Strain Fitness in Variovorax sp. SCN45 around GFF7306

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7305 and GFF7306 are separated by 23 nucleotidesGFF7306 and GFF7307 are separated by 11 nucleotidesGFF7307 and GFF7308 are separated by 15 nucleotides GFF7305 - SAM-dependent methyltransferase, MidA, at 101 to 1,213 GFF7305 GFF7306 - FolM Alternative dihydrofolate reductase 1, at 1,237 to 2,013 GFF7306 GFF7307 - Dihydroneopterin aldolase (EC 4.1.2.25), at 2,025 to 2,423 GFF7307 GFF7308 - Uncharacterized MFS-type transporter, at 2,439 to 3,662 GFF7308 Position (kb) 1 2 3Strain fitness (log2 ratio) -2 -1 0 1 2at 1.187 kb on + strandat 1.358 kb on - strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.561 kb on + strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.562 kb on - strand, within GFF7306at 1.593 kb on + strand, within GFF7306at 1.593 kb on + strand, within GFF7306at 1.594 kb on - strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.667 kb on + strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.668 kb on - strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.717 kb on + strand, within GFF7306at 1.718 kb on - strand, within GFF7306at 1.718 kb on - strand, within GFF7306at 1.718 kb on - strand, within GFF7306at 1.718 kb on - strand, within GFF7306at 2.422 kb on - strandat 2.422 kb on - strandat 2.422 kb on - strandat 2.422 kb on - strandat 2.422 kb on - strandat 2.659 kb on + strand, within GFF7308at 2.659 kb on + strand, within GFF7308at 2.660 kb on - strand, within GFF7308

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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1,187 + -0.6
1,358 - GFF7306 0.16 +0.5
1,561 + GFF7306 0.42 +1.7
1,561 + GFF7306 0.42 +0.6
1,561 + GFF7306 0.42 +1.0
1,561 + GFF7306 0.42 +1.7
1,561 + GFF7306 0.42 +0.8
1,561 + GFF7306 0.42 +0.6
1,561 + GFF7306 0.42 +0.1
1,561 + GFF7306 0.42 +1.6
1,562 - GFF7306 0.42 +1.3
1,562 - GFF7306 0.42 +0.9
1,562 - GFF7306 0.42 +0.9
1,562 - GFF7306 0.42 +1.3
1,562 - GFF7306 0.42 +1.9
1,562 - GFF7306 0.42 +2.2
1,562 - GFF7306 0.42 +0.5
1,562 - GFF7306 0.42 +1.0
1,562 - GFF7306 0.42 +1.3
1,562 - GFF7306 0.42 +0.0
1,562 - GFF7306 0.42 +1.2
1,562 - GFF7306 0.42 +0.5
1,593 + GFF7306 0.46 +2.2
1,593 + GFF7306 0.46 +1.3
1,594 - GFF7306 0.46 +0.7
1,667 + GFF7306 0.55 +1.5
1,667 + GFF7306 0.55 +0.7
1,667 + GFF7306 0.55 +1.2
1,667 + GFF7306 0.55 +1.0
1,667 + GFF7306 0.55 +0.8
1,667 + GFF7306 0.55 +0.7
1,668 - GFF7306 0.55 +0.8
1,668 - GFF7306 0.55 +0.9
1,668 - GFF7306 0.55 +1.0
1,668 - GFF7306 0.55 +0.6
1,668 - GFF7306 0.55 +1.2
1,668 - GFF7306 0.55 +1.0
1,668 - GFF7306 0.55 +1.1
1,668 - GFF7306 0.55 -1.2
1,717 + GFF7306 0.62 +0.8
1,717 + GFF7306 0.62 +0.9
1,717 + GFF7306 0.62 -0.7
1,717 + GFF7306 0.62 +1.3
1,717 + GFF7306 0.62 +0.3
1,717 + GFF7306 0.62 +1.4
1,718 - GFF7306 0.62 +1.8
1,718 - GFF7306 0.62 +1.7
1,718 - GFF7306 0.62 -0.8
1,718 - GFF7306 0.62 +0.7
2,422 - -0.3
2,422 - -0.2
2,422 - -2.2
2,422 - -0.7
2,422 - -2.3
2,659 + GFF7308 0.18 -0.3
2,659 + GFF7308 0.18 +0.3
2,660 - GFF7308 0.18 +0.1

Or see this region's nucleotide sequence