Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2013 and GFF2014 are separated by 58 nucleotides GFF2014 and GFF2015 are separated by 44 nucleotides GFF2015 and GFF2016 are separated by 6 nucleotides
GFF2013 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 57,269 to 59,272
GFF2013
GFF2014 - hypothetical protein, at 59,331 to 60,296
GFF2014
GFF2015 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 60,341 to 60,961
GFF2015
GFF2016 - hypothetical protein, at 60,968 to 61,282
GFF2016
Position (kb) 59
60
61 Strain fitness (log2 ratio) -2
-1
0
1
2 at 58.352 kb on + strand, within GFF2013 at 58.353 kb on - strand, within GFF2013 at 58.353 kb on - strand, within GFF2013 at 58.440 kb on - strand, within GFF2013 at 58.440 kb on - strand, within GFF2013 at 58.440 kb on - strand, within GFF2013 at 58.769 kb on + strand, within GFF2013 at 58.826 kb on + strand, within GFF2013 at 58.826 kb on + strand, within GFF2013 at 58.827 kb on - strand, within GFF2013 at 58.827 kb on - strand, within GFF2013 at 58.827 kb on - strand, within GFF2013 at 59.295 kb on + strand at 59.508 kb on + strand, within GFF2014 at 59.508 kb on + strand, within GFF2014 at 59.883 kb on + strand, within GFF2014 at 60.126 kb on + strand, within GFF2014 at 60.127 kb on - strand, within GFF2014 at 60.303 kb on + strand at 60.304 kb on - strand at 60.304 kb on - strand at 60.362 kb on + strand at 60.362 kb on + strand at 60.389 kb on + strand at 60.390 kb on - strand at 60.390 kb on - strand at 60.390 kb on - strand at 60.569 kb on + strand, within GFF2015 at 60.569 kb on + strand, within GFF2015 at 60.569 kb on + strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.570 kb on - strand, within GFF2015 at 60.902 kb on + strand at 60.902 kb on + strand at 60.902 kb on + strand at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.017 kb on + strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.018 kb on - strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.113 kb on + strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.114 kb on - strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.164 kb on + strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.165 kb on - strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.221 kb on + strand, within GFF2016 at 61.222 kb on - strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.224 kb on + strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.225 kb on - strand, within GFF2016 at 61.254 kb on + strand at 61.255 kb on - strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.260 kb on + strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand at 61.261 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 58,352 + GFF2013 0.54 -0.2 58,353 - GFF2013 0.54 -1.2 58,353 - GFF2013 0.54 -0.2 58,440 - GFF2013 0.58 -0.5 58,440 - GFF2013 0.58 +0.3 58,440 - GFF2013 0.58 -0.8 58,769 + GFF2013 0.75 -0.3 58,826 + GFF2013 0.78 +0.1 58,826 + GFF2013 0.78 +0.2 58,827 - GFF2013 0.78 -0.5 58,827 - GFF2013 0.78 +0.3 58,827 - GFF2013 0.78 -0.0 59,295 + -0.5 59,508 + GFF2014 0.18 -0.4 59,508 + GFF2014 0.18 -0.9 59,883 + GFF2014 0.57 +0.9 60,126 + GFF2014 0.82 +0.2 60,127 - GFF2014 0.82 +0.4 60,303 + +0.2 60,304 - -0.0 60,304 - -1.2 60,362 + +0.3 60,362 + -0.3 60,389 + -1.5 60,390 - -0.2 60,390 - +0.6 60,390 - -2.6 60,569 + GFF2015 0.37 -0.3 60,569 + GFF2015 0.37 -0.5 60,569 + GFF2015 0.37 -0.4 60,570 - GFF2015 0.37 -0.3 60,570 - GFF2015 0.37 -0.4 60,570 - GFF2015 0.37 -2.0 60,902 + -1.0 60,902 + +0.3 60,902 + -0.4 61,017 + GFF2016 0.16 +1.0 61,017 + GFF2016 0.16 +0.2 61,017 + GFF2016 0.16 +0.7 61,017 + GFF2016 0.16 +0.0 61,018 - GFF2016 0.16 -0.7 61,018 - GFF2016 0.16 -2.0 61,018 - GFF2016 0.16 -0.5 61,018 - GFF2016 0.16 -1.5 61,018 - GFF2016 0.16 -1.4 61,113 + GFF2016 0.46 +0.6 61,113 + GFF2016 0.46 -1.3 61,113 + GFF2016 0.46 +0.1 61,113 + GFF2016 0.46 +0.3 61,113 + GFF2016 0.46 -2.3 61,113 + GFF2016 0.46 -1.4 61,113 + GFF2016 0.46 -1.2 61,113 + GFF2016 0.46 -1.6 61,113 + GFF2016 0.46 +0.4 61,114 - GFF2016 0.46 -0.5 61,114 - GFF2016 0.46 +0.1 61,114 - GFF2016 0.46 +1.2 61,114 - GFF2016 0.46 +1.2 61,114 - GFF2016 0.46 +1.1 61,114 - GFF2016 0.46 +0.6 61,114 - GFF2016 0.46 -1.9 61,114 - GFF2016 0.46 +0.9 61,164 + GFF2016 0.62 -1.1 61,164 + GFF2016 0.62 -0.9 61,164 + GFF2016 0.62 -2.4 61,164 + GFF2016 0.62 +0.2 61,164 + GFF2016 0.62 +0.4 61,164 + GFF2016 0.62 -0.9 61,164 + GFF2016 0.62 -0.5 61,165 - GFF2016 0.63 +1.0 61,165 - GFF2016 0.63 -0.2 61,165 - GFF2016 0.63 -0.8 61,165 - GFF2016 0.63 -0.7 61,165 - GFF2016 0.63 -0.9 61,165 - GFF2016 0.63 -0.8 61,221 + GFF2016 0.80 +0.0 61,221 + GFF2016 0.80 -0.4 61,221 + GFF2016 0.80 -1.0 61,222 - GFF2016 0.81 -0.3 61,224 + GFF2016 0.81 -0.8 61,224 + GFF2016 0.81 -0.5 61,224 + GFF2016 0.81 +1.4 61,224 + GFF2016 0.81 +0.8 61,225 - GFF2016 0.82 -0.4 61,225 - GFF2016 0.82 +2.4 61,225 - GFF2016 0.82 -1.2 61,225 - GFF2016 0.82 -0.2 61,225 - GFF2016 0.82 -0.8 61,225 - GFF2016 0.82 -0.0 61,225 - GFF2016 0.82 -1.6 61,254 + -0.2 61,255 - +0.1 61,260 + +0.2 61,260 + -0.4 61,260 + -1.9 61,260 + -1.3 61,260 + -0.0 61,260 + -0.5 61,261 - -2.0 61,261 - +0.8 61,261 - +0.9 61,261 - -0.5 61,261 - -0.1 61,261 - -0.3 61,261 - -0.7 61,261 - -0.8 61,261 - -0.2 61,261 - +0.1 61,261 - -1.3 61,261 - -0.2
Or see this region's nucleotide sequence