Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2302 and GFF2303 are separated by 77 nucleotides GFF2303 and GFF2304 are separated by 36 nucleotides GFF2304 and GFF2305 overlap by 14 nucleotides
GFF2302 - Cytochrome c oxidase polypeptide III (EC 1.9.3.1), at 354,470 to 355,351
GFF2302
GFF2303 - Uncharacterized DUF2909 protein BB4826, at 355,429 to 355,650
GFF2303
GFF2304 - Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion, at 355,687 to 356,541
GFF2304
GFF2305 - Hypothetical protein in cytochrome oxidase biogenesis cluster, at 356,528 to 357,208
GFF2305
Position (kb) 355
356 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 354.469 kb on + strand at 354.469 kb on + strand at 354.469 kb on + strand at 354.469 kb on + strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.500 kb on + strand at 354.500 kb on + strand at 354.500 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.696 kb on - strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.743 kb on - strand, within GFF2302 at 354.743 kb on - strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.095 kb on - strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.292 kb on + strand at 355.292 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.340 kb on + strand at 355.341 kb on - strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.643 kb on + strand at 355.644 kb on - strand at 355.644 kb on - strand at 355.644 kb on - strand at 355.660 kb on + strand at 355.660 kb on + strand at 355.661 kb on - strand at 355.661 kb on - strand at 355.661 kb on - strand at 355.705 kb on + strand at 355.705 kb on + strand at 355.705 kb on + strand at 355.706 kb on - strand at 355.706 kb on - strand at 355.907 kb on - strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.964 kb on - strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.269 kb on + strand, within GFF2304 at 356.270 kb on - strand, within GFF2304 at 356.335 kb on - strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.429 kb on - strand, within GFF2304 at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 354,469 + -0.6 354,469 + -1.7 354,469 + -0.2 354,469 + -0.1 354,470 - -0.6 354,470 - -0.5 354,470 - +0.4 354,470 - +0.5 354,470 - -2.1 354,470 - -0.8 354,470 - -1.1 354,500 + -1.1 354,500 + -1.0 354,500 + -0.7 354,503 + -0.5 354,503 + -3.3 354,503 + -0.5 354,503 + -1.7 354,503 + -1.0 354,503 + -0.5 354,504 - -0.0 354,504 - -2.3 354,504 - -1.5 354,504 - -1.8 354,504 - -2.2 354,504 - -0.9 354,504 - +0.3 354,696 - GFF2302 0.26 -1.9 354,742 + GFF2302 0.31 -2.3 354,742 + GFF2302 0.31 -1.4 354,742 + GFF2302 0.31 -0.9 354,742 + GFF2302 0.31 -0.6 354,742 + GFF2302 0.31 -0.5 354,743 - GFF2302 0.31 -1.8 354,743 - GFF2302 0.31 +0.8 355,094 + GFF2302 0.71 -2.0 355,094 + GFF2302 0.71 -1.6 355,094 + GFF2302 0.71 -0.7 355,095 - GFF2302 0.71 -1.0 355,100 + GFF2302 0.71 -0.5 355,100 + GFF2302 0.71 -2.2 355,100 + GFF2302 0.71 -0.5 355,100 + GFF2302 0.71 -0.3 355,100 + GFF2302 0.71 -1.1 355,101 - GFF2302 0.72 -1.2 355,101 - GFF2302 0.72 -0.2 355,101 - GFF2302 0.72 +1.2 355,101 - GFF2302 0.72 -1.4 355,112 + GFF2302 0.73 -1.4 355,112 + GFF2302 0.73 -1.0 355,112 + GFF2302 0.73 -0.5 355,113 - GFF2302 0.73 -0.8 355,113 - GFF2302 0.73 -0.6 355,113 - GFF2302 0.73 +0.9 355,113 - GFF2302 0.73 -0.8 355,113 - GFF2302 0.73 -0.5 355,148 + GFF2302 0.77 -0.4 355,148 + GFF2302 0.77 +0.2 355,148 + GFF2302 0.77 -0.8 355,148 + GFF2302 0.77 -1.3 355,149 - GFF2302 0.77 -1.8 355,149 - GFF2302 0.77 -2.5 355,149 - GFF2302 0.77 -1.0 355,149 - GFF2302 0.77 +0.6 355,149 - GFF2302 0.77 -1.5 355,292 + -0.9 355,292 + -1.8 355,328 + -1.0 355,328 + -0.9 355,328 + -0.4 355,328 + -1.3 355,328 + -1.8 355,328 + -0.4 355,328 + -0.6 355,328 + +1.3 355,329 - -1.4 355,329 - -0.8 355,329 - -0.8 355,329 - -1.3 355,329 - -1.0 355,329 - -1.1 355,329 - -0.3 355,340 + +0.0 355,341 - -0.9 355,430 + -0.5 355,430 + -0.5 355,430 + -0.7 355,430 + +0.2 355,430 + -0.2 355,430 + -0.7 355,430 + -0.2 355,430 + +0.0 355,430 + +0.3 355,430 + -0.3 355,430 + +0.1 355,430 + -0.7 355,430 + -0.5 355,431 - -0.1 355,431 - -0.2 355,431 - -1.0 355,431 - -1.3 355,431 - -0.9 355,431 - -0.3 355,431 - -0.7 355,431 - -1.4 355,431 - -0.0 355,431 - +0.8 355,431 - -0.6 355,463 + GFF2303 0.15 -0.1 355,463 + GFF2303 0.15 -1.5 355,463 + GFF2303 0.15 -0.4 355,463 + GFF2303 0.15 +0.0 355,463 + GFF2303 0.15 +0.1 355,463 + GFF2303 0.15 -0.7 355,463 + GFF2303 0.15 +0.5 355,464 - GFF2303 0.16 -1.3 355,464 - GFF2303 0.16 -0.0 355,464 - GFF2303 0.16 -1.3 355,464 - GFF2303 0.16 +0.6 355,464 - GFF2303 0.16 -0.2 355,643 + -1.0 355,644 - -0.0 355,644 - -1.9 355,644 - -1.8 355,660 + -0.7 355,660 + -1.0 355,661 - -1.5 355,661 - -0.3 355,661 - +1.1 355,705 + +0.3 355,705 + -0.5 355,705 + -0.0 355,706 - -1.5 355,706 - -1.3 355,907 - GFF2304 0.26 -0.6 355,963 + GFF2304 0.32 -0.6 355,963 + GFF2304 0.32 -1.0 355,963 + GFF2304 0.32 -1.7 355,964 - GFF2304 0.32 -0.5 356,002 + GFF2304 0.37 -0.9 356,002 + GFF2304 0.37 -0.5 356,002 + GFF2304 0.37 -1.2 356,002 + GFF2304 0.37 -0.7 356,002 + GFF2304 0.37 -1.5 356,002 + GFF2304 0.37 -0.6 356,003 - GFF2304 0.37 -1.7 356,003 - GFF2304 0.37 -1.0 356,003 - GFF2304 0.37 -1.8 356,003 - GFF2304 0.37 -2.1 356,003 - GFF2304 0.37 -1.8 356,003 - GFF2304 0.37 -0.7 356,269 + GFF2304 0.68 +0.9 356,270 - GFF2304 0.68 -0.6 356,335 - GFF2304 0.76 -0.6 356,422 + GFF2304 0.86 -1.4 356,422 + GFF2304 0.86 -0.4 356,422 + GFF2304 0.86 -0.2 356,422 + GFF2304 0.86 -0.6 356,422 + GFF2304 0.86 +0.2 356,422 + GFF2304 0.86 -0.4 356,422 + GFF2304 0.86 -2.4 356,422 + GFF2304 0.86 -0.9 356,422 + GFF2304 0.86 -0.4 356,423 - GFF2304 0.86 -0.6 356,423 - GFF2304 0.86 -0.0 356,423 - GFF2304 0.86 -0.9 356,423 - GFF2304 0.86 -2.3 356,429 - GFF2304 0.87 -0.4 356,473 + -1.0 356,473 + -3.5 356,473 + -0.9 356,473 + +0.7 356,473 + -1.0 356,473 + -0.3 356,473 + -1.1 356,473 + -0.2 356,474 - -1.4 356,474 - -1.4 356,474 - -0.0 356,474 - +0.2 356,474 - -1.6 356,474 - -0.9 356,474 - -0.8 356,474 - -0.7 356,474 - -0.7 356,474 - -0.7 356,474 - -1.9 356,474 - -0.9 356,474 - -0.6 356,474 - +1.2
Or see this region's nucleotide sequence