Strain Fitness in Escherichia coli ECRC62 around BNILDI_10385

Experiment: JK36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlgt and thyA are separated by 6 nucleotidesthyA and ppdA are separated by 184 nucleotidesppdA and BNILDI_10390 overlap by 10 nucleotidesBNILDI_10390 and ygdB overlap by 4 nucleotidesygdB and BNILDI_10400 overlap by 16 nucleotides BNILDI_10375: lgt - prolipoprotein diacylglyceryl transferase, at 2,089,882 to 2,090,757 lgt BNILDI_10380: thyA - thymidylate synthase, at 2,090,764 to 2,091,558 thyA BNILDI_10385: ppdA - prepilin peptidase-dependent protein, at 2,091,743 to 2,092,213 ppdA BNILDI_10390: BNILDI_10390 - Prepilin peptidase dependent protein B, at 2,092,204 to 2,092,767 _10390 BNILDI_10395: ygdB - DUF2509 domain-containing protein, at 2,092,764 to 2,093,171 ygdB BNILDI_10400: BNILDI_10400 - DUF2509 domain-containing protein, at 2,093,156 to 2,093,479 _10400 Position (kb) 2091 2092 2093Strain fitness (log2 ratio) -2 -1 0 1 2at 2091.556 kb on + strandat 2091.556 kb on + strandat 2091.557 kb on - strandat 2091.557 kb on - strandat 2091.557 kb on - strandat 2091.667 kb on + strandat 2091.696 kb on + strandat 2091.763 kb on + strandat 2091.763 kb on + strandat 2091.763 kb on + strandat 2091.764 kb on - strandat 2091.825 kb on - strand, within ppdAat 2091.887 kb on + strand, within ppdAat 2091.896 kb on + strand, within ppdAat 2091.896 kb on + strand, within ppdAat 2091.897 kb on - strand, within ppdAat 2091.901 kb on - strand, within ppdAat 2091.937 kb on + strand, within ppdAat 2091.938 kb on - strand, within ppdAat 2091.997 kb on + strand, within ppdAat 2092.252 kb on - strandat 2092.268 kb on + strand, within BNILDI_10390at 2092.269 kb on - strand, within BNILDI_10390at 2092.458 kb on + strand, within BNILDI_10390at 2092.650 kb on - strand, within BNILDI_10390at 2092.692 kb on + strand, within BNILDI_10390at 2092.707 kb on + strand, within BNILDI_10390at 2092.741 kb on + strandat 2092.742 kb on - strandat 2092.743 kb on + strandat 2092.984 kb on - strand, within ygdBat 2093.018 kb on - strand, within ygdBat 2093.155 kb on + strandat 2093.155 kb on + strandat 2093.155 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK36
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2,091,556 + +1.8
2,091,556 + +0.6
2,091,557 - -0.3
2,091,557 - -0.9
2,091,557 - +1.5
2,091,667 + +0.3
2,091,696 + +0.1
2,091,763 + +0.1
2,091,763 + -0.3
2,091,763 + -0.5
2,091,764 - -0.3
2,091,825 - ppdA BNILDI_10385 0.17 -1.5
2,091,887 + ppdA BNILDI_10385 0.31 +0.3
2,091,896 + ppdA BNILDI_10385 0.32 +0.0
2,091,896 + ppdA BNILDI_10385 0.32 -1.4
2,091,897 - ppdA BNILDI_10385 0.33 -1.1
2,091,901 - ppdA BNILDI_10385 0.34 -2.1
2,091,937 + ppdA BNILDI_10385 0.41 +0.3
2,091,938 - ppdA BNILDI_10385 0.41 -0.2
2,091,997 + ppdA BNILDI_10385 0.54 +0.0
2,092,252 - +0.4
2,092,268 + BNILDI_10390 0.11 -0.1
2,092,269 - BNILDI_10390 0.12 -1.0
2,092,458 + BNILDI_10390 0.45 -0.5
2,092,650 - BNILDI_10390 0.79 -0.3
2,092,692 + BNILDI_10390 0.87 -1.0
2,092,707 + BNILDI_10390 0.89 +0.0
2,092,741 + -1.5
2,092,742 - -0.2
2,092,743 + -0.2
2,092,984 - ygdB BNILDI_10395 0.54 -1.0
2,093,018 - ygdB BNILDI_10395 0.62 -1.2
2,093,155 + +0.0
2,093,155 + -0.5
2,093,155 + +1.8

Or see this region's nucleotide sequence