Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queG and nnr are separated by 13 nucleotides nnr and tsaE overlap by 29 nucleotides tsaE and amiB are separated by 18 nucleotides
BNILDI_06940: queG - tRNA epoxyqueuosine(34) reductase QueG, at 1,387,670 to 1,388,809
queG
BNILDI_06945: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 1,388,823 to 1,390,355
nnr
BNILDI_06950: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,390,327 to 1,390,788
tsaE
BNILDI_06955: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 1,390,807 to 1,392,144
amiB
Position (kb)
1388
1389
1390
1391 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1387.868 kb on + strand, within queG at 1387.882 kb on + strand, within queG at 1387.883 kb on - strand, within queG at 1387.909 kb on + strand, within queG at 1387.967 kb on + strand, within queG at 1387.967 kb on + strand, within queG at 1387.992 kb on - strand, within queG at 1388.057 kb on - strand, within queG at 1388.057 kb on - strand, within queG at 1388.079 kb on + strand, within queG at 1388.079 kb on + strand, within queG at 1388.084 kb on - strand, within queG at 1388.146 kb on - strand, within queG at 1388.152 kb on + strand, within queG at 1388.152 kb on + strand, within queG at 1388.153 kb on - strand, within queG at 1388.173 kb on + strand, within queG at 1388.173 kb on + strand, within queG at 1388.173 kb on + strand, within queG at 1388.173 kb on + strand, within queG at 1388.336 kb on - strand, within queG at 1388.511 kb on + strand, within queG at 1388.511 kb on + strand, within queG at 1388.566 kb on - strand, within queG at 1388.725 kb on + strand at 1388.725 kb on + strand at 1388.728 kb on + strand at 1388.728 kb on + strand at 1388.729 kb on - strand at 1388.732 kb on + strand at 1388.732 kb on + strand at 1388.732 kb on + strand at 1388.732 kb on + strand at 1388.733 kb on - strand at 1388.733 kb on - strand at 1388.819 kb on + strand at 1388.819 kb on + strand at 1388.820 kb on - strand at 1388.820 kb on - strand at 1388.820 kb on - strand at 1388.843 kb on + strand at 1388.843 kb on + strand at 1388.844 kb on - strand at 1388.845 kb on + strand at 1388.915 kb on + strand at 1388.916 kb on - strand at 1388.916 kb on - strand at 1388.916 kb on - strand at 1388.922 kb on + strand at 1388.923 kb on - strand at 1389.145 kb on + strand, within nnr at 1389.176 kb on + strand, within nnr at 1389.278 kb on + strand, within nnr at 1389.621 kb on - strand, within nnr at 1389.640 kb on + strand, within nnr at 1389.750 kb on - strand, within nnr at 1389.955 kb on + strand, within nnr at 1389.955 kb on + strand, within nnr at 1389.956 kb on - strand, within nnr at 1389.992 kb on - strand, within nnr at 1390.012 kb on + strand, within nnr at 1390.145 kb on + strand, within nnr at 1390.182 kb on + strand, within nnr at 1390.183 kb on - strand, within nnr at 1390.288 kb on + strand at 1390.814 kb on - strand at 1390.899 kb on + strand at 1390.951 kb on + strand, within amiB at 1390.952 kb on - strand, within amiB at 1390.952 kb on - strand, within amiB at 1390.952 kb on - strand, within amiB at 1391.161 kb on + strand, within amiB at 1391.161 kb on + strand, within amiB at 1391.161 kb on + strand, within amiB at 1391.162 kb on - strand, within amiB at 1391.162 kb on - strand, within amiB at 1391.162 kb on - strand, within amiB at 1391.162 kb on - strand, within amiB at 1391.302 kb on - strand, within amiB at 1391.319 kb on + strand, within amiB at 1391.332 kb on - strand, within amiB
