Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1340 and GFF1341 are separated by 1 nucleotides GFF1341 and GFF1342 are separated by 150 nucleotides GFF1342 and GFF1343 are separated by 31 nucleotides
GFF1340 - ABC transporter, ATP-binding protein 1 RSc1753, at 682,232 to 683,002
GFF1340
GFF1341 - ABC transporter, ATP-binding protein 2 RSc1754, at 683,004 to 683,699
GFF1341
GFF1342 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 683,850 to 685,955
GFF1342
GFF1343 - macromolecule metabolism; macromolecule degradation; degradation of proteins, peptides, glycopeptides, at 685,987 to 687,462
GFF1343
Position (kb)
683
684
685
686 Strain fitness (log2 ratio)
-2
-1
0
1 at 682.892 kb on + strand, within GFF1340 at 682.958 kb on + strand at 682.958 kb on + strand at 682.959 kb on - strand at 682.959 kb on - strand at 683.031 kb on + strand at 683.032 kb on - strand at 683.035 kb on - strand at 683.211 kb on + strand, within GFF1341 at 683.212 kb on - strand, within GFF1341 at 683.212 kb on - strand, within GFF1341 at 683.239 kb on - strand, within GFF1341 at 683.239 kb on - strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.346 kb on + strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.347 kb on - strand, within GFF1341 at 683.520 kb on + strand, within GFF1341 at 683.521 kb on - strand, within GFF1341 at 683.701 kb on - strand at 683.743 kb on + strand at 683.744 kb on - strand at 683.744 kb on - strand at 683.784 kb on - strand at 683.862 kb on + strand at 683.862 kb on + strand at 683.863 kb on - strand at 683.863 kb on - strand at 683.863 kb on - strand at 684.184 kb on - strand, within GFF1342 at 684.502 kb on - strand, within GFF1342 at 684.502 kb on - strand, within GFF1342 at 684.502 kb on - strand, within GFF1342 at 684.546 kb on + strand, within GFF1342 at 684.885 kb on + strand, within GFF1342 at 684.885 kb on + strand, within GFF1342 at 684.885 kb on + strand, within GFF1342 at 684.886 kb on - strand, within GFF1342 at 684.886 kb on - strand, within GFF1342 at 684.927 kb on + strand, within GFF1342 at 684.927 kb on + strand, within GFF1342 at 684.927 kb on + strand, within GFF1342 at 684.927 kb on + strand, within GFF1342 at 684.927 kb on + strand, within GFF1342 at 685.323 kb on + strand, within GFF1342 at 685.323 kb on + strand, within GFF1342 at 685.323 kb on + strand, within GFF1342 at 685.323 kb on + strand, within GFF1342 at 685.323 kb on + strand, within GFF1342 at 685.324 kb on - strand, within GFF1342 at 685.324 kb on - strand, within GFF1342 at 685.324 kb on - strand, within GFF1342 at 685.497 kb on + strand, within GFF1342 at 685.498 kb on - strand, within GFF1342 at 685.498 kb on - strand, within GFF1342 at 685.498 kb on - strand, within GFF1342 at 685.563 kb on + strand, within GFF1342 at 685.569 kb on + strand, within GFF1342 at 685.570 kb on - strand, within GFF1342 at 685.695 kb on + strand, within GFF1342 at 685.695 kb on + strand, within GFF1342 at 685.695 kb on + strand, within GFF1342 at 685.696 kb on - strand, within GFF1342 at 685.696 kb on - strand, within GFF1342 at 685.696 kb on - strand, within GFF1342 at 685.770 kb on + strand at 685.770 kb on + strand at 685.770 kb on + strand at 685.771 kb on - strand at 685.771 kb on - strand at 685.771 kb on - strand at 685.818 kb on + strand at 685.818 kb on + strand at 685.819 kb on - strand at 685.839 kb on + strand at 685.839 kb on + strand at 685.839 kb on + strand at 685.839 kb