Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1680 and GFF1681 are separated by 39 nucleotides GFF1681 and GFF1682 are separated by 1 nucleotides GFF1682 and GFF1683 overlap by 4 nucleotides GFF1683 and GFF1684 overlap by 4 nucleotides
GFF1680 - KEGG: GntR family transcriptional regulator, at 325,105 to 325,986
GFF1680
GFF1681 - Methylisocitrate lyase (EC 4.1.3.30), at 326,026 to 326,898
GFF1681
GFF1682 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 326,900 to 328,300
GFF1682
GFF1683 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 328,297 to 328,929
GFF1683
GFF1684 - no description, at 328,926 to 329,564
GFF1684
Position (kb) 326
327
328
329 Strain fitness (log2 ratio) -1
0
1
2 at 326.092 kb on + strand at 326.144 kb on - strand, within GFF1681 at 326.282 kb on - strand, within GFF1681 at 326.698 kb on + strand, within GFF1681 at 326.699 kb on - strand, within GFF1681 at 327.489 kb on + strand, within GFF1682 at 327.872 kb on + strand, within GFF1682 at 327.872 kb on + strand, within GFF1682 at 327.873 kb on - strand, within GFF1682 at 328.423 kb on + strand, within GFF1683
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 326,092 + -0.9 326,144 - GFF1681 0.14 -0.7 326,282 - GFF1681 0.29 +0.1 326,698 + GFF1681 0.77 -0.5 326,699 - GFF1681 0.77 +1.8 327,489 + GFF1682 0.42 -1.7 327,872 + GFF1682 0.69 +0.2 327,872 + GFF1682 0.69 +0.1 327,873 - GFF1682 0.69 +0.6 328,423 + GFF1683 0.20 -0.2
Or see this region's nucleotide sequence