Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2157 and GFF2158 are separated by 8 nucleotides GFF2158 and GFF2159 overlap by 4 nucleotides GFF2159 and GFF2160 overlap by 1 nucleotides GFF2160 and GFF2161 overlap by 4 nucleotides
GFF2157 - UPF0301 protein YqgE, at 210,912 to 211,511
GFF2157
GFF2158 - Putative pre-16S rRNA nuclease YqgF, at 211,520 to 211,969
GFF2158
GFF2159 - Pyrimidine operon regulatory protein PyrR, at 211,966 to 212,463
GFF2159
GFF2160 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 212,463 to 213,425
GFF2160
GFF2161 - Dihydroorotase (EC 3.5.2.3), at 213,422 to 214,711
GFF2161
Position (kb) 212
213
214 Strain fitness (log2 ratio) -2
-1
0
1
2 at 211.996 kb on + strand at 211.996 kb on + strand at 211.996 kb on + strand at 211.997 kb on - strand at 211.997 kb on - strand at 212.018 kb on + strand, within GFF2159 at 212.019 kb on - strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.838 kb on + strand, within GFF2160 at 212.838 kb on + strand, within GFF2160 at 212.839 kb on - strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.946 kb on + strand, within GFF2160 at 212.946 kb on + strand, within GFF2160 at 212.947 kb on - strand, within GFF2160 at 213.413 kb on + strand at 213.414 kb on - strand at 213.632 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.933 kb on - strand, within GFF2161 at 213.933 kb on - strand, within GFF2161 at 213.951 kb on - strand, within GFF2161 at 214.236 kb on - strand, within GFF2161
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 211,996 + +0.7 211,996 + -1.4 211,996 + -0.4 211,997 - +0.8 211,997 - -0.3 212,018 + GFF2159 0.10 +0.7 212,019 - GFF2159 0.11 +1.3 212,245 + GFF2159 0.56 -0.2 212,245 + GFF2159 0.56 -0.4 212,245 + GFF2159 0.56 -0.0 212,245 + GFF2159 0.56 -0.2 212,246 - GFF2159 0.56 -0.6 212,246 - GFF2159 0.56 -0.3 212,246 - GFF2159 0.56 +0.0 212,246 - GFF2159 0.56 -1.2 212,246 - GFF2159 0.56 +0.1 212,461 + +0.2 212,461 + -1.4 212,461 + +0.6 212,461 + -0.7 212,461 + +0.8 212,461 + -1.1 212,461 + -0.1 212,461 + -1.5 212,461 + +0.3 212,461 + -1.0 212,462 - -0.4 212,462 - -1.3 212,462 - -0.4 212,462 - +0.1 212,462 - -0.3 212,462 - +1.2 212,462 - -0.5 212,462 - +0.2 212,462 - +0.0 212,462 - +0.3 212,462 - +0.2 212,462 - -0.1 212,469 + +0.2 212,469 + -1.5 212,469 + +1.1 212,469 + -0.3 212,469 + +0.5 212,470 - +0.2 212,470 - +0.2 212,470 - +0.8 212,470 - -0.3 212,838 + GFF2160 0.39 +1.2 212,838 + GFF2160 0.39 -0.2 212,839 - GFF2160 0.39 -0.5 212,931 + GFF2160 0.49 +0.5 212,931 + GFF2160 0.49 +0.8 212,931 + GFF2160 0.49 +0.4 212,931 + GFF2160 0.49 +0.3 212,931 + GFF2160 0.49 +1.8 212,931 + GFF2160 0.49 -2.2 212,931 + GFF2160 0.49 +0.1 212,932 - GFF2160 0.49 -0.0 212,932 - GFF2160 0.49 -0.2 212,932 - GFF2160 0.49 +0.4 212,932 - GFF2160 0.49 -1.5 212,932 - GFF2160 0.49 -0.3 212,932 - GFF2160 0.49 -0.3 212,932 - GFF2160 0.49 +0.5 212,932 - GFF2160 0.49 +0.4 212,932 - GFF2160 0.49 -0.7 212,932 - GFF2160 0.49 +1.3 212,932 - GFF2160 0.49 -0.2 212,932 - GFF2160 0.49 +0.4 212,932 - GFF2160 0.49 -0.4 212,946 + GFF2160 0.50 -0.9 212,946 + GFF2160 0.50 +0.8 212,947 - GFF2160 0.50 +0.6 213,413 + -1.0 213,414 - -0.8 213,632 + GFF2161 0.16 +1.8 213,932 + GFF2161 0.40 -0.5 213,932 + GFF2161 0.40 -0.8 213,932 + GFF2161 0.40 -1.0 213,933 - GFF2161 0.40 +0.8 213,933 - GFF2161 0.40 -0.6 213,951 - GFF2161 0.41 -0.2 214,236 - GFF2161 0.63 +0.2
Or see this region's nucleotide sequence