Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2139 and GFF2140 are separated by 52 nucleotides GFF2140 and GFF2141 are separated by 208 nucleotides
GFF2139 - Ribose ABC transporter, ATP-binding protein RbsA (TC 3.A.1.2.1), at 187,706 to 189,334
GFF2139
GFF2140 - Ribose ABC transporter, substrate-binding protein RbsB (TC 3.A.1.2.1), at 189,387 to 190,340
GFF2140
GFF2141 - GTP-binding and nucleic acid-binding protein YchF, at 190,549 to 191,640
GFF2141
Position (kb) 189
190
191 Strain fitness (log2 ratio) -2
-1
0
1 at 188.736 kb on + strand, within GFF2139 at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.388 kb on + strand at 189.580 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.714 kb on - strand, within GFF2140 at 189.791 kb on - strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.322 kb on + strand at 190.402 kb on + strand at 190.403 kb on - strand at 190.405 kb on + strand at 190.406 kb on - strand at 190.447 kb on + strand at 190.448 kb on - strand at 190.590 kb on + strand at 190.591 kb on - strand at 190.591 kb on - strand at 190.604 kb on + strand at 191.200 kb on + strand, within GFF2141 at 191.200 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 188,736 + GFF2139 0.63 -0.3 189,279 + -0.8 189,279 + -1.7 189,279 + -0.5 189,279 + +0.8 189,279 + -0.2 189,388 + -2.0 189,580 + GFF2140 0.20 -2.2 189,713 + GFF2140 0.34 -1.0 189,713 + GFF2140 0.34 +0.6 189,714 - GFF2140 0.34 -0.2 189,791 - GFF2140 0.42 +0.0 190,153 + GFF2140 0.80 -0.6 190,153 + GFF2140 0.80 -0.1 190,153 + GFF2140 0.80 -0.2 190,154 - GFF2140 0.80 -1.1 190,154 - GFF2140 0.80 +0.2 190,322 + -0.6 190,402 + -1.2 190,403 - -0.7 190,405 + -0.9 190,406 - +1.1 190,447 + -0.2 190,448 - -1.0 190,590 + -0.6 190,591 - +0.0 190,591 - -0.4 190,604 + -0.5 191,200 + GFF2141 0.60 -2.0 191,200 + GFF2141 0.60 -0.4 191,221 + GFF2141 0.62 -0.4 191,221 + GFF2141 0.62 +0.5 191,221 + GFF2141 0.62 -0.4
Or see this region's nucleotide sequence