Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2138 and GFF2139 overlap by 4 nucleotides GFF2139 and GFF2140 are separated by 52 nucleotides
GFF2138 - Ribose ABC transporter, permease protein RbsC (TC 3.A.1.2.1), at 186,720 to 187,709
GFF2138
GFF2139 - Ribose ABC transporter, ATP-binding protein RbsA (TC 3.A.1.2.1), at 187,706 to 189,334
GFF2139
GFF2140 - Ribose ABC transporter, substrate-binding protein RbsB (TC 3.A.1.2.1), at 189,387 to 190,340
GFF2140
Position (kb) 187
188
189
190 Strain fitness (log2 ratio) -2
-1
0
1 at 187.270 kb on + strand, within GFF2138 at 187.270 kb on + strand, within GFF2138 at 187.271 kb on - strand, within GFF2138 at 187.582 kb on + strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.694 kb on + strand at 187.998 kb on + strand, within GFF2139 at 188.090 kb on + strand, within GFF2139 at 188.736 kb on + strand, within GFF2139 at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.279 kb on + strand at 189.388 kb on + strand at 189.580 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.714 kb on - strand, within GFF2140 at 189.791 kb on - strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.322 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 187,270 + GFF2138 0.56 +0.2 187,270 + GFF2138 0.56 +0.8 187,271 - GFF2138 0.56 -0.8 187,582 + GFF2138 0.87 -0.3 187,583 - GFF2138 0.87 +0.6 187,583 - GFF2138 0.87 -0.8 187,642 + -2.3 187,642 + -0.9 187,642 + -1.6 187,642 + +0.3 187,642 + +0.6 187,642 + -1.1 187,643 - -0.4 187,643 - -0.2 187,643 - +0.1 187,643 - +0.5 187,643 - -0.1 187,643 - -1.1 187,694 + -0.7 187,998 + GFF2139 0.18 -1.4 188,090 + GFF2139 0.24 -1.7 188,736 + GFF2139 0.63 -0.3 189,279 + -0.8 189,279 + -1.7 189,279 + -0.5 189,279 + +0.8 189,279 + -0.2 189,388 + -2.0 189,580 + GFF2140 0.20 -2.2 189,713 + GFF2140 0.34 -1.0 189,713 + GFF2140 0.34 +0.6 189,714 - GFF2140 0.34 -0.2 189,791 - GFF2140 0.42 +0.0 190,153 + GFF2140 0.80 -0.6 190,153 + GFF2140 0.80 -0.1 190,153 + GFF2140 0.80 -0.2 190,154 - GFF2140 0.80 -1.1 190,154 - GFF2140 0.80 +0.2 190,322 + -0.6
Or see this region's nucleotide sequence