Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6826 and GFF6827 are separated by 33 nucleotides GFF6827 and GFF6828 are separated by 85 nucleotides GFF6828 and GFF6829 are separated by 27 nucleotides
GFF6826 - Transcriptional regulator, LysR family, at 11,767 to 12,666
GFF6826
GFF6827 - Glutamine amidotransferase, class I, at 12,700 to 13,440
GFF6827
GFF6828 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 13,526 to 14,164
GFF6828
GFF6829 - Glutathione S-transferase (EC 2.5.1.18), at 14,192 to 14,773
GFF6829
Position (kb) 12
13
14 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 11.717 kb on + strand at 11.723 kb on + strand at 11.725 kb on + strand at 11.725 kb on + strand at 11.726 kb on - strand at 11.726 kb on - strand at 11.726 kb on - strand at 11.734 kb on + strand at 12.439 kb on + strand, within GFF6826 at 12.439 kb on + strand, within GFF6826 at 12.440 kb on - strand, within GFF6826 at 12.644 kb on - strand at 13.001 kb on - strand, within GFF6827 at 13.001 kb on - strand, within GFF6827 at 13.001 kb on - strand, within GFF6827 at 13.300 kb on + strand, within GFF6827 at 13.301 kb on - strand, within GFF6827 at 13.301 kb on - strand, within GFF6827 at 13.457 kb on + strand at 13.457 kb on + strand at 13.458 kb on - strand at 13.458 kb on - strand at 13.483 kb on - strand at 13.880 kb on + strand, within GFF6828 at 13.880 kb on + strand, within GFF6828 at 13.881 kb on - strand, within GFF6828 at 13.916 kb on + strand, within GFF6828 at 13.916 kb on + strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.964 kb on + strand, within GFF6828 at 13.964 kb on + strand, within GFF6828 at 14.207 kb on + strand at 14.207 kb on + strand at 14.207 kb on + strand at 14.207 kb on + strand at 14.208 kb on - strand at 14.208 kb on - strand at 14.271 kb on - strand, within GFF6829 at 14.271 kb on - strand, within GFF6829 at 14.321 kb on + strand, within GFF6829 at 14.322 kb on - strand, within GFF6829 at 14.322 kb on - strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.406 kb on - strand, within GFF6829 at 14.406 kb on - strand, within GFF6829 at 14.406 kb on - strand, within GFF6829
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 11,717 + +0.0 11,723 + +0.2 11,725 + -0.1 11,725 + -1.0 11,726 - +0.9 11,726 - +0.3 11,726 - +1.4 11,734 + -0.1 12,439 + GFF6826 0.75 -0.5 12,439 + GFF6826 0.75 -1.3 12,440 - GFF6826 0.75 +1.2 12,644 - -0.3 13,001 - GFF6827 0.41 -0.5 13,001 - GFF6827 0.41 +0.0 13,001 - GFF6827 0.41 +0.3 13,300 + GFF6827 0.81 +0.7 13,301 - GFF6827 0.81 +0.6 13,301 - GFF6827 0.81 -0.5 13,457 + +0.6 13,457 + +0.2 13,458 - -0.1 13,458 - -0.4 13,483 - +0.9 13,880 + GFF6828 0.55 -1.0 13,880 + GFF6828 0.55 +0.6 13,881 - GFF6828 0.56 -1.6 13,916 + GFF6828 0.61 -1.9 13,916 + GFF6828 0.61 -0.9 13,917 - GFF6828 0.61 -0.1 13,917 - GFF6828 0.61 +0.1 13,917 - GFF6828 0.61 -1.3 13,917 - GFF6828 0.61 -0.4 13,959 + GFF6828 0.68 -2.3 13,959 + GFF6828 0.68 -0.2 13,959 + GFF6828 0.68 +0.1 13,959 + GFF6828 0.68 +1.2 13,959 + GFF6828 0.68 -0.1 13,959 + GFF6828 0.68 +0.7 13,960 - GFF6828 0.68 -1.0 13,960 - GFF6828 0.68 -0.1 13,960 - GFF6828 0.68 +0.1 13,960 - GFF6828 0.68 -1.2 13,960 - GFF6828 0.68 -1.2 13,960 - GFF6828 0.68 -0.8 13,964 + GFF6828 0.69 +0.4 13,964 + GFF6828 0.69 +0.2 14,207 + -0.9 14,207 + -0.3 14,207 + -0.6 14,207 + -0.8 14,208 - -0.6 14,208 - -0.1 14,271 - GFF6829 0.14 -0.8 14,271 - GFF6829 0.14 -0.4 14,321 + GFF6829 0.22 -0.2 14,322 - GFF6829 0.22 -0.3 14,322 - GFF6829 0.22 +0.0 14,405 + GFF6829 0.37 +0.2 14,405 + GFF6829 0.37 -0.8 14,405 + GFF6829 0.37 -0.5 14,405 + GFF6829 0.37 -0.9 14,405 + GFF6829 0.37 -0.2 14,405 + GFF6829 0.37 +0.9 14,405 + GFF6829 0.37 +0.1 14,405 + GFF6829 0.37 -1.6 14,405 + GFF6829 0.37 -0.0 14,405 + GFF6829 0.37 +3.8 14,405 + GFF6829 0.37 -0.9 14,405 + GFF6829 0.37 -0.0 14,405 + GFF6829 0.37 -0.8 14,406 - GFF6829 0.37 -0.3 14,406 - GFF6829 0.37 +1.2 14,406 - GFF6829 0.37 +1.1
Or see this region's nucleotide sequence