Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF925 and GFF926 are separated by 46 nucleotides GFF926 and GFF927 overlap by 4 nucleotides GFF927 and GFF928 are separated by 155 nucleotides
GFF925 - hypothetical protein, at 237,279 to 238,058
GFF925
GFF926 - Transcriptional regulator, AcrR family, at 238,105 to 238,785
GFF926
GFF927 - Acyl-CoA dehydrogenase, at 238,782 to 240,494
GFF927
GFF928 - tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.91), at 240,650 to 241,630
GFF928
Position (kb)
238
239
240
241 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 237.867 kb on + strand, within GFF925 at 237.867 kb on + strand, within GFF925 at 237.867 kb on + strand, within GFF925 at 237.867 kb on + strand, within GFF925 at 237.867 kb on + strand, within GFF925 at 237.868 kb on - strand, within GFF925 at 237.868 kb on - strand, within GFF925 at 237.868 kb on - strand, within GFF925 at 237.868 kb on - strand, within GFF925 at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.014 kb on + strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.015 kb on - strand at 238.111 kb on + strand at 238.112 kb on - strand at 238.112 kb on - strand at 238.261 kb on + strand, within GFF926 at 238.262 kb on - strand, within GFF926 at 238.262 kb on - strand, within GFF926 at 238.262 kb on - strand, within GFF926 at 238.262 kb on - strand, within GFF926 at 238.267 kb on + strand, within GFF926 at 238.267 kb on + strand, within GFF926 at 238.267 kb on + strand, within GFF926 at 238.268 kb on - strand, within GFF926 at 238.436 kb on - strand, within GFF926 at 238.489 kb on + strand, within GFF926 at 238.489 kb on + strand, within GFF926 at 238.514 kb on + strand, within GFF926 at 238.514 kb on + strand, within GFF926 at 238.515 kb on - strand, within GFF926 at 238.669 kb on + strand, within GFF926 at 238.669 kb on + strand, within GFF926 at 238.669 kb on + strand, within GFF926 at 238.669 kb on + strand, within GFF926 at 238.670 kb on - strand, within GFF926 at 238.830 kb on + strand at 238.830 kb on + strand at 238.830 kb on + strand at 238.831 kb on - strand at 239.181 kb on + strand, within GFF927 at 239.181 kb on + strand, within GFF927 at 239.181 kb on + strand, within GFF927 at 239.181 kb on + strand, within GFF927 at 239.182 kb on - strand, within GFF927 at 239.232 kb on + strand, within GFF927 at 239.232 kb on + strand, within GFF927 at 239.232 kb on + strand, within GFF927 at 239.233 kb on - strand, within GFF927 at 239.233 kb on - strand, within GFF927 at 239.233 kb on - strand, within GFF927 at 239.233 kb on - strand, within GFF927 at 239.233 kb on - strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.304 kb on + strand, within GFF927 at 239.305 kb on - strand, within GFF927 at 239.305 kb on - strand, within GFF927 at 239.356 kb on - strand, within GFF927 at 239.434 kb on - strand, within GFF927 at 239.458 kb on - strand, within GFF927 at 239.458 kb on - strand, within GFF927 at 239.652 kb on + strand, within GFF927 at 239.652 kb on + strand, within GFF927 at 239.652 kb on + strand, within GFF927 at 239.653 kb on - strand, within GFF927 at 239.653 kb on - strand, within GFF927 at 239.653 kb on - strand, within GFF927 at 240.561 kb on + strand at 240.562 kb on + strand at 