Strain Fitness in Variovorax sp. SCN45 around GFF2266

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2265 and GFF2266 are separated by 5 nucleotidesGFF2266 and GFF2267 are separated by 90 nucleotidesGFF2267 and GFF2268 are separated by 100 nucleotides GFF2265 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 320,523 to 321,356 GFF2265 GFF2266 - FIG00441126: hypothetical protein, at 321,362 to 321,967 GFF2266 GFF2267 - Integral membrane protein, at 322,058 to 322,816 GFF2267 GFF2268 - Protein of unknown function DUF1428, at 322,917 to 323,276 GFF2268 Position (kb) 321 322Strain fitness (log2 ratio) -2 -1 0 1 2at 320.483 kb on + strandat 320.484 kb on - strandat 320.688 kb on + strand, within GFF2265at 320.688 kb on + strand, within GFF2265at 320.688 kb on + strand, within GFF2265at 320.689 kb on - strand, within GFF2265at 320.689 kb on - strand, within GFF2265at 320.835 kb on + strand, within GFF2265at 320.835 kb on + strand, within GFF2265at 320.910 kb on + strand, within GFF2265at 320.910 kb on + strand, within GFF2265at 320.910 kb on + strand, within GFF2265at 320.910 kb on + strand, within GFF2265at 320.911 kb on - strand, within GFF2265at 320.911 kb on - strand, within GFF2265at 320.911 kb on - strand, within GFF2265at 320.991 kb on + strand, within GFF2265at 320.991 kb on + strand, within GFF2265at 320.992 kb on - strand, within GFF2265at 321.093 kb on + strand, within GFF2265at 321.195 kb on + strand, within GFF2265at 321.506 kb on + strand, within GFF2266at 321.506 kb on + strand, within GFF2266at 321.689 kb on + strand, within GFF2266at 321.690 kb on - strand, within GFF2266at 321.746 kb on + strand, within GFF2266at 321.746 kb on + strand, within GFF2266at 321.746 kb on + strand, within GFF2266at 321.747 kb on - strand, within GFF2266at 321.747 kb on - strand, within GFF2266at 321.845 kb on + strand, within GFF2266at 321.845 kb on + strand, within GFF2266at 321.845 kb on + strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.846 kb on - strand, within GFF2266at 321.920 kb on + strandat 321.966 kb on - strandat 322.008 kb on + strandat 322.085 kb on + strandat 322.085 kb on + strandat 322.086 kb on - strandat 322.086 kb on - strandat 322.086 kb on - strandat 322.091 kb on + strandat 322.091 kb on + strandat 322.092 kb on - strandat 322.092 kb on - strandat 322.535 kb on + strand, within GFF2267at 322.535 kb on + strand, within GFF2267at 322.535 kb on + strand, within GFF2267at 322.535 kb on + strand, within GFF2267at 322.535 kb on + strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.536 kb on - strand, within GFF2267at 322.586 kb on + strand, within GFF2267at 322.586 kb on + strand, within GFF2267at 322.586 kb on + strand, within GFF2267at 322.587 kb on - strand, within GFF2267at 322.587 kb on - strand, within GFF2267at 322.748 kb on + strandat 322.749 kb on - strandat 322.749 kb on - strandat 322.797 kb on - strandat 322.807 kb on + strandat 322.808 kb on - strandat 322.808 kb on - strandat 322.808 kb on - strandat 322.808 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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320,483 + -0.8
320,484 - -0.4
320,688 + GFF2265 0.20 -0.2
320,688 + GFF2265 0.20 +0.5
320,688 + GFF2265 0.20 -0.0
320,689 - GFF2265 0.20 +0.4
320,689 - GFF2265 0.20 -0.5
320,835 + GFF2265 0.37 +0.1
320,835 + GFF2265 0.37 +0.0
320,910 + GFF2265 0.46 -0.1
320,910 + GFF2265 0.46 +0.5
320,910 + GFF2265 0.46 +1.5
320,910 + GFF2265 0.46 +0.2
320,911 - GFF2265 0.47 -2.5
320,911 - GFF2265 0.47 -0.8
320,911 - GFF2265 0.47 -1.5
320,991 + GFF2265 0.56 +0.2
320,991 + GFF2265 0.56 -1.5
320,992 - GFF2265 0.56 -1.2
321,093 + GFF2265 0.68 +0.8
321,195 + GFF2265 0.81 -0.3
321,506 + GFF2266 0.24 -0.3
321,506 + GFF2266 0.24 -0.8
321,689 + GFF2266 0.54 +1.1
321,690 - GFF2266 0.54 -1.9
321,746 + GFF2266 0.63 -0.5
321,746 + GFF2266 0.63 +1.4
321,746 + GFF2266 0.63 -1.0
321,747 - GFF2266 0.64 -2.0
321,747 - GFF2266 0.64 -0.2
321,845 + GFF2266 0.80 -0.2
321,845 + GFF2266 0.80 -0.4
321,845 + GFF2266 0.80 -1.5
321,846 - GFF2266 0.80 -0.8
321,846 - GFF2266 0.80 -2.1
321,846 - GFF2266 0.80 +0.1
321,846 - GFF2266 0.80 -0.1
321,846 - GFF2266 0.80 +1.1
321,846 - GFF2266 0.80 +0.2
321,846 - GFF2266 0.80 -0.8
321,846 - GFF2266 0.80 +0.1
321,846 - GFF2266 0.80 +0.1
321,846 - GFF2266 0.80 -0.0
321,920 + +0.1
321,966 - -0.5
322,008 + -0.0
322,085 + -0.7
322,085 + +0.1
322,086 - +1.5
322,086 - -1.0
322,086 - -1.5
322,091 + -0.6
322,091 + +0.6
322,092 - -0.1
322,092 - -0.3
322,535 + GFF2267 0.63 +1.2
322,535 + GFF2267 0.63 -0.7
322,535 + GFF2267 0.63 -0.8
322,535 + GFF2267 0.63 -1.1
322,535 + GFF2267 0.63 -0.2
322,536 - GFF2267 0.63 +0.2
322,536 - GFF2267 0.63 -1.5
322,536 - GFF2267 0.63 +0.2
322,536 - GFF2267 0.63 -0.5
322,536 - GFF2267 0.63 -0.7
322,536 - GFF2267 0.63 -0.1
322,536 - GFF2267 0.63 +0.5
322,536 - GFF2267 0.63 +2.1
322,586 + GFF2267 0.70 -1.4
322,586 + GFF2267 0.70 +0.6
322,586 + GFF2267 0.70 +0.6
322,587 - GFF2267 0.70 +0.2
322,587 - GFF2267 0.70 -0.1
322,748 + -0.6
322,749 - -0.6
322,749 - +0.7
322,797 - -0.3
322,807 + +1.1
322,808 - -0.8
322,808 - +0.4
322,808 - +0.3
322,808 - -0.7

Or see this region's nucleotide sequence