Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuB and leuC1 are separated by 2 nucleotides leuC1 and leuD are separated by 10 nucleotides leuD and HEPCGN_08720 are separated by 107 nucleotides HEPCGN_08720 and sgrR are separated by 88 nucleotides
HEPCGN_08700: leuB - 3-isopropylmalate dehydrogenase, at 480,436 to 481,527
leuB
HEPCGN_08705: leuC1 - 3-isopropylmalate dehydratase large subunit 1, at 481,530 to 482,930
leuC1
HEPCGN_08710: leuD - 3-isopropylmalate dehydratase small subunit, at 482,941 to 483,546
leuD
HEPCGN_08720: HEPCGN_08720 - glucose uptake inhibitor SgrT, at 483,654 to 483,785
_08720
HEPCGN_08725: sgrR - DNA-binding transcriptional regulator SgrR, at 483,874 to 485,532
sgrR
Position (kb) 481
482
483 Strain fitness (log2 ratio) -1
0
1
2 at 480.563 kb on - strand, within leuB at 480.589 kb on - strand, within leuB at 480.663 kb on - strand, within leuB at 480.733 kb on - strand, within leuB at 480.935 kb on - strand, within leuB at 480.935 kb on - strand, within leuB at 480.935 kb on - strand, within leuB at 480.935 kb on - strand, within leuB at 481.011 kb on + strand, within leuB at 481.104 kb on + strand, within leuB at 481.104 kb on + strand, within leuB at 481.108 kb on + strand, within leuB at 481.109 kb on - strand, within leuB at 481.451 kb on + strand at 481.473 kb on + strand at 481.535 kb on + strand at 481.570 kb on + strand at 481.576 kb on - strand at 481.603 kb on + strand at 481.603 kb on + strand at 481.603 kb on + strand at 481.603 kb on + strand at 481.603 kb on + strand at 481.603 kb on + strand at 481.604 kb on - strand at 481.604 kb on - strand at 481.604 kb on - strand at 481.605 kb on + strand at 481.821 kb on + strand, within leuC1 at 481.858 kb on + strand, within leuC1 at 481.858 kb on + strand, within leuC1 at 481.858 kb on + strand, within leuC1 at 481.858 kb on + strand, within leuC1 at 481.859 kb on - strand, within leuC1 at 481.984 kb on + strand, within leuC1 at 481.984 kb on + strand, within leuC1 at 481.985 kb on - strand, within leuC1 at 482.076 kb on - strand, within leuC1 at 482.162 kb on - strand, within leuC1 at 482.177 kb on + strand, within leuC1 at 482.193 kb on - strand, within leuC1 at 482.249 kb on + strand, within leuC1 at 482.250 kb on - strand, within leuC1 at 482.250 kb on - strand, within leuC1 at 482.250 kb on - strand, within leuC1 at 482.250 kb on - strand, within leuC1 at 482.253 kb on + strand, within leuC1 at 482.253 kb on + strand, within leuC1 at 482.253 kb on + strand, within leuC1 at 482.438 kb on + strand, within leuC1 at 482.439 kb on - strand, within leuC1 at 482.502 kb on + strand, within leuC1 at 482.674 kb on + strand, within leuC1 at 482.924 kb on + strand at 482.924 kb on + strand at 482.964 kb on + strand at 482.965 kb on - strand at 482.965 kb on - strand at 483.142 kb on + strand, within leuD at 