Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lgt and thyA are separated by 6 nucleotides thyA and ppdA are separated by 184 nucleotides ppdA and BNILDI_10390 overlap by 10 nucleotides BNILDI_10390 and ygdB overlap by 4 nucleotides ygdB and BNILDI_10400 overlap by 16 nucleotides
BNILDI_10375: lgt - prolipoprotein diacylglyceryl transferase, at 2,089,882 to 2,090,757
lgt
BNILDI_10380: thyA - thymidylate synthase, at 2,090,764 to 2,091,558
thyA
BNILDI_10385: ppdA - prepilin peptidase-dependent protein, at 2,091,743 to 2,092,213
ppdA
BNILDI_10390: BNILDI_10390 - Prepilin peptidase dependent protein B, at 2,092,204 to 2,092,767
_10390
BNILDI_10395: ygdB - DUF2509 domain-containing protein, at 2,092,764 to 2,093,171
ygdB
BNILDI_10400: BNILDI_10400 - DUF2509 domain-containing protein, at 2,093,156 to 2,093,479
_10400
Position (kb)
2091
2092
2093 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2091.556 kb on + strand at 2091.556 kb on + strand at 2091.557 kb on - strand at 2091.557 kb on - strand at 2091.557 kb on - strand at 2091.667 kb on + strand at 2091.696 kb on + strand at 2091.763 kb on + strand at 2091.763 kb on + strand at 2091.763 kb on + strand at 2091.764 kb on - strand at 2091.825 kb on - strand, within ppdA at 2091.887 kb on + strand, within ppdA at 2091.896 kb on + strand, within ppdA at 2091.896 kb on + strand, within ppdA at 2091.897 kb on - strand, within ppdA at 2091.901 kb on - strand, within ppdA at 2091.937 kb on + strand, within ppdA at 2091.938 kb on - strand, within ppdA at 2091.997 kb on + strand, within ppdA at 2092.252 kb on - strand at 2092.268 kb on + strand, within BNILDI_10390 at 2092.269 kb on - strand, within BNILDI_10390 at 2092.458 kb on + strand, within BNILDI_10390 at 2092.650 kb on - strand, within BNILDI_10390 at 2092.692 kb on + strand, within BNILDI_10390 at 2092.707 kb on + strand, within BNILDI_10390 at 2092.741 kb on + strand at 2092.742 kb on - strand at 2092.743 kb on + strand at 2092.984 kb on - strand, within ygdB at 2093.018 kb on - strand, within ygdB at 2093.155 kb on + strand at 2093.155 kb on + strand at 2093.155 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 2,091,556 + +1.8 2,091,556 + +0.6 2,091,557 - -0.3 2,091,557 - -0.9 2,091,557 - +1.5 2,091,667 + +0.3 2,091,696 + +0.1 2,091,763 + +0.1 2,091,763 + -0.3 2,091,763 + -0.5 2,091,764 - -0.3 2,091,825 - ppdA BNILDI_10385 0.17 -1.5 2,091,887 + ppdA BNILDI_10385 0.31 +0.3 2,091,896 + ppdA BNILDI_10385 0.32 +0.0 2,091,896 + ppdA BNILDI_10385 0.32 -1.4 2,091,897 - ppdA BNILDI_10385 0.33 -1.1 2,091,901 - ppdA BNILDI_10385 0.34 -2.1 2,091,937 + ppdA BNILDI_10385 0.41 +0.3 2,091,938 - ppdA BNILDI_10385 0.41 -0.2 2,091,997 + ppdA BNILDI_10385 0.54 +0.0 2,092,252 - +0.4 2,092,268 + BNILDI_10390 0.11 -0.1 2,092,269 - BNILDI_10390 0.12 -1.0 2,092,458 + BNILDI_10390 0.45 -0.5 2,092,650 - BNILDI_10390 0.79 -0.3 2,092,692 + BNILDI_10390 0.87 -1.0 2,092,707 + BNILDI_10390 0.89 +0.0 2,092,741 + -1.5 2,092,742 - -0.2 2,092,743 + -0.2 2,092,984 - ygdB BNILDI_10395 0.54 -1.0 2,093,018 - ygdB BNILDI_10395 0.62 -1.2 2,093,155 + +0.0 2,093,155 + -0.5 2,093,155 + +1.8
Or see this region's nucleotide sequence