Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcG and yfcF are separated by 135 nucleotides yfcF and yfcE are separated by 55 nucleotides yfcE and yfcD are separated by 57 nucleotides yfcD and yfcC are separated by 32 nucleotides
HEPCGN_21970: yfcG - GSH-dependent disulfide bond oxidoreductase, at 3,149,189 to 3,149,836
yfcG
HEPCGN_21975: yfcF - glutathione transferase, at 3,149,972 to 3,150,616
yfcF
HEPCGN_21980: yfcE - phosphodiesterase, at 3,150,672 to 3,151,223
yfcE
HEPCGN_21985: yfcD - NUDIX hydrolase YfcD, at 3,151,281 to 3,151,823
yfcD
HEPCGN_21990: yfcC - putative basic amino acid antiporter YfcC, at 3,151,856 to 3,153,334
yfcC
Position (kb)
3150
3151
3152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3149.823 kb on + strand at 3149.854 kb on - strand at 3149.875 kb on - strand at 3149.877 kb on - strand at 3149.934 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.971 kb on - strand at 3149.977 kb on + strand at 3149.978 kb on - strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.073 kb on + strand, within yfcF at 3150.156 kb on + strand, within yfcF at 3150.241 kb on + strand, within yfcF at 3150.242 kb on - strand, within yfcF at 3150.476 kb on - strand, within yfcF at 3150.510 kb on + strand, within yfcF at 3150.511 kb on - strand, within yfcF at 3150.641 kb on + strand at 3150.641 kb on + strand at 3150.809 kb on - strand, within yfcE at 3150.863 kb on + strand, within yfcE at 3150.863 kb on + strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.864 kb on - strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.949 kb on + strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3150.950 kb on - strand, within yfcE at 3151.112 kb on + strand, within yfcE at 3151.119 kb on - strand, within yfcE at 3151.119 kb on - strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.162 kb on + strand, within yfcE at 3151.163 kb on - strand, within yfcE at 3151.172 kb on - strand at 3151.172 kb on - strand at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.401 kb on + strand, within yfcD at 3151.402 kb on - strand, within yfcD at 3151.481 kb on - strand, within yfcD at 3151.802 kb on - strand at 3151.857 kb on + strand at 3151.903 kb on + strand at 3151.904 kb on - strand at 3151.906 kb on + strand at 3152.168 kb on - strand, within yfcC at 3152.168 kb on - strand, within yfcC
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 3,149,823 + +1.4 3,149,854 - +1.0 3,149,875 - +0.2 3,149,877 - +0.7 3,149,934 + -1.5 3,149,970 + -0.7 3,149,970 + +1.5 3,149,970 + -0.3 3,149,970 + +0.5 3,149,971 - -0.2 3,149,977 + -0.6 3,149,978 - -0.3 3,150,023 + +0.4 3,150,023 + -0.0 3,150,023 + +0.4 3,150,073 + yfcF HEPCGN_21975 0.16 -3.3 3,150,156 + yfcF HEPCGN_21975 0.29 +0.3 3,150,241 + yfcF HEPCGN_21975 0.42 +4.2 3,150,242 - yfcF HEPCGN_21975 0.42 +0.1 3,150,476 - yfcF HEPCGN_21975 0.78 -0.3 3,150,510 + yfcF HEPCGN_21975 0.83 -1.5 3,150,511 - yfcF HEPCGN_21975 0.84 -1.0 3,150,641 + -0.6 3,150,641 + +0.2 3,150,809 - yfcE HEPCGN_21980 0.25 +1.4 3,150,863 + yfcE HEPCGN_21980 0.35 -0.4 3,150,863 + yfcE HEPCGN_21980 0.35 +0.1 3,150,864 - yfcE HEPCGN_21980 0.35 +0.3 3,150,864 - yfcE HEPCGN_21980 0.35 +0.8 3,150,864 - yfcE HEPCGN_21980 0.35 -0.1 3,150,864 - yfcE HEPCGN_21980 0.35 +1.5 3,150,864 - yfcE HEPCGN_21980 0.35 +0.2 3,150,949 + yfcE HEPCGN_21980 0.50 +0.1 3,150,949 + yfcE HEPCGN_21980 0.50 +0.7 3,150,949 + yfcE HEPCGN_21980 0.50 -0.5 3,150,950 - yfcE HEPCGN_21980 0.50 +0.5 3,150,950 - yfcE HEPCGN_21980 0.50 +0.0 3,150,950 - yfcE HEPCGN_21980 0.50 +0.0 3,151,112 + yfcE HEPCGN_21980 0.80 +0.5 3,151,119 - yfcE HEPCGN_21980 0.81 -0.3 3,151,119 - yfcE HEPCGN_21980 0.81 +1.7 3,151,162 + yfcE HEPCGN_21980 0.89 +0.3 3,151,162 + yfcE HEPCGN_21980 0.89 +0.3 3,151,162 + yfcE HEPCGN_21980 0.89 +0.3 3,151,162 + yfcE HEPCGN_21980 0.89 +0.6 3,151,162 + yfcE HEPCGN_21980 0.89 -0.5 3,151,163 - yfcE HEPCGN_21980 0.89 +0.1 3,151,172 - +0.2 3,151,172 - -0.1 3,151,401 + yfcD HEPCGN_21985 0.22 +0.2 3,151,401 + yfcD HEPCGN_21985 0.22 +1.0 3,151,401 + yfcD HEPCGN_21985 0.22 +0.2 3,151,401 + yfcD HEPCGN_21985 0.22 +0.0 3,151,401 + yfcD HEPCGN_21985 0.22 -0.5 3,151,402 - yfcD HEPCGN_21985 0.22 -0.0 3,151,481 - yfcD HEPCGN_21985 0.37 -0.6 3,151,802 - -1.2 3,151,857 + -1.1 3,151,903 + -0.0 3,151,904 - -0.7 3,151,906 + +0.3 3,152,168 - yfcC HEPCGN_21990 0.21 -0.6 3,152,168 - yfcC HEPCGN_21990 0.21 -0.5
Or see this region's nucleotide sequence