Strain Fitness in Variovorax sp. SCN45 around GFF1237

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1235 and GFF1236 are separated by 104 nucleotidesGFF1236 and GFF1237 are separated by 7 nucleotidesGFF1237 and GFF1238 are separated by 98 nucleotidesGFF1238 and GFF1239 overlap by 1 nucleotides GFF1235 - Methionine sulfoxide reductase-associated methionine-rich protein, at 554,676 to 554,942 GFF1235 GFF1236 - Methionine sulfoxide reductase molybdopterin-binding subunit, at 555,047 to 555,838 GFF1236 GFF1237 - Methionine sulfoxide reductase cytochrome b subunit, at 555,846 to 556,502 GFF1237 GFF1238 - Two-component transcriptional response regulator, OmpR family, at 556,601 to 557,326 GFF1238 GFF1239 - Two-component system sensor histidine kinase, at 557,326 to 558,822 GFF1239 Position (kb) 555 556 557Strain fitness (log2 ratio) -2 -1 0 1at 555.069 kb on + strandat 555.069 kb on + strandat 555.088 kb on - strandat 555.099 kb on + strandat 555.099 kb on + strandat 555.100 kb on - strandat 555.144 kb on + strand, within GFF1236at 555.145 kb on - strand, within GFF1236at 555.145 kb on - strand, within GFF1236at 555.186 kb on + strand, within GFF1236at 555.186 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.270 kb on + strand, within GFF1236at 555.271 kb on - strand, within GFF1236at 555.271 kb on - strand, within GFF1236at 555.271 kb on - strand, within GFF1236at 555.273 kb on + strand, within GFF1236at 555.273 kb on + strand, within GFF1236at 555.273 kb on + strand, within GFF1236at 555.274 kb on - strand, within GFF1236at 555.274 kb on - strand, within GFF1236at 555.300 kb on + strand, within GFF1236at 555.300 kb on + strand, within GFF1236at 555.300 kb on + strand, within GFF1236at 555.300 kb on + strand, within GFF1236at 555.301 kb on - strand, within GFF1236at 555.301 kb on - strand, within GFF1236at 555.301 kb on - strand, within GFF1236at 555.301 kb on - strand, within GFF1236at 555.489 kb on + strand, within GFF1236at 555.489 kb on + strand, within GFF1236at 555.489 kb on + strand, within GFF1236at 555.490 kb on - strand, within GFF1236at 555.528 kb on + strand, within GFF1236at 555.528 kb on + strand, within GFF1236at 555.528 kb on + strand, within GFF1236at 555.529 kb on - strand, within GFF1236at 555.529 kb on - strand, within GFF1236at 555.531 kb on + strand, within GFF1236at 555.532 kb on - strand, within GFF1236at 555.573 kb on + strand, within GFF1236at 555.574 kb on - strand, within GFF1236at 556.079 kb on - strand, within GFF1237at 556.079 kb on - strand, within GFF1237at 556.102 kb on + strand, within GFF1237at 556.103 kb on - strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.228 kb on + strand, within GFF1237at 556.229 kb on - strand, within GFF1237at 556.351 kb on + strand, within GFF1237at 556.555 kb on + strandat 556.555 kb on + strandat 556.556 kb on - strandat 556.556 kb on - strandat 556.556 kb on - strandat 556.685 kb on + strand, within GFF1238at 556.685 kb on + strand, within GFF1238at 556.826 kb on + strand, within GFF1238at 556.826 kb on + strand, within GFF1238at 