Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1237 and GFF1238 are separated by 98 nucleotides GFF1238 and GFF1239 overlap by 1 nucleotides GFF1239 and GFF1240 are separated by 78 nucleotides
GFF1237 - Methionine sulfoxide reductase cytochrome b subunit, at 555,846 to 556,502
GFF1237
GFF1238 - Two-component transcriptional response regulator, OmpR family, at 556,601 to 557,326
GFF1238
GFF1239 - Two-component system sensor histidine kinase, at 557,326 to 558,822
GFF1239
GFF1240 - Enoyl-CoA hydratase (EC 4.2.1.17), at 558,901 to 559,824
GFF1240
Position (kb) 557
558
559 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 556.351 kb on + strand, within GFF1237 at 556.555 kb on + strand at 556.555 kb on + strand at 556.556 kb on - strand at 556.556 kb on - strand at 556.556 kb on - strand at 556.685 kb on + strand, within GFF1238 at 556.685 kb on + strand, within GFF1238 at 556.826 kb on + strand, within GFF1238 at 556.826 kb on + strand, within GFF1238 at 556.826 kb on + strand, within GFF1238 at 556.827 kb on - strand, within GFF1238 at 556.827 kb on - strand, within GFF1238 at 556.827 kb on - strand, within GFF1238 at 556.904 kb on + strand, within GFF1238 at 556.904 kb on + strand, within GFF1238 at 556.904 kb on + strand, within GFF1238 at 556.905 kb on - strand, within GFF1238 at 556.905 kb on - strand, within GFF1238 at 556.905 kb on - strand, within GFF1238 at 557.105 kb on + strand, within GFF1238 at 557.105 kb on + strand, within GFF1238 at 557.106 kb on - strand, within GFF1238 at 557.172 kb on - strand, within GFF1238 at 557.186 kb on + strand, within GFF1238 at 557.285 kb on + strand at 557.285 kb on + strand at 557.286 kb on - strand at 557.286 kb on - strand at 557.581 kb on + strand, within GFF1239 at 557.581 kb on + strand, within GFF1239 at 557.581 kb on + strand, within GFF1239 at 557.582 kb on - strand, within GFF1239 at 557.582 kb on - strand, within GFF1239 at 557.582 kb on - strand, within GFF1239 at 557.582 kb on - strand, within GFF1239 at 557.582 kb on - strand, within GFF1239 at 557.782 kb on + strand, within GFF1239 at 557.783 kb on - strand, within GFF1239 at 557.783 kb on - strand, within GFF1239 at 557.783 kb on - strand, within GFF1239 at 557.783 kb on - strand, within GFF1239 at 557.783 kb on - strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.788 kb on + strand, within GFF1239 at 557.789 kb on - strand, within GFF1239 at 557.789 kb on - strand, within GFF1239 at 558.190 kb on + strand, within GFF1239 at 558.247 kb on + strand, within GFF1239 at 558.326 kb on - strand, within GFF1239 at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.855 kb on + strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 558.856 kb on - strand at 559.020 kb on - strand, within GFF1240 at 559.020 kb on - strand, within GFF1240 at 559.120 kb on + strand, within GFF1240 at 559.120 kb on + strand, within GFF1240 at 559.121 kb on - strand, within GFF1240 at 559.121 kb on - strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.201 kb on + strand, within GFF1240 at 559.202 kb on - strand, within GFF1240 at 559.202 kb on - strand, within GFF1240 at 559.357 kb on + strand, within GFF1240 at 559.357 kb on + strand, within GFF1240 at 559.357 kb on + strand, within GFF1240 at 559.358 kb on - strand, within GFF1240 at 559.358 kb on - strand, within GFF1240 at 559.358 kb on - strand, within GFF1240 at 559.406 kb on - strand, within GFF1240
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 556,351 + GFF1237 0.77 -0.4 556,555 + -0.4 556,555 + +0.1 556,556 - +0.1 556,556 - +0.8 556,556 - -0.9 556,685 + GFF1238 0.12 -1.6 556,685 + GFF1238 0.12 +1.0 556,826 + GFF1238 0.31 +0.4 556,826 + GFF1238 0.31 +0.2 556,826 + GFF1238 0.31 +0.4 556,827 - GFF1238 0.31 +0.2 556,827 - GFF1238 0.31 -0.4 556,827 - GFF1238 0.31 +0.2 556,904 + GFF1238 0.42 +0.4 556,904 + GFF1238 0.42 -0.4 556,904 + GFF1238 0.42 -0.5 556,905 - GFF1238 0.42 +0.6 556,905 - GFF1238 0.42 -2.4 556,905 - GFF1238 0.42 -0.6 557,105 + GFF1238 0.69 -0.2 557,105 + GFF1238 0.69 -1.7 557,106 - GFF1238 0.70 -0.9 557,172 - GFF1238 0.79 -0.2 557,186 + GFF1238 0.81 +1.7 557,285 + +0.4 557,285 + +0.4 557,286 - -1.0 557,286 - +0.6 557,581 + GFF1239 0.17 +0.3 557,581 + GFF1239 0.17 -0.9 557,581 + GFF1239 0.17 -0.7 557,582 - GFF1239 0.17 -0.2 557,582 - GFF1239 0.17 -1.4 557,582 - GFF1239 0.17 -3.5 557,582 - GFF1239 0.17 -0.2 557,582 - GFF1239 0.17 -1.9 557,782 + GFF1239 0.30 +0.9 557,783 - GFF1239 0.31 +0.4 557,783 - GFF1239 0.31 -2.0 557,783 - GFF1239 0.31 +0.8 557,783 - GFF1239 0.31 -0.6 557,783 - GFF1239 0.31 -0.4 557,788 + GFF1239 0.31 -1.9 557,788 + GFF1239 0.31 -0.9 557,788 + GFF1239 0.31 -0.4 557,788 + GFF1239 0.31 +0.4 557,788 + GFF1239 0.31 -2.2 557,788 + GFF1239 0.31 +0.0 557,788 + GFF1239 0.31 -2.0 557,788 + GFF1239 0.31 -0.2 557,788 + GFF1239 0.31 -1.2 557,789 - GFF1239 0.31 -0.2 557,789 - GFF1239 0.31 +0.4 558,190 + GFF1239 0.58 -0.2 558,247 + GFF1239 0.62 +1.0 558,326 - GFF1239 0.67 -0.2 558,855 + -0.2 558,855 + -1.9 558,855 + -0.8 558,855 + +1.4 558,855 + +1.2 558,855 + -1.0 558,855 + +0.7 558,855 + -0.9 558,856 - -0.2 558,856 - +1.2 558,856 - -2.4 558,856 - -1.2 558,856 - +0.3 558,856 - -0.0 558,856 - -1.4 558,856 - -0.9 558,856 - -0.2 559,020 - GFF1240 0.13 -0.8 559,020 - GFF1240 0.13 +1.8 559,120 + GFF1240 0.24 +0.3 559,120 + GFF1240 0.24 -1.4 559,121 - GFF1240 0.24 -0.8 559,121 - GFF1240 0.24 -0.4 559,201 + GFF1240 0.32 +0.0 559,201 + GFF1240 0.32 -0.6 559,201 + GFF1240 0.32 +1.0 559,201 + GFF1240 0.32 +0.0 559,201 + GFF1240 0.32 -0.8 559,201 + GFF1240 0.32 -0.6 559,201 + GFF1240 0.32 -1.0 559,201 + GFF1240 0.32 +1.4 559,202 - GFF1240 0.33 -0.9 559,202 - GFF1240 0.33 -0.1 559,357 + GFF1240 0.49 +0.3 559,357 + GFF1240 0.49 +0.7 559,357 + GFF1240 0.49 -2.2 559,358 - GFF1240 0.49 -0.3 559,358 - GFF1240 0.49 +0.6 559,358 - GFF1240 0.49 +0.2 559,406 - GFF1240 0.55 -0.9
Or see this region's nucleotide sequence