Strain Fitness in Variovorax sp. SCN45 around GFF1293

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1292 and GFF1293 overlap by 4 nucleotidesGFF1293 and GFF1294 overlap by 8 nucleotides GFF1292 - Macrolide-specific efflux protein MacA, at 618,930 to 620,087 GFF1292 GFF1293 - Macrolide export ATP-binding/permease protein MacB, at 620,084 to 622,093 GFF1293 GFF1294 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 622,086 to 623,495 GFF1294 Position (kb) 620 621 622 623Strain fitness (log2 ratio) -2 -1 0 1 2at 619.483 kb on - strand, within GFF1292at 619.483 kb on - strand, within GFF1292at 619.665 kb on + strand, within GFF1292at 619.665 kb on + strand, within GFF1292at 619.665 kb on + strand, within GFF1292at 619.665 kb on + strand, within GFF1292at 619.666 kb on - strand, within GFF1292at 619.699 kb on - strand, within GFF1292at 619.701 kb on + strand, within GFF1292at 619.701 kb on + strand, within GFF1292at 619.701 kb on + strand, within GFF1292at 619.701 kb on + strand, within GFF1292at 619.701 kb on + strand, within GFF1292at 619.702 kb on - strand, within GFF1292at 620.300 kb on + strand, within GFF1293at 620.300 kb on + strand, within GFF1293at 620.300 kb on + strand, within GFF1293at 620.300 kb on + strand, within GFF1293at 620.301 kb on - strand, within GFF1293at 620.301 kb on - strand, within GFF1293at 620.301 kb on - strand, within GFF1293at 620.301 kb on - strand, within GFF1293at 620.301 kb on - strand, within GFF1293at 620.390 kb on + strand, within GFF1293at 620.390 kb on + strand, within GFF1293at 620.434 kb on - strand, within GFF1293at 620.444 kb on + strand, within GFF1293at 620.444 kb on + strand, within GFF1293at 620.445 kb on - strand, within GFF1293at 621.060 kb on - strand, within GFF1293at 621.129 kb on + strand, within GFF1293at 621.129 kb on + strand, within GFF1293at 621.129 kb on + strand, within GFF1293at 621.129 kb on + strand, within GFF1293at 621.129 kb on + strand, within GFF1293at 621.130 kb on - strand, within GFF1293at 621.130 kb on - strand, within GFF1293at 621.130 kb on - strand, within GFF1293at 621.130 kb on - strand, within GFF1293at 621.130 kb on - strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.133 kb on + strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.134 kb on - strand, within GFF1293at 621.176 kb on + strand, within GFF1293at 621.176 kb on + strand, within GFF1293at 621.177 kb on - strand, within GFF1293at 621.177 kb on - strand, within GFF1293at 621.177 kb on - strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.180 kb on + strand, within GFF1293at 621.181 kb on - strand, within GFF1293at 621.181 kb on - strand, within GFF1293at 621.181 kb on - strand, within GFF1293at 621.512 kb on + strand, within GFF1293at 621.921 kb on - strandat 621.921 kb on - strandat 622.195 kb on - strandat 622.357 kb on + strand, within GFF1294at 622.357 kb on + strand, within GFF1294at 622.358 kb on - strand, within GFF1294at 622.358 kb on - strand, within GFF1294at 622.358 kb on - strand, within GFF1294at 622.447 kb on - strand, within GFF1294at 622.447 kb on - strand, within GFF1294at 622.506 kb on + strand, within GFF1294at 622.506 kb on + strand, within GFF1294at 622.507 kb on - strand, within GFF1294at 622.629 kb on + strand, within GFF1294at 622.630 kb on - strand, within GFF1294at 622.644 kb on + strand, within GFF1294at 622.722 kb on + strand, within GFF1294at 622.723 kb on - strand, within GFF1294at 623.026 kb on - strand, within GFF1294