556.826 kb on + strand, within GFF1238at 556.827 kb on - strand, within GFF1238at 556.827 kb on - strand, within GFF1238at 556.827 kb on - strand, within GFF1238at 556.904 kb on + strand, within GFF1238at 556.904 kb on + strand, within GFF1238at 556.904 kb on + strand, within GFF1238at 556.905 kb on - strand, within GFF1238at 556.905 kb on - strand, within GFF1238at 556.905 kb on - strand, within GFF1238at 557.105 kb on + strand, within GFF1238at 557.105 kb on + strand, within GFF1238at 557.106 kb on - strand, within GFF1238at 557.172 kb on - strand, within GFF1238at 557.186 kb on + strand, within GFF1238at 557.285 kb on + strandat 557.285 kb on + strandat 557.286 kb on - strandat 557.286 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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555,069 + +1.2
555,069 + -0.9
555,088 - -0.7
555,099 + -0.6
555,099 + -0.9
555,100 - +1.1
555,144 + GFF1236 0.12 +0.3
555,145 - GFF1236 0.12 -0.6
555,145 - GFF1236 0.12 -0.4
555,186 + GFF1236 0.18 +0.4
555,186 + GFF1236 0.18 -0.6
555,270 + GFF1236 0.28 -0.7
555,270 + GFF1236 0.28 -0.8
555,270 + GFF1236 0.28 -0.6
555,270 + GFF1236 0.28 -0.6
555,270 + GFF1236 0.28 +0.6
555,270 + GFF1236 0.28 -1.2
555,271 - GFF1236 0.28 +0.9
555,271 - GFF1236 0.28 +0.9
555,271 - GFF1236 0.28 -0.8
555,273 + GFF1236 0.29 -0.0
555,273 + GFF1236 0.29 +0.2
555,273 + GFF1236 0.29 +0.1
555,274 - GFF1236 0.29 -0.7
555,274 - GFF1236 0.29 -0.6
555,300 + GFF1236 0.32 +0.7
555,300 + GFF1236 0.32 +1.3
555,300 + GFF1236 0.32 +0.6
555,300 + GFF1236 0.32 +0.6
555,301 - GFF1236 0.32 -0.0
555,301 - GFF1236 0.32 +0.4
555,301 - GFF1236 0.32 +0.8
555,301 - GFF1236 0.32 +0.4
555,489 + GFF1236 0.56 -0.5
555,489 + GFF1236 0.56 -0.2
555,489 + GFF1236 0.56 -0.6
555,490 - GFF1236 0.56 -1.7
555,528 + GFF1236 0.61 -1.1
555,528 + GFF1236 0.61 +0.0
555,528 + GFF1236 0.61 +0.2
555,529 - GFF1236 0.61 -1.8
555,529 - GFF1236 0.61 +0.1
555,531 + GFF1236 0.61 -0.4
555,532 - GFF1236 0.61 +0.4
555,573 + GFF1236 0.66 -0.5
555,574 - GFF1236 0.67 -0.9
556,079 - GFF1237 0.35 -1.2
556,079 - GFF1237 0.35 -0.7
556,102 + GFF1237 0.39 -1.1
556,103 - GFF1237 0.39 +0.0
556,228 + GFF1237 0.58 -0.4
556,228 + GFF1237 0.58 +0.0
556,228 + GFF1237 0.58 -1.6
556,228 + GFF1237 0.58 +0.0
556,228 + GFF1237 0.58 +0.3
556,228 + GFF1237 0.58 -1.6
556,229 - GFF1237 0.58 -2.2
556,351 + GFF1237 0.77 -0.4
556,555 + -0.4
556,555 + +0.1
556,556 - +0.1
556,556 - +0.8
556,556 - -0.9
556,685 + GFF1238 0.12 -1.6
556,685 + GFF1238 0.12 +1.0
556,826 + GFF1238 0.31 +0.4
556,826 + GFF1238 0.31 +0.2
556,826 + GFF1238 0.31 +0.4
556,827 - GFF1238 0.31 +0.2
556,827 - GFF1238 0.31 -0.4
556,827 - GFF1238 0.31 +0.2
556,904 + GFF1238 0.42 +0.4
556,904 + GFF1238 0.42 -0.4
556,904 + GFF1238 0.42 -0.5
556,905 - GFF1238 0.42 +0.6
556,905 - GFF1238 0.42 -2.4
556,905 - GFF1238 0.42 -0.6
557,105 + GFF1238 0.69 -0.2
557,105 + GFF1238 0.69 -1.7
557,106 - GFF1238 0.70 -0.9
557,172 - GFF1238 0.79 -0.2
557,186 + GFF1238 0.81 +1.7
557,285 + +0.4
557,285 + +0.4
557,286 - -1.0
557,286 - +0.6

Or see this region's nucleotide sequence