240.563 kb on - strand at 240.563 kb on - strand at 240.563 kb on - strand at 240.563 kb on - strand at 240.563 kb on - strand at 240.563 kb on - strand at 240.628 kb on + strand at 240.632 kb on + strand at 240.636 kb on - strand at 240.637 kb on + strand at 240.637 kb on + strand at 240.637 kb on + strand at 240.638 kb on - strand at 240.638 kb on - strand at 240.638 kb on - strand at 240.638 kb on - strand at 240.643 kb on + strand at 240.643 kb on + strand at 240.643 kb on + strand at 240.643 kb on + strand at 240.643 kb on + strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.644 kb on - strand at 240.716 kb on + strand at 240.717 kb on - strand at 240.717 kb on - strand at 240.717 kb on - strand at 240.752 kb on + strand, within GFF928 at 240.752 kb on + strand, within GFF928 at 240.752 kb on + strand, within GFF928 at 240.752 kb on + strand, within GFF928 at 240.753 kb on - strand, within GFF928 at 240.753 kb on - strand, within GFF928 at 240.753 kb on - strand, within GFF928 at 240.753 kb on - strand, within GFF928 at 240.783 kb on + strand, within GFF928 at 240.784 kb on - strand, within GFF928 at 240.784 kb on - strand, within GFF928 at 240.784 kb on - strand, within GFF928 at 240.795 kb on + strand, within GFF928 at 240.795 kb on + strand, within GFF928 at 240.796 kb on - strand, within GFF928 at 240.796 kb on - strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.905 kb on + strand, within GFF928 at 240.906 kb on - strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.082 kb on + strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.083 kb on - strand, within GFF928 at 241.211 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.343 kb on + strand, within GFF928 at 241.344 kb on - strand, within GFF928 at 241.344 kb on - strand, within GFF928 at 241.385 kb on + strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.386 kb on - strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.442 kb on + strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928 at 241.443 kb on - strand, within GFF928
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 237,867 + GFF925 0.75 +0.6 237,867 + GFF925 0.75 -0.4 237,867 + GFF925 0.75 +0.4 237,867 + GFF925 0.75 +0.2 237,867 + GFF925 0.75 -0.2 237,868 - GFF925 0.76 +1.1 237,868 - GFF925 0.76 +0.3 237,868 - GFF925 0.76 -0.2 237,868 - GFF925 0.76 -1.0 238,014 + +0.6 238,014 + +0.3 238,014 + -1.0 238,014 + -0.5 238,014 + -0.9 238,014 + +0.6 238,014 + +0.3 238,014 + +0.2 238,014 + +0.6 238,014 + +0.5 238,014 + +0.2 238,014 + -0.9 238,014 + +1.0 238,014 + -0.4 238,014 + +1.0 238,014 + -0.4 238,014 + -0.0 238,014 + -0.4 238,015 - -0.6 238,015 - -1.2 238,015 - +0.7 238,015 - -0.2 238,015 - -0.2 238,015 - +0.1 238,015 - +0.2 238,015 - +0.8 238,015 - -1.5 238,015 - -0.3 238,015 - -0.2 238,015 - +0.2 238,015 - -0.2 238,111 + -0.1 238,112 - +0.6 238,112 - -1.2 238,261 + GFF926 0.23 +0.1 238,262 - GFF926 0.23 -0.6 238,262 - GFF926 0.23 +0.1 238,262 - GFF926 0.23 -0.8 238,262 - GFF926 0.23 -0.0 238,267 + GFF926 0.24 +0.6 238,267 + GFF926 0.24 -0.1 238,267 + GFF926 0.24 +1.2 238,268 - GFF926 0.24 -0.3 238,436 - GFF926 0.49 -0.6 238,489 + GFF926 0.56 -1.2 238,489 + GFF926 0.56 +2.4 238,514 + GFF926 0.60 -0.5 238,514 + GFF926 