 407,744 - GFF2356 0.31 +0.2 407,779 + GFF2356 0.35 -2.5 407,779 + GFF2356 0.35 +0.8 407,779 + GFF2356 0.35 -2.2 407,780 - GFF2356 0.35 -0.2 407,780 - GFF2356 0.35 +0.2 407,780 - GFF2356 0.35 -0.7 407,780 - GFF2356 0.35 -1.0 407,780 - GFF2356 0.35 -1.0 407,872 + GFF2356 0.46 -1.8 407,872 + GFF2356 0.46 +0.5 407,872 + GFF2356 0.46 -1.9 407,872 + GFF2356 0.46 -1.2 407,873 - GFF2356 0.46 -1.0 407,873 - GFF2356 0.46 -0.0 407,873 - GFF2356 0.46 -2.8 407,873 - GFF2356 0.46 -0.6 407,873 - GFF2356 0.46 -1.5 407,873 - GFF2356 0.46 -0.8 407,873 - GFF2356 0.46 -2.1 407,873 - GFF2356 0.46 -1.5 407,873 - GFF2356 0.46 -0.8 407,873 - GFF2356 0.46 -0.1 407,873 - GFF2356 0.46 -0.4 407,873 - GFF2356 0.46 +0.2 408,049 + GFF2356 0.66 +0.2 408,049 + GFF2356 0.66 -1.1 408,262 + -0.6 408,262 + -1.1 408,262 + -0.6 408,262 + -1.2 408,262 + -1.5 408,263 - -0.2 408,263 - -1.2 408,263 - -1.5 408,263 - -0.8 408,263 - -0.7 408,263 - -1.7 408,265 + -2.1 408,266 - -0.1 408,266 - +0.1 408,809 + GFF2357 0.28 +1.1 408,810 - GFF2357 0.28 +1.0 408,810 - GFF2357 0.28 -0.2 408,972 + GFF2357 0.37 +0.5 408,972 + GFF2357 0.37 +3.1 408,972 + GFF2357 0.37 +0.2 408,972 + GFF2357 0.37 -0.4 408,972 + GFF2357 0.37 -0.5 408,973 - GFF2357 0.37 -0.2 408,973 - GFF2357 0.37 -0.2 408,973 - GFF2357 0.37 -0.1 408,973 - GFF2357 0.37 +0.8 408,973 - GFF2357 0.37 +0.3 408,973 - GFF2357 0.37 -0.1 409,095 + GFF2357 0.45 -0.9 409,095 + GFF2357 0.45 +1.6 409,096 - GFF2357 0.45 +0.1 409,584 + GFF2357 0.74 +0.6 409,584 + GFF2357 0.74 +0.4 410,939 + +0.6 410,939 + -0.4 410,939 + +1.1 410,939 + -1.0 410,940 - -0.3 410,940 - -0.1 410,940 - +0.2 410,940 - +0.2 410,940 - +0.2 410,940 - -1.8 410,940 - +0.9 410,940 - -0.2 410,951 + -0.0 410,952 - -0.0 410,952 - +0.4 410,952 - -0.7 410,959 + -0.2 410,959 + +0.1 410,959 + +1.8 410,960 - -0.5 410,960 - +0.1 411,019 + -0.5 411,019 + +0.4 411,019 + +0.5 411,019 + -1.7 411,020 - -2.4 411,020 - -0.6 411,020 - +0.2
Or see this region's nucleotide sequence