483.143 kb on - strand, within leuD at 483.235 kb on - strand, within leuD at 483.514 kb on + strand at 483.537 kb on + strand at 483.544 kb on + strand at 483.552 kb on - strand at 483.596 kb on + strand at 483.597 kb on - strand at 483.627 kb on + strand at 483.627 kb on + strand at 483.701 kb on + strand, within HEPCGN_08720 at 483.702 kb on - strand, within HEPCGN_08720 at 483.705 kb on + strand, within HEPCGN_08720 at 483.764 kb on - strand, within HEPCGN_08720 at 483.824 kb on + strand at 483.825 kb on - strand at 483.825 kb on - strand at 483.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 480,563 - leuB HEPCGN_08700 0.12 -1.0 480,589 - leuB HEPCGN_08700 0.14 +0.6 480,663 - leuB HEPCGN_08700 0.21 +0.0 480,733 - leuB HEPCGN_08700 0.27 -0.3 480,935 - leuB HEPCGN_08700 0.46 -0.2 480,935 - leuB HEPCGN_08700 0.46 -0.2 480,935 - leuB HEPCGN_08700 0.46 +0.3 480,935 - leuB HEPCGN_08700 0.46 +0.2 481,011 + leuB HEPCGN_08700 0.53 +0.7 481,104 + leuB HEPCGN_08700 0.61 -0.3 481,104 + leuB HEPCGN_08700 0.61 +0.3 481,108 + leuB HEPCGN_08700 0.62 +0.5 481,109 - leuB HEPCGN_08700 0.62 +0.2 481,451 + +1.0 481,473 + -1.3 481,535 + -0.4 481,570 + +0.3 481,576 - -0.3 481,603 + -0.2 481,603 + +0.7 481,603 + +0.2 481,603 + +1.4 481,603 + -0.2 481,603 + +0.1 481,604 - -0.5 481,604 - -0.1 481,604 - -0.6 481,605 + +0.4 481,821 + leuC1 HEPCGN_08705 0.21 -0.0 481,858 + leuC1 HEPCGN_08705 0.23 +0.2 481,858 + leuC1 HEPCGN_08705 0.23 -0.1 481,858 + leuC1 HEPCGN_08705 0.23 +2.0 481,858 + leuC1 HEPCGN_08705 0.23 +0.0 481,859 - leuC1 HEPCGN_08705 0.23 +0.8 481,984 + leuC1 HEPCGN_08705 0.32 +0.7 481,984 + leuC1 HEPCGN_08705 0.32 -0.2 481,985 - leuC1 HEPCGN_08705 0.32 +0.4 482,076 - leuC1 HEPCGN_08705 0.39 -0.5 482,162 - leuC1 HEPCGN_08705 0.45 +0.5 482,177 + leuC1 HEPCGN_08705 0.46 +1.1 482,193 - leuC1 HEPCGN_08705 0.47 +1.1 482,249 + leuC1 HEPCGN_08705 0.51 -0.4 482,250 - leuC1 HEPCGN_08705 0.51 -0.4 482,250 - leuC1 HEPCGN_08705 0.51 -0.3 482,250 - leuC1 HEPCGN_08705 0.51 -0.3 482,250 - leuC1 HEPCGN_08705 0.51 +0.4 482,253 + leuC1 HEPCGN_08705 0.52 -1.0 482,253 + leuC1 HEPCGN_08705 0.52 -0.1 482,253 + leuC1 HEPCGN_08705 0.52 -0.3 482,438 + leuC1 HEPCGN_08705 0.65 +0.5 482,439 - leuC1 HEPCGN_08705 0.65 -0.0 482,502 + leuC1 HEPCGN_08705 0.69 +0.1 482,674 + leuC1 HEPCGN_08705 0.82 +2.3 482,924 + -0.3 482,924 + +0.7 482,964 + +1.2 482,965 - -0.5 482,965 - +0.4 483,142 + leuD HEPCGN_08710 0.33 +0.0 483,143 - leuD HEPCGN_08710 0.33 -0.3 483,235 - leuD HEPCGN_08710 0.49 -0.9 483,514 + +0.3 483,537 + +1.6 483,544 + -0.1 483,552 - -0.1 483,596 + +0.6 483,597 - -1.2 483,627 + +0.3 483,627 + -0.3 483,701 + HEPCGN_08720 0.36 +0.6 483,702 - HEPCGN_08720 0.36 -0.3 483,705 + HEPCGN_08720 0.39 +0.5 483,764 - HEPCGN_08720 0.83 -0.4 483,824 + +0.9 483,825 - +0.5 483,825 - -1.0 483,825 - -0.2
Or see this region's nucleotide sequence