0.60 -1.2 238,515 - GFF926 0.60 -0.7 238,669 + GFF926 0.83 +1.0 238,669 + GFF926 0.83 -0.2 238,669 + GFF926 0.83 +0.4 238,669 + GFF926 0.83 -0.4 238,670 - GFF926 0.83 +0.4 238,830 + -1.0 238,830 + +0.1 238,830 + -0.5 238,831 - -0.9 239,181 + GFF927 0.23 +1.4 239,181 + GFF927 0.23 +0.6 239,181 + GFF927 0.23 -0.7 239,181 + GFF927 0.23 -1.0 239,182 - GFF927 0.23 -0.5 239,232 + GFF927 0.26 +0.7 239,232 + GFF927 0.26 -0.1 239,232 + GFF927 0.26 -0.5 239,233 - GFF927 0.26 +0.5 239,233 - GFF927 0.26 -0.4 239,233 - GFF927 0.26 -0.0 239,233 - GFF927 0.26 +1.4 239,233 - GFF927 0.26 +0.0 239,304 + GFF927 0.30 +0.0 239,304 + GFF927 0.30 -0.6 239,304 + GFF927 0.30 +0.0 239,304 + GFF927 0.30 +0.5 239,304 + GFF927 0.30 -0.1 239,304 + GFF927 0.30 +0.5 239,304 + GFF927 0.30 -0.2 239,305 - GFF927 0.31 -0.3 239,305 - GFF927 0.31 +0.0 239,356 - GFF927 0.34 +0.1 239,434 - GFF927 0.38 -0.3 239,458 - GFF927 0.39 +0.1 239,458 - GFF927 0.39 +0.4 239,652 + GFF927 0.51 -0.6 239,652 + GFF927 0.51 +0.0 239,652 + GFF927 0.51 -0.1 239,653 - GFF927 0.51 +2.1 239,653 - GFF927 0.51 +0.1 239,653 - GFF927 0.51 +2.4 240,561 + +0.5 240,562 + -0.6 240,563 - -0.5 240,563 - +0.6 240,563 - -0.4 240,563 - +0.2 240,563 - -0.6 240,563 - -1.4 240,628 + -2.6 240,632 + -2.0 240,636 - +0.5 240,637 + -0.1 240,637 + -0.5 240,637 + -0.2 240,638 - +0.5 240,638 - -0.4 240,638 - -0.9 240,638 - +0.8 240,643 + -1.0 240,643 + -0.6 240,643 + -0.7 240,643 + -0.0 240,643 + -0.7 240,644 - -0.5 240,644 - +0.0 240,644 - -1.0 240,644 - -0.2 240,644 - -0.6 240,644 - -0.2 240,644 - -0.5 240,716 + -1.4 240,717 - +0.1 240,717 - +0.1 240,717 - +0.8 240,752 + GFF928 0.10 +0.0 240,752 + GFF928 0.10 +0.9 240,752 + GFF928 0.10 +0.0 240,752 + GFF928 0.10 -1.0 240,753 - GFF928 0.10 -0.3 240,753 - GFF928 0.10 +1.1 240,753 - GFF928 0.10 -0.6 240,753 - GFF928 0.10 -0.2 240,783 + GFF928 0.14 +0.1 240,784 - GFF928 0.14 +0.5 240,784 - GFF928 0.14 +0.5 240,784 - GFF928 0.14 +0.2 240,795 + GFF928 0.15 +0.1 240,795 + GFF928 0.15 +0.6 240,796 - GFF928 0.15 -0.2 240,796 - GFF928 0.15 +0.3 240,905 + GFF928 0.26 -1.5 240,905 + GFF928 0.26 -2.4 240,905 + GFF928 0.26 -0.1 240,906 - GFF928 0.26 -2.6 241,082 + GFF928 0.44 -0.5 241,082 + GFF928 0.44 -0.6 241,082 + GFF928 0.44 +1.1 241,082 + GFF928 0.44 -0.4 241,082 + GFF928 0.44 -0.4 241,082 + GFF928 0.44 -0.0 241,082 + GFF928 0.44 +0.7 241,082 + GFF928 0.44 +0.5 241,083 - GFF928 0.44 -0.1 241,083 - GFF928 0.44 -0.8 241,083 - GFF928 0.44 +0.7 241,211 + GFF928 0.57 -0.2 241,343 + GFF928 0.71 -1.6 241,343 + GFF928 0.71 -0.3 241,343 + GFF928 0.71 -2.0 241,343 + GFF928 0.71 -0.8 241,344 - GFF928 0.71 -1.0 241,344 - GFF928 0.71 -0.3 241,385 + GFF928 0.75 -0.4 241,386 - GFF928 0.75 -0.6 241,386 - GFF928 0.75 -0.6 241,386 - GFF928 0.75 +0.8 241,442 + GFF928 0.81 -0.9 241,442 + GFF928 0.81 +0.2 241,442 + GFF928 0.81 -0.8 241,442 + GFF928 0.81 -0.2 241,442 + GFF928 0.81 -1.2 241,442 + GFF928 0.81 -1.3 241,442 + GFF928 0.81 +0.8 241,443 - GFF928 0.81 -0.4 241,443 - GFF928 0.81 -1.0 241,443 - GFF928 0.81 +0.1 241,443 - GFF928 0.81 +1.4 241,443 - GFF928 0.81 -0.2 241,443 - GFF928 0.81 -2.3 241,443 - GFF928 0.81 +2.2 241,443 - GFF928 0.81 -1.9 241,443 - GFF928 0.81 -1.5 241,443 - GFF928 0.81 +0.2
Or see this region's nucleotide sequence