on + strand at 685.840 kb on - strand at 685.840 kb on - strand at 686.221 kb on + strand, within GFF1343 at 686.222 kb on - strand, within GFF1343 at 686.293 kb on + strand, within GFF1343 at 686.293 kb on + strand, within GFF1343 at 686.294 kb on - strand, within GFF1343 at 686.294 kb on - strand, within GFF1343 at 686.425 kb on + strand, within GFF1343 at 686.425 kb on + strand, within GFF1343 at 686.530 kb on + strand, within GFF1343 at 686.530 kb on + strand, within GFF1343 at 686.530 kb on + strand, within GFF1343 at 686.531 kb on - strand, within GFF1343 at 686.661 kb on - strand, within GFF1343 at 686.714 kb on - strand, within GFF1343
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 682,892 + GFF1340 0.86 +0.6 682,958 + +0.4 682,958 + -1.3 682,959 - -0.6 682,959 - -0.5 683,031 + -1.3 683,032 - -0.2 683,035 - -1.6 683,211 + GFF1341 0.30 -0.3 683,212 - GFF1341 0.30 +0.4 683,212 - GFF1341 0.30 -1.7 683,239 - GFF1341 0.34 -0.2 683,239 - GFF1341 0.34 +0.4 683,346 + GFF1341 0.49 -0.8 683,346 + GFF1341 0.49 -1.7 683,346 + GFF1341 0.49 -0.3 683,346 + GFF1341 0.49 +0.6 683,346 + GFF1341 0.49 -1.2 683,346 + GFF1341 0.49 -0.8 683,346 + GFF1341 0.49 +0.1 683,346 + GFF1341 0.49 +0.4 683,346 + GFF1341 0.49 -0.5 683,346 + GFF1341 0.49 -1.3 683,346 + GFF1341 0.49 +0.0 683,346 + GFF1341 0.49 -0.7 683,347 - GFF1341 0.49 -0.9 683,347 - GFF1341 0.49 +0.0 683,347 - GFF1341 0.49 -2.0 683,347 - GFF1341 0.49 +0.1 683,347 - GFF1341 0.49 -0.3 683,347 - GFF1341 0.49 -1.8 683,347 - GFF1341 0.49 +0.0 683,347 - GFF1341 0.49 -0.1 683,347 - GFF1341 0.49 -0.7 683,520 + GFF1341 0.74 -0.4 683,521 - GFF1341 0.74 +0.6 683,701 - +0.6 683,743 + +0.1 683,744 - +0.1 683,744 - -0.8 683,784 - -1.4 683,862 + -0.5 683,862 + +0.7 683,863 - -0.2 683,863 - +1.4 683,863 - +0.4 684,184 - GFF1342 0.16 +1.1 684,502 - GFF1342 0.31 -0.2 684,502 - GFF1342 0.31 +0.5 684,502 - GFF1342 0.31 +0.2 684,546 + GFF1342 0.33 +0.6 684,885 + GFF1342 0.49 -0.7 684,885 + GFF1342 0.49 -0.3 684,885 + GFF1342 0.49 -0.6 684,886 - GFF1342 0.49 +0.7 684,886 - GFF1342 0.49 +0.4 684,927 + GFF1342 0.51 -0.4 684,927 + GFF1342 0.51 -0.6 684,927 + GFF1342 0.51 +0.2 684,927 + GFF1342 0.51 +0.2 684,927 + GFF1342 0.51 -0.0 685,323 + GFF1342 0.70 +0.4 685,323 + GFF1342 0.70 -0.6 685,323 + GFF1342 0.70 +0.9 685,323 + GFF1342 0.70 +0.9 685,323 + GFF1342 0.70 -0.6 685,324 - GFF1342 0.70 -0.8 685,324 - GFF1342 0.70 -0.1 685,324 - GFF1342 0.70 -0.7 685,497 + GFF1342 0.78 -1.5 685,498 - GFF1342 0.78 -0.8 685,498 - GFF1342 0.78 -0.8 685,498 - GFF1342 0.78 +0.7 685,563 + GFF1342 0.81 +0.7 685,569 + GFF1342 0.82 +1.1 685,570 - GFF1342 0.82 +1.0 685,695 + GFF1342 0.88 +0.8 685,695 + GFF1342 0.88 +0.8 685,695 + GFF1342 0.88 -0.9 685,696 - GFF1342 0.88 -1.8 685,696 - GFF1342 0.88 -0.2 685,696 - GFF1342 0.88 +0.7 685,770 + -0.6 685,770 + -0.4 685,770 + +0.0 685,771 - -0.3 685,771 - -0.1 685,771 - -0.3 685,818 + -0.2 685,818 + -0.8 685,819 - -0.7 685,839 + +0.1 685,839 + +0.7 685,839 + -1.5 685,839 + -0.8 685,840 - +1.4 685,840 - +0.4 686,221 + GFF1343 0.16 -0.5 686,222 - GFF1343 0.16 -1.0 686,293 + GFF1343 0.21 -0.4 686,293 + GFF1343 0.21 -0.6 686,294 - GFF1343 0.21 -0.8 686,294 - GFF1343 0.21 -0.6 686,425 + GFF1343 0.30 +0.7 686,425 + GFF1343 0.30 +0.1 686,530 + GFF1343 0.37 -0.2 686,530 + GFF1343 0.37 +0.0 686,530 + GFF1343 0.37 +0.1 686,531 - GFF1343 0.37 -0.4 686,661 - GFF1343 0.46 +0.6 686,714 - GFF1343 0.49 +0.1
Or see this region's nucleotide sequence