Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt relA and mazE are separated by 77 nucleotides mazE and mazF overlap by 1 nucleotides mazF and mazG are separated by 70 nucleotides mazG and pyrG are separated by 227 nucleotides
BNILDI_10630: relA - GTP diphosphokinase, at 2,147,943 to 2,150,177
relA
BNILDI_10635: mazE - type II toxin-antitoxin system antitoxin MazE, at 2,150,255 to 2,150,503
mazE
BNILDI_10640: mazF - endoribonuclease MazF, at 2,150,503 to 2,150,838
mazF
BNILDI_10645: mazG - nucleoside triphosphate pyrophosphohydrolase, at 2,150,909 to 2,151,700
mazG
BNILDI_10650: pyrG - CTP synthase (glutamine hydrolyzing), at 2,151,928 to 2,153,565
pyrG
Position (kb)
2150
2151
2152 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2149.924 kb on - strand, within relA at 2149.970 kb on + strand at 2149.981 kb on + strand at 2149.981 kb on + strand at 2149.982 kb on - strand at 2149.989 kb on - strand at 2149.989 kb on - strand at 2150.271 kb on + strand at 2150.383 kb on + strand, within mazE at 2150.384 kb on - strand, within mazE at 2150.413 kb on + strand, within mazE at 2150.414 kb on - strand, within mazE at 2150.507 kb on + strand at 2150.617 kb on + strand, within mazF at 2150.618 kb on - strand, within mazF at 2150.634 kb on + strand, within mazF at 2150.634 kb on + strand, within mazF at 2150.723 kb on + strand, within mazF at 2150.742 kb on + strand, within mazF at 2150.798 kb on + strand, within mazF at 2150.836 kb on + strand at 2150.842 kb on + strand at 2150.936 kb on - strand at 2150.938 kb on + strand at 2151.011 kb on + strand, within mazG at 2151.011 kb on + strand, within mazG at 2151.011 kb on + strand, within mazG at 2151.151 kb on + strand, within mazG at 2151.333 kb on + strand, within mazG at 2151.440 kb on + strand, within mazG at 2151.441 kb on - strand, within mazG at 2151.441 kb on - strand, within mazG at 2151.441 kb on - strand, within mazG at 2151.605 kb on - strand, within mazG
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 2,149,924 - relA BNILDI_10630 0.89 -1.0 2,149,970 + +0.3 2,149,981 + +0.5 2,149,981 + +0.3 2,149,982 - +0.3 2,149,989 - +2.4 2,149,989 - -1.8 2,150,271 + -1.0 2,150,383 + mazE BNILDI_10635 0.51 -2.1 2,150,384 - mazE BNILDI_10635 0.52 -0.1 2,150,413 + mazE BNILDI_10635 0.63 +1.8 2,150,414 - mazE BNILDI_10635 0.64 +2.2 2,150,507 + -0.2 2,150,617 + mazF BNILDI_10640 0.34 -1.2 2,150,618 - mazF BNILDI_10640 0.34 +0.4 2,150,634 + mazF BNILDI_10640 0.39 -0.8 2,150,634 + mazF BNILDI_10640 0.39 +1.1 2,150,723 + mazF BNILDI_10640 0.65 +0.5 2,150,742 + mazF BNILDI_10640 0.71 +0.5 2,150,798 + mazF BNILDI_10640 0.88 -0.9 2,150,836 + -0.2 2,150,842 + +0.3 2,150,936 - -1.0 2,150,938 + -0.5 2,151,011 + mazG BNILDI_10645 0.13 +1.5 2,151,011 + mazG BNILDI_10645 0.13 +0.8 2,151,011 + mazG BNILDI_10645 0.13 +1.5 2,151,151 + mazG BNILDI_10645 0.31 -1.4 2,151,333 + mazG BNILDI_10645 0.54 -0.0 2,151,440 + mazG BNILDI_10645 0.67 +0.2 2,151,441 - mazG BNILDI_10645 0.67 +0.1 2,151,441 - mazG BNILDI_10645 0.67 +0.0 2,151,441 - mazG BNILDI_10645 0.67 +0.8 2,151,605 - mazG BNILDI_10645 0.88 +2.1
Or see this region's nucleotide sequence