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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619,483 - GFF1292 0.48 -0.4
619,483 - GFF1292 0.48 -0.7
619,665 + GFF1292 0.63 -0.7
619,665 + GFF1292 0.63 +0.8
619,665 + GFF1292 0.63 -1.0
619,665 + GFF1292 0.63 -0.2
619,666 - GFF1292 0.64 -0.4
619,699 - GFF1292 0.66 -0.4
619,701 + GFF1292 0.67 -0.5
619,701 + GFF1292 0.67 +0.1
619,701 + GFF1292 0.67 +0.4
619,701 + GFF1292 0.67 +0.6
619,701 + GFF1292 0.67 -0.2
619,702 - GFF1292 0.67 -1.2
620,300 + GFF1293 0.11 -0.7
620,300 + GFF1293 0.11 +0.8
620,300 + GFF1293 0.11 +1.0
620,300 + GFF1293 0.11 +0.0
620,301 - GFF1293 0.11 -0.8
620,301 - GFF1293 0.11 -0.2
620,301 - GFF1293 0.11 -0.2
620,301 - GFF1293 0.11 +1.4
620,301 - GFF1293 0.11 +0.4
620,390 + GFF1293 0.15 +0.0
620,390 + GFF1293 0.15 +0.5
620,434 - GFF1293 0.17 +0.1
620,444 + GFF1293 0.18 -0.2
620,444 + GFF1293 0.18 +0.2
620,445 - GFF1293 0.18 -2.2
621,060 - GFF1293 0.49 +1.0
621,129 + GFF1293 0.52 -0.2
621,129 + GFF1293 0.52 +0.6
621,129 + GFF1293 0.52 +0.6
621,129 + GFF1293 0.52 -0.8
621,129 + GFF1293 0.52 +0.0
621,130 - GFF1293 0.52 -2.2
621,130 - GFF1293 0.52 +0.1
621,130 - GFF1293 0.52 +0.2
621,130 - GFF1293 0.52 +0.4
621,130 - GFF1293 0.52 +0.3
621,133 + GFF1293 0.52 +0.2
621,133 + GFF1293 0.52 +1.8
621,133 + GFF1293 0.52 +0.4
621,133 + GFF1293 0.52 +0.1
621,133 + GFF1293 0.52 -0.3
621,133 + GFF1293 0.52 +0.1
621,133 + GFF1293 0.52 +1.0
621,133 + GFF1293 0.52 -0.6
621,133 + GFF1293 0.52 +0.4
621,133 + GFF1293 0.52 -0.6
621,133 + GFF1293 0.52 -0.6
621,133 + GFF1293 0.52 +0.2
621,134 - GFF1293 0.52 -0.5
621,134 - GFF1293 0.52 +0.4
621,134 - GFF1293 0.52 -0.5
621,134 - GFF1293 0.52 +0.2
621,134 - GFF1293 0.52 +0.5
621,134 - GFF1293 0.52 +0.2
621,134 - GFF1293 0.52 -0.6
621,134 - GFF1293 0.52 -0.5
621,176 + GFF1293 0.54 +0.3
621,176 + GFF1293 0.54 -0.4
621,177 - GFF1293 0.54 +0.5
621,177 - GFF1293 0.54 +0.8
621,177 - GFF1293 0.54 +0.2
621,180 + GFF1293 0.55 -0.4
621,180 + GFF1293 0.55 -0.5
621,180 + GFF1293 0.55 -0.3
621,180 + GFF1293 0.55 +0.6
621,180 + GFF1293 0.55 -0.4
621,180 + GFF1293 0.55 +0.1
621,180 + GFF1293 0.55 -1.9
621,181 - GFF1293 0.55 -0.8
621,181 - GFF1293 0.55 +1.3
621,181 - GFF1293 0.55 -1.7
621,512 + GFF1293 0.71 -0.6
621,921 - +1.1
621,921 - -1.6
622,195 - -1.7
622,357 + GFF1294 0.19 +0.0
622,357 + GFF1294 0.19 -0.2
622,358 - GFF1294 0.19 +0.0
622,358 - GFF1294 0.19 +0.5
622,358 - GFF1294 0.19 -0.7
622,447 - GFF1294 0.26 -0.4
622,447 - GFF1294 0.26 -1.2
622,506 + GFF1294 0.30 -0.9
622,506 + GFF1294 0.30 +0.7
622,507 - GFF1294 0.30 +0.7
622,629 + GFF1294 0.39 -0.9
622,630 - GFF1294 0.39 +0.2
622,644 + GFF1294 0.40 +0.1
622,722 + GFF1294 0.45 -0.2
622,723 - GFF1294 0.45 -0.5
623,026 - GFF1294 0.67 -0.3

Or see this region's nucleotide sequence