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 1,387,868 + queG BNILDI_06940 0.17 +1.3 1,387,882 + queG BNILDI_06940 0.19 +0.6 1,387,883 - queG BNILDI_06940 0.19 -0.1 1,387,909 + queG BNILDI_06940 0.21 -1.9 1,387,967 + queG BNILDI_06940 0.26 +0.2 1,387,967 + queG BNILDI_06940 0.26 +0.9 1,387,992 - queG BNILDI_06940 0.28 -0.2 1,388,057 - queG BNILDI_06940 0.34 -0.7 1,388,057 - queG BNILDI_06940 0.34 -0.1 1,388,079 + queG BNILDI_06940 0.36 +1.2 1,388,079 + queG BNILDI_06940 0.36 +0.4 1,388,084 - queG BNILDI_06940 0.36 -0.1 1,388,146 - queG BNILDI_06940 0.42 -2.3 1,388,152 + queG BNILDI_06940 0.42 -1.5 1,388,152 + queG BNILDI_06940 0.42 -1.3 1,388,153 - queG BNILDI_06940 0.42 +0.9 1,388,173 + queG BNILDI_06940 0.44 +4.5 1,388,173 + queG BNILDI_06940 0.44 +0.1 1,388,173 + queG BNILDI_06940 0.44 -2.4 1,388,173 + queG BNILDI_06940 0.44 +0.9 1,388,336 - queG BNILDI_06940 0.58 +1.3 1,388,511 + queG BNILDI_06940 0.74 +0.0 1,388,511 + queG BNILDI_06940 0.74 +0.8 1,388,566 - queG BNILDI_06940 0.79 +1.3 1,388,725 + -0.1 1,388,725 + +0.9 1,388,728 + -1.0 1,388,728 + -0.5 1,388,729 - -0.4 1,388,732 + -1.5 1,388,732 + -0.1 1,388,732 + +0.1 1,388,732 + -0.7 1,388,733 - -2.0 1,388,733 - +0.3 1,388,819 + -1.2 1,388,819 + -0.8 1,388,820 - -1.3 1,388,820 - -0.4 1,388,820 - +0.1 1,388,843 + -2.2 1,388,843 + +1.4 1,388,844 - -2.5 1,388,845 + -0.2 1,388,915 + -0.3 1,388,916 - -0.3 1,388,916 - +0.3 1,388,916 - -2.2 1,388,922 + +0.0 1,388,923 - -0.5 1,389,145 + nnr BNILDI_06945 0.21 -1.1 1,389,176 + nnr BNILDI_06945 0.23 +0.9 1,389,278 + nnr BNILDI_06945 0.30 -0.9 1,389,621 - nnr BNILDI_06945 0.52 +0.3 1,389,640 + nnr BNILDI_06945 0.53 +0.5 1,389,750 - nnr BNILDI_06945 0.60 -0.9 1,389,955 + nnr BNILDI_06945 0.74 +0.8 1,389,955 + nnr BNILDI_06945 0.74 +0.9 1,389,956 - nnr BNILDI_06945 0.74 -0.3 1,389,992 - nnr BNILDI_06945 0.76 +0.9 1,390,012 + nnr BNILDI_06945 0.78 -0.2 1,390,145 + nnr BNILDI_06945 0.86 -1.7 1,390,182 + nnr BNILDI_06945 0.89 -0.4 1,390,183 - nnr BNILDI_06945 0.89 -2.1 1,390,288 + +0.9 1,390,814 - -0.1 1,390,899 + -1.2 1,390,951 + amiB BNILDI_06955 0.11 -1.6 1,390,952 - amiB BNILDI_06955 0.11 -0.7 1,390,952 - amiB BNILDI_06955 0.11 -0.5 1,390,952 - amiB BNILDI_06955 0.11 +0.0 1,391,161 + amiB BNILDI_06955 0.26 -0.0 1,391,161 + amiB BNILDI_06955 0.26 -0.4 1,391,161 + amiB BNILDI_06955 0.26 -0.2 1,391,162 - amiB BNILDI_06955 0.27 -0.1 1,391,162 - amiB BNILDI_06955 0.27 -0.0 1,391,162 - amiB BNILDI_06955 0.27 +0.9 1,391,162 - amiB BNILDI_06955 0.27 -0.1 1,391,302 - amiB BNILDI_06955 0.37 -0.3 1,391,319 + amiB BNILDI_06955 0.38 -0.7 1,391,332 - amiB BNILDI_06955 0.39 +0.2
Or see this region's nucleotide sequence