Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2116 and GFF2117 are separated by 223 nucleotides GFF2117 and GFF2118 overlap by 4 nucleotides GFF2118 and GFF2119 overlap by 4 nucleotides GFF2119 and GFF2120 overlap by 4 nucleotides
GFF2116 - Cytochrome P450, at 165,737 to 167,131
GFF2116
GFF2117 - KEGG: type IV fimbrial biogenesis protein FimT, at 167,355 to 167,906
GFF2117
GFF2118 - hypothetical protein, at 167,903 to 168,460
GFF2118
GFF2119 - Type IV fimbrial biogenesis protein PilW, at 168,457 to 169,221
GFF2119
GFF2120 - Type IV fimbrial biogenesis protein PilX, at 169,218 to 169,799
GFF2120
Position (kb) 167
168
169 Strain fitness (log2 ratio) -2
-1
0
1
2 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.926 kb on - strand, within GFF2116 at 166.926 kb on - strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.928 kb on - strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.954 kb on - strand, within GFF2116 at 167.254 kb on + strand at 167.274 kb on + strand at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.497 kb on - strand, within GFF2117 at 167.517 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 168.028 kb on + strand, within GFF2118 at 168.245 kb on + strand, within GFF2118 at 168.290 kb on - strand, within GFF2118 at 168.293 kb on + strand, within GFF2118 at 168.371 kb on + strand, within GFF2118 at 168.372 kb on - strand, within GFF2118 at 168.447 kb on - strand at 168.829 kb on + strand, within GFF2119 at 168.829 kb on + strand, within GFF2119 at 168.915 kb on + strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.235 kb on + strand at 169.338 kb on + strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 166,925 + GFF2116 0.85 +0.7 166,925 + GFF2116 0.85 -0.6 166,925 + GFF2116 0.85 -1.4 166,925 + GFF2116 0.85 -0.2 166,925 + GFF2116 0.85 -1.1 166,925 + GFF2116 0.85 -0.5 166,925 + GFF2116 0.85 +0.6 166,925 + GFF2116 0.85 -0.1 166,925 + GFF2116 0.85 +1.0 166,925 + GFF2116 0.85 +1.0 166,926 - GFF2116 0.85 +0.6 166,926 - GFF2116 0.85 -0.3 166,927 + GFF2116 0.85 -0.2 166,927 + GFF2116 0.85 +0.0 166,927 + GFF2116 0.85 -0.5 166,928 - GFF2116 0.85 -0.7 166,953 + GFF2116 0.87 +0.8 166,953 + GFF2116 0.87 -0.2 166,953 + GFF2116 0.87 -0.2 166,953 + GFF2116 0.87 +0.2 166,953 + GFF2116 0.87 -1.9 166,954 - GFF2116 0.87 +1.8 167,254 + +0.8 167,274 + -0.8 167,464 + GFF2117 0.20 -0.3 167,464 + GFF2117 0.20 -0.2 167,464 + GFF2117 0.20 -1.1 167,497 - GFF2117 0.26 +1.5 167,517 + GFF2117 0.29 -0.5 167,742 + GFF2117 0.70 -2.7 167,742 + GFF2117 0.70 -0.1 167,743 - GFF2117 0.70 +1.1 167,743 - GFF2117 0.70 -0.2 167,743 - GFF2117 0.70 +1.5 167,743 - GFF2117 0.70 -0.2 168,028 + GFF2118 0.22 -0.9 168,245 + GFF2118 0.61 +1.1 168,290 - GFF2118 0.69 -0.3 168,293 + GFF2118 0.70 -0.5 168,371 + GFF2118 0.84 -2.4 168,372 - GFF2118 0.84 -0.6 168,447 - -1.9 168,829 + GFF2119 0.49 +0.5 168,829 + GFF2119 0.49 +0.8 168,915 + GFF2119 0.60 -0.3 168,916 - GFF2119 0.60 -1.2 168,916 - GFF2119 0.60 +0.2 168,916 - GFF2119 0.60 +0.8 169,027 + GFF2119 0.75 -0.5 169,027 + GFF2119 0.75 +0.4 169,027 + GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 -0.5 169,028 - GFF2119 0.75 +1.1 169,028 - GFF2119 0.75 -0.5 169,028 - GFF2119 0.75 -0.8 169,028 - GFF2119 0.75 -0.4 169,028 - GFF2119 0.75 -0.0 169,028 - GFF2119 0.75 -0.0 169,235 + -1.1 169,338 + GFF2120 0.21 -0.7 169,339 - GFF2120 0.21 -0.4 169,339 - GFF2120 0.21 +0.4 169,339 - GFF2120 0.21 -0.3 169,339 - GFF2120 0.21 +2.1
Or see this region's nucleotide sequence