Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1144 and GFF1145 are separated by 48 nucleotides GFF1145 and GFF1146 are separated by 1 nucleotides
GFF1144 - hypothetical protein, at 459,799 to 461,700
GFF1144
GFF1145 - hypothetical protein, at 461,749 to 462,117
GFF1145
GFF1146 - Pyrophosphate-energized proton pump (EC 3.6.1.1), at 462,119 to 464,635
GFF1146
Position (kb)
461
462
463 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 461.063 kb on + strand, within GFF1144 at 461.063 kb on + strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.064 kb on - strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.255 kb on + strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.256 kb on - strand, within GFF1144 at 461.276 kb on + strand, within GFF1144 at 461.276 kb on + strand, within GFF1144 at 461.276 kb on + strand, within GFF1144 at 461.276 kb on + strand, within GFF1144 at 461.277 kb on - strand, within GFF1144 at 461.277 kb on - strand, within GFF1144 at 461.277 kb on - strand, within GFF1144 at 461.277 kb on - strand, within GFF1144 at 461.277 kb on - strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.483 kb on + strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.484 kb on - strand, within GFF1144 at 461.564 kb on + strand at 461.564 kb on + strand at 461.564 kb on + strand at 461.565 kb on - strand at 461.565 kb on - strand at 461.576 kb on + strand at 461.576 kb on + strand at 461.576 kb on + strand at 461.576 kb on + strand at 461.576 kb on + strand at 461.577 kb on - strand at 461.577 kb on - strand at 461.577 kb on - strand at 461.577 kb on - strand at 461.577 kb on - strand at 461.577 kb on - strand at 461.693 kb on + strand at 461.694 kb on - strand at 461.694 kb on - strand at 461.777 kb on + strand at 461.777 kb on + strand at 461.778 kb on - strand at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.038 kb on + strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.039 kb on - strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.041 kb on + strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.042 kb on - strand, within GFF1145 at 462.128 kb on + strand at 462.128 kb on + strand at 462.129 kb on - strand at 462.129 kb on - strand at 462.129 kb on - strand at 462.129 kb on - strand at 462.408 kb on + strand, within GFF1146 at 462.408 kb on + strand, within GFF1146 at 462.408 kb on + strand, within GFF1146 at 462.409 kb on - strand, within GFF1146 at 462.669 kb on + strand, within GFF1146 at 462.670 kb on - strand, within GFF1146 at 462.670 kb on - strand, within GFF1146 at 462.670 kb on - strand, within GFF1146 at 462.670 kb on - strand, within GFF1146 at 462.670 kb on - strand, within GFF1146 at 462.750 kb on + strand, within GFF1146 at 462.750 kb on + strand, within GFF1146 at 462.750 kb on + strand, within GFF1146 at 462.750 kb on + strand, within GFF1146 at 462.751 kb on - strand, within GFF1146 at 462.751 kb on - strand, within GFF1146 at 462.751 kb on - strand, within GFF1146 at 462.751 kb on - strand, within GFF1146 at 462.751 kb on - strand, within GFF1146 at 462.957 kb on + strand, within GFF1146 at 462.957 kb on + strand, within GFF1146 at 462.958 kb on - strand, within GFF1146 at 463.078 kb on - strand, within GFF1146
