Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1614 and GFF1615 overlap by 4 nucleotides GFF1615 and GFF1616 are separated by 128 nucleotides GFF1616 and GFF1617 are separated by 15 nucleotides GFF1617 and GFF1618 are separated by 99 nucleotides
GFF1614 - Phosphonate ABC transporter ATP-binding protein PhnC (TC 3.A.1.9.1), at 211,983 to 212,783
GFF1614
GFF1615 - Phosphonate ABC transporter substrate-binding protein PhnD (TC 3.A.1.9.1), at 212,780 to 213,649
GFF1615
GFF1616 - Phosphonate uptake and metabolism regulator, LysR-family, at 213,778 to 214,665
GFF1616
GFF1617 - Phosphonate uptake and metabolism regulator, LysR-family, at 214,681 to 215,553
GFF1617
GFF1618 - Phospholipase C (EC 3.1.4.3), at 215,653 to 217,803
GFF1618
Position (kb) 213
214
215 Strain fitness (log2 ratio) -2
-1
0
1
2 at 212.838 kb on + strand at 212.839 kb on - strand at 212.839 kb on - strand at 213.300 kb on + strand, within GFF1615 at 213.375 kb on + strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.441 kb on + strand, within GFF1615 at 213.716 kb on + strand at 213.716 kb on + strand at 213.717 kb on - strand at 213.717 kb on - strand at 213.729 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.774 kb on - strand at 213.774 kb on - strand at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.013 kb on - strand, within GFF1616 at 214.013 kb on - strand, within GFF1616 at 214.183 kb on + strand, within GFF1616 at 214.246 kb on + strand, within GFF1616 at 214.564 kb on + strand, within GFF1616 at 214.565 kb on - strand, within GFF1616 at 214.565 kb on - strand, within GFF1616 at 214.571 kb on - strand, within GFF1616 at 214.647 kb on - strand at 214.829 kb on + strand, within GFF1617 at 214.829 kb on + strand, within GFF1617 at 214.829 kb on + strand, within GFF1617 at 215.084 kb on + strand, within GFF1617 at 215.084 kb on + strand, within GFF1617 at 215.085 kb on - strand, within GFF1617 at 215.147 kb on + strand, within GFF1617 at 215.148 kb on - strand, within GFF1617 at 215.225 kb on + strand, within GFF1617 at 215.409 kb on - strand, within GFF1617 at 215.446 kb on + strand, within GFF1617 at 215.446 kb on + strand, within GFF1617 at 215.446 kb on + strand, within GFF1617 at 215.447 kb on - strand, within GFF1617 at 215.447 kb on - strand, within GFF1617 at 215.447 kb on - strand, within GFF1617 at 215.536 kb on + strand at 215.537 kb on - strand at 215.537 kb on - strand at 215.629 kb on + strand at 215.629 kb on + strand at 215.629 kb on + strand at 215.630 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 212,838 + +0.3 212,839 - -0.3 212,839 - -0.9 213,300 + GFF1615 0.60 +0.5 213,375 + GFF1615 0.68 +0.6 213,376 - GFF1615 0.69 -0.3 213,376 - GFF1615 0.69 -0.1 213,376 - GFF1615 0.69 -0.6 213,376 - GFF1615 0.69 +0.2 213,376 - GFF1615 0.69 -0.6 213,441 + GFF1615 0.76 -0.0 213,716 + +1.3 213,716 + -2.3 213,717 - -1.0 213,717 - -0.8 213,729 + +0.2 213,773 + +0.4 213,773 + -0.3 213,773 + -1.8 213,773 + -0.0 213,774 - +0.2 213,774 - +0.8 214,012 + GFF1616 0.26 -1.2 214,012 + GFF1616 0.26 +0.2 214,012 + GFF1616 0.26 +0.1 214,012 + GFF1616 0.26 -1.8 214,013 - GFF1616 0.26 +0.4 214,013 - GFF1616 0.26 -0.4 214,183 + GFF1616 0.46 +0.3 214,246 + GFF1616 0.53 -1.8 214,564 + GFF1616 0.89 +0.2 214,565 - GFF1616 0.89 -0.4 214,565 - GFF1616 0.89 +0.1 214,571 - GFF1616 0.89 -0.6 214,647 - +1.8 214,829 + GFF1617 0.17 -0.6 214,829 + GFF1617 0.17 -0.1 214,829 + GFF1617 0.17 -0.1 215,084 + GFF1617 0.46 -0.5 215,084 + GFF1617 0.46 -0.8 215,085 - GFF1617 0.46 -0.0 215,147 + GFF1617 0.53 +0.6 215,148 - GFF1617 0.53 -0.8 215,225 + GFF1617 0.62 -0.3 215,409 - GFF1617 0.83 -0.2 215,446 + GFF1617 0.88 +0.2 215,446 + GFF1617 0.88 -0.2 215,446 + GFF1617 0.88 -0.3 215,447 - GFF1617 0.88 -0.5 215,447 - GFF1617 0.88 +0.8 215,447 - GFF1617 0.88 -0.0 215,536 + +0.0 215,537 - -0.9 215,537 - -0.3 215,629 + -1.0 215,629 + -0.9 215,629 + -0.0 215,630 - -0.7
Or see this region's nucleotide sequence