Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF886 and GFF887 overlap by 4 nucleotides GFF887 and GFF888 are separated by 49 nucleotides GFF888 and GFF889 are separated by 62 nucleotides
GFF886 - Protein of unknown function DUF1446, at 195,275 to 196,699
GFF886
GFF887 - Small uncharacterized protein Bpro_4170, at 196,696 to 197,064
GFF887
GFF888 - BUG/TctC family periplasmic protein, at 197,114 to 198,079
GFF888
GFF889 - FIG00349738: hypothetical protein, at 198,142 to 200,244
GFF889
Position (kb)
197
198
199 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 196.136 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.271 kb on + strand, within GFF886 at 196.272 kb on - strand, within GFF886 at 196.272 kb on - strand, within GFF886 at 196.326 kb on - strand, within GFF886 at 196.565 kb on + strand at 196.565 kb on + strand at 196.565 kb on + strand at 196.886 kb on + strand, within GFF887 at 196.886 kb on + strand, within GFF887 at 196.886 kb on + strand, within GFF887 at 196.887 kb on - strand, within GFF887 at 196.887 kb on - strand, within GFF887 at 197.370 kb on - strand, within GFF888 at 197.567 kb on + strand, within GFF888 at 197.568 kb on - strand, within GFF888 at 197.568 kb on - strand, within GFF888 at 197.654 kb on + strand, within GFF888 at 197.654 kb on + strand, within GFF888 at 197.655 kb on - strand, within GFF888 at 197.841 kb on - strand, within GFF888 at 197.852 kb on + strand, within GFF888 at 197.867 kb on + strand, within GFF888 at 197.868 kb on - strand, within GFF888 at 197.960 kb on + strand, within GFF888 at 197.960 kb on + strand, within GFF888 at 197.960 kb on + strand, within GFF888 at 197.960 kb on + strand, within GFF888 at 197.961 kb on - strand, within GFF888 at 197.961 kb on - strand, within GFF888 at 198.078 kb on - strand at 198.078 kb on - strand at 198.078 kb on - strand at 198.307 kb on + strand at 198.307 kb on + strand at 198.308 kb on - strand at 198.308 kb on - strand at 198.368 kb on - strand, within GFF889 at 198.478 kb on + strand, within GFF889 at 198.478 kb on + strand, within GFF889 at 198.478 kb on + strand, within GFF889 at 198.478 kb on + strand, within GFF889 at 198.479 kb on - strand, within GFF889 at 198.479 kb on - strand, within GFF889 at 198.479 kb on - strand, within GFF889 at 198.479 kb on - strand, within GFF889 at 198.541 kb on + strand, within GFF889 at 198.601 kb on + strand, within GFF889 at 198.601 kb on + strand, within GFF889 at 198.602 kb on - strand, within GFF889 at 198.602 kb on - strand, within GFF889 at 198.712 kb on + strand, within GFF889 at 198.797 kb on - strand, within GFF889 at 198.797 kb on - strand, within GFF889 at 198.832 kb on + strand, within GFF889 at 198.833 kb on - strand, within GFF889 at 198.982 kb on + strand, within GFF889 at 198.989 kb on - strand, within GFF889 at 198.989 kb on - strand, within GFF889 at 198.989 kb on - strand, within GFF889 at 198.989 kb on - strand, within GFF889 at 198.989 kb on - strand, within GFF889 at 199.000 kb on + strand, within GFF889
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 196,136 + GFF886 0.60 +0.1 196,271 + GFF886 0.70 +2.1 196,271 + GFF886 0.70 -0.3 196,271 + GFF886 0.70 -0.6 196,271 + GFF886 0.70 -2.0 196,271 + GFF886 0.70 -0.1 196,271 + GFF886 0.70 -0.8 196,272 - GFF886 0.70 +0.1 196,272 - GFF886 0.70 +1.8 196,326 - GFF886 0.74 +1.1 196,565 + +1.0 196,565 + -0.1 196,565 + -0.4 196,886 + GFF887 0.51 -0.4 196,886 + GFF887 0.51 +0.8 196,886 + GFF887 0.51 -0.1 196,887 - GFF887 0.52 -1.5 196,887 - GFF887 0.52 -0.6 197,370 - GFF888 0.27 -0.8 197,567 + GFF888 0.47 -1.2 197,568 - GFF888 0.47 +0.4 197,568 - GFF888 0.47 +0.7 197,654 + GFF888 0.56 +0.2 197,654 + GFF888 0.56 +0.7 197,655 - GFF888 0.56 +0.5 197,841 - GFF888 0.75 -0.2 197,852 + GFF888 0.76 -0.3 197,867 + GFF888 0.78 +0.9 197,868 - GFF888 0.78 +1.1 197,960 + GFF888 0.88 +0.3 197,960 + GFF888 0.88 +0.5 197,960 + GFF888 0.88 -0.6 197,960 + GFF888 0.88 -0.5 197,961 - GFF888 0.88 -0.1 197,961 - GFF888 0.88 -0.4 198,078 - +0.6 198,078 - +0.2 198,078 - -0.6 198,307 + +0.2 198,307 + +0.4 198,308 - +0.5 198,308 - -0.6 198,368 - GFF889 0.11 +0.2 198,478 + GFF889 0.16 -0.7 198,478 + GFF889 0.16 +0.1 198,478 + GFF889 0.16 -1.4 198,478 + GFF889 0.16 +1.5 198,479 - GFF889 0.16 -0.6 198,479 - GFF889 0.16 +1.2 198,479 - GFF889 0.16 -1.6 198,479 - GFF889 0.16 -0.9 198,541 + GFF889 0.19 -0.6 198,601 + GFF889 0.22 +0.2 198,601 + GFF889 0.22 -0.7 198,602 - GFF889 0.22 -1.2 198,602 - GFF889 0.22 +1.0 198,712 + GFF889 0.27 +0.6 198,797 - GFF889 0.31 +0.6 198,797 - GFF889 0.31 +0.5 198,832 + GFF889 0.33 -0.6 198,833 - GFF889 0.33 -0.5 198,982 + GFF889 0.40 -0.1 198,989 - GFF889 0.40 -0.1 198,989 - GFF889 0.40 +0.2 198,989 - GFF889 0.40 -0.4 198,989 - GFF889 0.40 -0.2 198,989 - GFF889 0.40 +0.0 199,000 + GFF889 0.41 -0.5
Or see this region's nucleotide sequence