Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1535 and GFF1536 overlap by 4 nucleotides GFF1536 and GFF1537 are separated by 10 nucleotides GFF1537 and GFF1538 are separated by 3 nucleotides GFF1538 and GFF1539 are separated by 256 nucleotides
GFF1535 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 133,012 to 133,917
GFF1535
GFF1536 - BUG/TctC family periplasmic protein, at 133,914 to 134,897
GFF1536
GFF1537 - FIG01127817: hypothetical protein, at 134,908 to 136,569
GFF1537
GFF1538 - hypothetical protein, at 136,573 to 137,265
GFF1538
GFF1539 - RsbT co-antagonist protein RsbRA, at 137,522 to 138,367
GFF1539
Position (kb) 134
135
136
137 Strain fitness (log2 ratio) -2
-1
0
1 at 134.001 kb on + strand at 134.233 kb on - strand, within GFF1536 at 134.248 kb on - strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.386 kb on - strand, within GFF1536 at 134.475 kb on + strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.857 kb on - strand at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.179 kb on - strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.602 kb on - strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.832 kb on + strand, within GFF1537 at 135.833 kb on - strand, within GFF1537 at 135.875 kb on - strand, within GFF1537 at 136.069 kb on + strand, within GFF1537 at 136.069 kb on + strand, within GFF1537 at 136.070 kb on - strand, within GFF1537 at 136.070 kb on - strand, within GFF1537 at 136.070 kb on - strand, within GFF1537 at 136.070 kb on - strand, within GFF1537 at 136.638 kb on - strand at 136.638 kb on - strand at 136.638 kb on - strand at 137.081 kb on + strand, within GFF1538 at 137.082 kb on - strand, within GFF1538 at 137.082 kb on - strand, within GFF1538 at 137.082 kb on - strand, within GFF1538 at 137.082 kb on - strand, within GFF1538 at 137.276 kb on + strand at 137.276 kb on + strand at 137.276 kb on + strand at 137.277 kb on - strand at 137.332 kb on - strand at 137.332 kb on - strand at 137.342 kb on + strand at 137.354 kb on + strand at 137.354 kb on + strand at 137.354 kb on + strand at 137.354 kb on + strand at 137.355 kb on - strand at 137.412 kb on + strand at 137.412 kb on + strand at 137.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 134,001 + -1.6 134,233 - GFF1536 0.32 -1.3 134,248 - GFF1536 0.34 -0.3 134,385 + GFF1536 0.48 -1.8 134,385 + GFF1536 0.48 -0.8 134,385 + GFF1536 0.48 +0.2 134,385 + GFF1536 0.48 -0.8 134,386 - GFF1536 0.48 -0.4 134,475 + GFF1536 0.57 +1.7 134,476 - GFF1536 0.57 -1.4 134,476 - GFF1536 0.57 +0.2 134,857 - -0.3 135,145 + GFF1537 0.14 -0.1 135,145 + GFF1537 0.14 -1.1 135,145 + GFF1537 0.14 -0.5 135,146 - GFF1537 0.14 -0.2 135,146 - GFF1537 0.14 -1.2 135,146 - GFF1537 0.14 -0.3 135,175 + GFF1537 0.16 -0.6 135,175 + GFF1537 0.16 +0.2 135,176 - GFF1537 0.16 +0.7 135,176 - GFF1537 0.16 -0.7 135,178 + GFF1537 0.16 -0.4 135,178 + GFF1537 0.16 -0.7 135,179 - GFF1537 0.16 +0.7 135,601 + GFF1537 0.42 +0.6 135,601 + GFF1537 0.42 -0.1 135,602 - GFF1537 0.42 -0.6 135,775 + GFF1537 0.52 -0.5 135,775 + GFF1537 0.52 -0.8 135,775 + GFF1537 0.52 -0.3 135,775 + GFF1537 0.52 -0.1 135,775 + GFF1537 0.52 +0.0 135,776 - GFF1537 0.52 -1.1 135,776 - GFF1537 0.52 -1.7 135,776 - GFF1537 0.52 -1.1 135,832 + GFF1537 0.56 +1.0 135,833 - GFF1537 0.56 -0.5 135,875 - GFF1537 0.58 +0.9 136,069 + GFF1537 0.70 -1.5 136,069 + GFF1537 0.70 -0.4 136,070 - GFF1537 0.70 -2.1 136,070 - GFF1537 0.70 -0.1 136,070 - GFF1537 0.70 -0.3 136,070 - GFF1537 0.70 -1.6 136,638 - -0.8 136,638 - +0.5 136,638 - -0.2 137,081 + GFF1538 0.73 -1.6 137,082 - GFF1538 0.73 -0.0 137,082 - GFF1538 0.73 -0.7 137,082 - GFF1538 0.73 -0.6 137,082 - GFF1538 0.73 +0.6 137,276 + -1.8 137,276 + -1.7 137,276 + -0.8 137,277 - -0.6 137,332 - -1.1 137,332 - -0.3 137,342 + +0.1 137,354 + -0.2 137,354 + -0.7 137,354 + +0.2 137,354 + +0.9 137,355 - -0.1 137,412 + +1.4 137,412 + -2.5 137,413 - +1.7
Or see this region's nucleotide sequence