Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2243 and GFF2244 overlap by 4 nucleotides GFF2244 and GFF2245 overlap by 4 nucleotides GFF2245 and GFF2246 are separated by 36 nucleotides GFF2246 and GFF2247 are separated by 10 nucleotides
GFF2243 - Ornithine cyclodeaminase (EC 4.3.1.12), at 300,828 to 301,787
GFF2243
GFF2244 - hypothetical protein, at 301,784 to 302,647
GFF2244
GFF2245 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 302,644 to 303,219
GFF2245
GFF2246 - cellular communication/signal transduction, at 303,256 to 303,612
GFF2246
GFF2247 - hypothetical protein, at 303,623 to 304,150
GFF2247
Position (kb) 301
302
303 Strain fitness (log2 ratio) -2
-1
0
1 at 301.522 kb on + strand, within GFF2243 at 301.522 kb on + strand, within GFF2243 at 301.522 kb on + strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 302.017 kb on - strand, within GFF2244 at 302.017 kb on - strand, within GFF2244 at 302.095 kb on - strand, within GFF2244 at 302.095 kb on - strand, within GFF2244 at 302.233 kb on - strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.329 kb on - strand, within GFF2244 at 302.329 kb on - strand, within GFF2244 at 302.554 kb on - strand, within GFF2244 at 302.885 kb on + strand, within GFF2245 at 302.886 kb on - strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.969 kb on + strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 303.132 kb on - strand, within GFF2245 at 303.203 kb on - strand at 303.203 kb on - strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.320 kb on + strand, within GFF2246 at 303.320 kb on + strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.327 kb on - strand, within GFF2246
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 301,522 + GFF2243 0.72 +0.1 301,522 + GFF2243 0.72 -0.9 301,522 + GFF2243 0.72 -0.5 301,523 - GFF2243 0.72 -0.1 301,523 - GFF2243 0.72 -2.1 301,523 - GFF2243 0.72 -0.6 302,017 - GFF2244 0.27 -0.0 302,017 - GFF2244 0.27 -0.7 302,095 - GFF2244 0.36 -0.2 302,095 - GFF2244 0.36 +1.0 302,233 - GFF2244 0.52 +0.3 302,328 + GFF2244 0.63 -1.5 302,328 + GFF2244 0.63 -0.8 302,328 + GFF2244 0.63 -0.3 302,328 + GFF2244 0.63 +0.9 302,329 - GFF2244 0.63 +0.8 302,329 - GFF2244 0.63 -0.3 302,554 - GFF2244 0.89 +0.0 302,885 + GFF2245 0.42 -1.2 302,886 - GFF2245 0.42 -1.0 302,891 + GFF2245 0.43 +0.6 302,891 + GFF2245 0.43 -0.1 302,969 + GFF2245 0.56 -1.0 302,970 - GFF2245 0.57 -1.0 302,970 - GFF2245 0.57 -0.4 303,132 - GFF2245 0.85 +0.1 303,203 - -0.8 303,203 - -0.3 303,206 + -0.1 303,206 + +0.7 303,206 + -0.5 303,320 + GFF2246 0.18 -1.8 303,320 + GFF2246 0.18 -0.1 303,321 - GFF2246 0.18 +0.0 303,321 - GFF2246 0.18 +0.7 303,326 + GFF2246 0.20 -0.7 303,326 + GFF2246 0.20 -0.1 303,327 - GFF2246 0.20 -0.3
Or see this region's nucleotide sequence