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 461,063 + GFF1144 0.66 +0.1 461,063 + GFF1144 0.66 -0.9 461,064 - GFF1144 0.67 +1.1 461,064 - GFF1144 0.67 -0.4 461,064 - GFF1144 0.67 +0.0 461,064 - GFF1144 0.67 +0.5 461,064 - GFF1144 0.67 -0.3 461,064 - GFF1144 0.67 +0.3 461,255 + GFF1144 0.77 -0.2 461,255 + GFF1144 0.77 -0.2 461,255 + GFF1144 0.77 +0.3 461,255 + GFF1144 0.77 -0.1 461,255 + GFF1144 0.77 -0.0 461,255 + GFF1144 0.77 +0.0 461,255 + GFF1144 0.77 -0.6 461,255 + GFF1144 0.77 +1.2 461,255 + GFF1144 0.77 +0.1 461,256 - GFF1144 0.77 -2.9 461,256 - GFF1144 0.77 -1.1 461,256 - GFF1144 0.77 -0.1 461,256 - GFF1144 0.77 +0.9 461,256 - GFF1144 0.77 +0.4 461,256 - GFF1144 0.77 -0.5 461,256 - GFF1144 0.77 +0.3 461,256 - GFF1144 0.77 -0.1 461,256 - GFF1144 0.77 -0.6 461,256 - GFF1144 0.77 -1.2 461,256 - GFF1144 0.77 -0.3 461,276 + GFF1144 0.78 -0.9 461,276 + GFF1144 0.78 -0.1 461,276 + GFF1144 0.78 -0.9 461,276 + GFF1144 0.78 +0.3 461,277 - GFF1144 0.78 +0.0 461,277 - GFF1144 0.78 -1.1 461,277 - GFF1144 0.78 -1.2 461,277 - GFF1144 0.78 -0.2 461,277 - GFF1144 0.78 -0.4 461,483 + GFF1144 0.89 +1.1 461,483 + GFF1144 0.89 +0.0 461,483 + GFF1144 0.89 +0.6 461,483 + GFF1144 0.89 +0.0 461,483 + GFF1144 0.89 -0.9 461,483 + GFF1144 0.89 -0.8 461,483 + GFF1144 0.89 -0.2 461,483 + GFF1144 0.89 -0.6 461,483 + GFF1144 0.89 -0.3 461,483 + GFF1144 0.89 +0.4 461,483 + GFF1144 0.89 +0.3 461,484 - GFF1144 0.89 +0.7 461,484 - GFF1144 0.89 +0.1 461,484 - GFF1144 0.89 -1.1 461,484 - GFF1144 0.89 +0.4 461,484 - GFF1144 0.89 -0.5 461,484 - GFF1144 0.89 -2.5 461,484 - GFF1144 0.89 -0.7 461,484 - GFF1144 0.89 +0.7 461,484 - GFF1144 0.89 +0.5 461,484 - GFF1144 0.89 -0.5 461,484 - GFF1144 0.89 +0.5 461,484 - GFF1144 0.89 -1.6 461,484 - GFF1144 0.89 -0.7 461,484 - GFF1144 0.89 -0.0 461,484 - GFF1144 0.89 -1.7 461,484 - GFF1144 0.89 -1.1 461,564 + -0.5 461,564 + -1.3 461,564 + -2.4 461,565 - -0.7 461,565 - +0.6 461,576 + +1.0 461,576 + -2.4 461,576 + -0.9 461,576 + -0.6 461,576 + +0.2 461,577 - +0.6 461,577 - -0.4 461,577 - -0.2 461,577 - +0.7 461,577 - -1.4 461,577 - +0.4 461,693 + -0.9 461,694 - -0.1 461,694 - +0.9 461,777 + -1.1 461,777 + +0.1 461,778 - +0.1 462,038 + GFF1145 0.78 -0.3 462,038 + GFF1145 0.78 -0.4 462,038 + GFF1145 0.78 -0.6 462,038 + GFF1145 0.78 +1.4 462,038 + GFF1145 0.78 +0.3 462,038 + GFF1145 0.78 -2.3 462,038 + GFF1145 0.78 -1.9 462,038 + GFF1145 0.78 -0.1 462,038 + GFF1145 0.78 -1.4 462,039 - GFF1145 0.79 +0.0 462,039 - GFF1145 0.79 -0.8 462,039 - GFF1145 0.79 -1.5 462,039 - GFF1145 0.79 -0.6 462,039 - GFF1145 0.79 -0.9 462,039 - GFF1145 0.79 -0.7 462,041 + GFF1145 0.79 -0.8 462,041 + GFF1145 0.79 +1.4 462,041 + GFF1145 0.79 +0.1 462,041 + GFF1145 0.79 +0.9 462,041 + GFF1145 0.79 +1.4 462,041 + GFF1145 0.79 -0.7 462,041 + GFF1145 0.79 -0.5 462,041 + GFF1145 0.79 +0.3 462,042 - GFF1145 0.79 -0.6 462,042 - GFF1145 0.79 -0.2 462,042 - GFF1145 0.79 -0.9 462,042 - GFF1145 0.79 -1.9 462,042 - GFF1145 0.79 +0.1 462,042 - GFF1145 0.79 -0.5 462,128 + -0.6 462,128 + +0.3 462,129 - +1.0 462,129 - -0.1 462,129 - -1.1 462,129 - -2.0 462,408 + GFF1146 0.11 +1.0 462,408 + GFF1146 0.11 +0.5 462,408 + GFF1146 0.11 +1.0 462,409 - GFF1146 0.12 -0.5 462,669 + GFF1146 0.22 -1.0 462,670 - GFF1146 0.22 +0.4 462,670 - GFF1146 0.22 +0.1 462,670 - GFF1146 0.22 +0.6 462,670 - GFF1146 0.22 +0.3 462,670 - GFF1146 0.22 +0.8 462,750 + GFF1146 0.25 +0.0 462,750 + GFF1146 0.25 +0.8 462,750 + GFF1146 0.25 -0.1 462,750 + GFF1146 0.25 +0.7 462,751 - GFF1146 0.25 +0.0 462,751 - GFF1146 0.25 -0.8 462,751 - GFF1146 0.25 +1.1 462,751 - GFF1146 0.25 +0.6 462,751 - GFF1146 0.25 -0.1 462,957 + GFF1146 0.33 -0.2 462,957 + GFF1146 0.33 +2.4 462,958 - GFF1146 0.33 +1.0 463,078 - GFF1146 0.38 +0.3
Or see this region's nucleotide sequence