Strain Fitness in Escherichia coli ECOR38 around HEPCGN_08010

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmetQ and rcsF are separated by 117 nucleotidesrcsF and tsaA overlap by 4 nucleotidestsaA and proS are separated by 110 nucleotides HEPCGN_08000: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 336,035 to 336,850 metQ HEPCGN_08005: rcsF - Rcs stress response system protein RcsF, at 336,968 to 337,372 rcsF HEPCGN_08010: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 337,369 to 338,076 tsaA HEPCGN_08015: proS - proline--tRNA ligase, at 338,187 to 339,905 proS Position (kb) 337 338 339Strain fitness (log2 ratio) -1 0 1at 336.383 kb on + strand, within metQat 336.647 kb on + strand, within metQat 336.647 kb on + strand, within metQat 336.647 kb on + strand, within metQat 336.647 kb on + strand, within metQat 336.648 kb on - strand, within metQat 336.648 kb on - strand, within metQat 336.648 kb on - strand, within metQat 336.648 kb on - strand, within metQat 336.686 kb on - strand, within metQat 336.700 kb on + strand, within metQat 336.701 kb on - strand, within metQat 336.782 kb on + strandat 336.823 kb on + strandat 336.824 kb on - strandat 336.851 kb on + strandat 336.856 kb on - strandat 336.879 kb on - strandat 336.935 kb on - strandat 336.965 kb on - strandat 336.967 kb on + strandat 336.968 kb on - strandat 336.968 kb on - strandat 336.994 kb on - strandat 337.024 kb on - strand, within rcsFat 337.121 kb on + strand, within rcsFat 337.234 kb on + strand, within rcsFat 337.278 kb on + strand, within rcsFat 337.282 kb on - strand, within rcsFat 337.288 kb on + strand, within rcsFat 337.289 kb on - strand, within rcsFat 337.310 kb on - strand, within rcsFat 337.336 kb on - strandat 337.336 kb on - strandat 337.402 kb on - strandat 337.414 kb on + strandat 337.455 kb on - strand, within tsaAat 337.475 kb on + strand, within tsaAat 337.475 kb on + strand, within tsaAat 337.476 kb on - strand, within tsaAat 337.493 kb on - strand, within tsaAat 337.493 kb on - strand, within tsaAat 337.538 kb on + strand, within tsaAat 337.538 kb on + strand, within tsaAat 337.671 kb on + strand, within tsaAat 337.671 kb on + strand, within tsaAat 337.672 kb on - strand, within tsaAat 337.672 kb on - strand, within tsaAat 337.755 kb on + strand, within tsaAat 337.780 kb on + strand, within tsaAat 337.781 kb on - strand, within tsaAat 337.781 kb on - strand, within tsaAat 337.822 kb on + strand, within tsaAat 337.822 kb on + strand, within tsaAat 337.823 kb on - strand, within tsaAat 337.917 kb on - strand, within tsaAat 337.917 kb on - strand, within tsaAat 337.937 kb on + strand, within tsaAat 337.968 kb on + strand, within tsaAat 337.969 kb on - strand, within tsaAat 337.969 kb on - strand, within tsaAat 337.969 kb on - strand, within tsaAat 338.017 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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336,383 + metQ HEPCGN_08000 0.43 -0.2
336,647 + metQ HEPCGN_08000 0.75 +0.4
336,647 + metQ HEPCGN_08000 0.75 -0.1
336,647 + metQ HEPCGN_08000 0.75 -0.4
336,647 + metQ HEPCGN_08000 0.75 -0.4
336,648 - metQ HEPCGN_08000 0.75 +0.6
336,648 - metQ HEPCGN_08000 0.75 -0.3
336,648 - metQ HEPCGN_08000 0.75 -0.6
336,648 - metQ HEPCGN_08000 0.75 -0.3
336,686 - metQ HEPCGN_08000 0.80 -0.6
336,700 + metQ HEPCGN_08000 0.81 -0.7
336,701 - metQ HEPCGN_08000 0.82 -0.4
336,782 + -0.8
336,823 + -0.9
336,824 - +0.1
336,851 + -0.4
336,856 - +0.2
336,879 - -0.4
336,935 - +0.2
336,965 - +0.4
336,967 + +1.5
336,968 - -0.4
336,968 - +0.4
336,994 - +0.0
337,024 - rcsF HEPCGN_08005 0.14 -0.2
337,121 + rcsF HEPCGN_08005 0.38 +1.3
337,234 + rcsF HEPCGN_08005 0.66 +0.9
337,278 + rcsF HEPCGN_08005 0.77 +1.0
337,282 - rcsF HEPCGN_08005 0.78 +1.0
337,288 + rcsF HEPCGN_08005 0.79 -0.0
337,289 - rcsF HEPCGN_08005 0.79 -0.1
337,310 - rcsF HEPCGN_08005 0.84 +0.4
337,336 - +0.4
337,336 - +0.5
337,402 - -0.5
337,414 + +0.8
337,455 - tsaA HEPCGN_08010 0.12 +0.4
337,475 + tsaA HEPCGN_08010 0.15 -0.2
337,475 + tsaA HEPCGN_08010 0.15 -0.2
337,476 - tsaA HEPCGN_08010 0.15 +0.4
337,493 - tsaA HEPCGN_08010 0.18 -0.2
337,493 - tsaA HEPCGN_08010 0.18 +0.1
337,538 + tsaA HEPCGN_08010 0.24 +0.7
337,538 + tsaA HEPCGN_08010 0.24 +0.6
337,671 + tsaA HEPCGN_08010 0.43 -0.4
337,671 + tsaA HEPCGN_08010 0.43 -0.5
337,672 - tsaA HEPCGN_08010 0.43 -0.2
337,672 - tsaA HEPCGN_08010 0.43 -0.5
337,755 + tsaA HEPCGN_08010 0.55 -0.4
337,780 + tsaA HEPCGN_08010 0.58 +0.0
337,781 - tsaA HEPCGN_08010 0.58 +0.3
337,781 - tsaA HEPCGN_08010 0.58 +1.0
337,822 + tsaA HEPCGN_08010 0.64 -0.2
337,822 + tsaA HEPCGN_08010 0.64 +0.4
337,823 - tsaA HEPCGN_08010 0.64 -0.6
337,917 - tsaA HEPCGN_08010 0.77 -0.4
337,917 - tsaA HEPCGN_08010 0.77 +0.2
337,937 + tsaA HEPCGN_08010 0.80 -0.5
337,968 + tsaA HEPCGN_08010 0.85 +1.0
337,969 - tsaA HEPCGN_08010 0.85 +0.5
337,969 - tsaA HEPCGN_08010 0.85 +0.6
337,969 - tsaA HEPCGN_08010 0.85 +0.1
338,017 - +1.2

Or see this region's nucleotide sequence