Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2146 and GFF2147 are separated by 90 nucleotides GFF2147 and GFF2148 are separated by 3 nucleotides
GFF2146 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 194,477 to 196,084
GFF2146
GFF2147 - no description, at 196,175 to 196,690
GFF2147
GFF2148 - Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8), at 196,694 to 197,974
GFF2148
Position (kb) 196
197 Strain fitness (log2 ratio) -2
-1
0
1 at 196.157 kb on - strand at 196.176 kb on + strand at 196.176 kb on + strand at 196.197 kb on + strand at 196.197 kb on + strand at 196.251 kb on + strand, within GFF2147 at 196.251 kb on + strand, within GFF2147 at 196.252 kb on - strand, within GFF2147 at 196.252 kb on - strand, within GFF2147 at 196.329 kb on + strand, within GFF2147 at 196.330 kb on - strand, within GFF2147 at 196.330 kb on - strand, within GFF2147 at 196.339 kb on - strand, within GFF2147 at 196.380 kb on + strand, within GFF2147 at 196.381 kb on - strand, within GFF2147 at 196.381 kb on - strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.414 kb on - strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.482 kb on + strand, within GFF2147 at 196.545 kb on + strand, within GFF2147 at 196.546 kb on - strand, within GFF2147 at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.696 kb on - strand at 196.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 196,157 - +0.1 196,176 + -1.2 196,176 + -0.6 196,197 + +0.4 196,197 + -0.1 196,251 + GFF2147 0.15 -1.9 196,251 + GFF2147 0.15 +0.4 196,252 - GFF2147 0.15 -0.7 196,252 - GFF2147 0.15 -0.5 196,329 + GFF2147 0.30 +0.2 196,330 - GFF2147 0.30 -0.6 196,330 - GFF2147 0.30 -1.4 196,339 - GFF2147 0.32 -0.6 196,380 + GFF2147 0.40 -0.3 196,381 - GFF2147 0.40 +1.1 196,381 - GFF2147 0.40 -0.6 196,397 + GFF2147 0.43 -0.0 196,397 + GFF2147 0.43 -0.9 196,397 + GFF2147 0.43 -0.2 196,397 + GFF2147 0.43 -0.2 196,397 + GFF2147 0.43 +0.0 196,397 + GFF2147 0.43 -0.5 196,397 + GFF2147 0.43 -0.1 196,397 + GFF2147 0.43 -0.2 196,398 - GFF2147 0.43 +0.8 196,398 - GFF2147 0.43 +0.5 196,398 - GFF2147 0.43 -1.5 196,398 - GFF2147 0.43 -0.8 196,398 - GFF2147 0.43 +0.1 196,398 - GFF2147 0.43 +0.9 196,401 + GFF2147 0.44 -0.9 196,401 + GFF2147 0.44 -0.9 196,401 + GFF2147 0.44 +0.0 196,401 + GFF2147 0.44 +0.5 196,401 + GFF2147 0.44 -0.5 196,401 + GFF2147 0.44 +0.4 196,401 + GFF2147 0.44 -0.5 196,401 + GFF2147 0.44 -1.0 196,401 + GFF2147 0.44 -1.5 196,401 + GFF2147 0.44 -0.9 196,401 + GFF2147 0.44 -1.2 196,401 + GFF2147 0.44 -0.0 196,401 + GFF2147 0.44 +0.8 196,402 - GFF2147 0.44 -0.3 196,402 - GFF2147 0.44 +0.7 196,402 - GFF2147 0.44 +1.0 196,402 - GFF2147 0.44 -0.9 196,402 - GFF2147 0.44 +0.7 196,402 - GFF2147 0.44 -0.3 196,402 - GFF2147 0.44 -0.2 196,402 - GFF2147 0.44 -0.7 196,402 - GFF2147 0.44 -1.3 196,402 - GFF2147 0.44 +0.0 196,402 - GFF2147 0.44 -1.3 196,402 - GFF2147 0.44 -2.1 196,402 - GFF2147 0.44 -1.1 196,402 - GFF2147 0.44 -0.8 196,402 - GFF2147 0.44 -0.4 196,413 + GFF2147 0.46 -0.3 196,413 + GFF2147 0.46 -1.2 196,413 + GFF2147 0.46 -0.6 196,413 + GFF2147 0.46 -0.4 196,413 + GFF2147 0.46 +0.8 196,414 - GFF2147 0.46 +0.3 196,464 + GFF2147 0.56 -0.3 196,464 + GFF2147 0.56 -0.5 196,464 + GFF2147 0.56 -1.5 196,465 - GFF2147 0.56 +0.2 196,465 - GFF2147 0.56 -1.8 196,465 - GFF2147 0.56 -0.6 196,482 + GFF2147 0.59 -0.6 196,545 + GFF2147 0.72 -0.1 196,546 - GFF2147 0.72 +0.1 196,695 + -0.2 196,695 + -1.0 196,695 + -2.2 196,695 + +0.6 196,695 + -0.8 196,696 - -0.5 196,706 + +1.1
Or see this region's nucleotide sequence