Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6797 and GFF6798 overlap by 17 nucleotides GFF6798 and GFF6799 are separated by 27 nucleotides GFF6799 and GFF6800 are separated by 15 nucleotides GFF6800 and GFF6801 overlap by 4 nucleotides
GFF6797 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 44,945 to 45,730
GFF6797
GFF6798 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 45,714 to 46,367
GFF6798
GFF6799 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 46,395 to 47,057
GFF6799
GFF6800 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 47,073 to 47,876
GFF6800
GFF6801 - Transcriptional regulator, GntR family, at 47,873 to 48,664
GFF6801
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 45.643 kb on - strand, within GFF6797 at 45.643 kb on - strand, within GFF6797 at 45.758 kb on - strand at 45.788 kb on - strand, within GFF6798 at 46.081 kb on + strand, within GFF6798 at 46.082 kb on - strand, within GFF6798 at 46.082 kb on - strand, within GFF6798 at 46.201 kb on + strand, within GFF6798 at 46.201 kb on + strand, within GFF6798 at 46.201 kb on + strand, within GFF6798 at 46.202 kb on - strand, within GFF6798 at 46.202 kb on - strand, within GFF6798 at 46.202 kb on - strand, within GFF6798 at 46.202 kb on - strand, within GFF6798 at 46.382 kb on + strand at 46.382 kb on + strand at 46.382 kb on + strand at 46.383 kb on - strand at 46.383 kb on - strand at 46.383 kb on - strand at 46.465 kb on + strand, within GFF6799 at 46.465 kb on + strand, within GFF6799 at 46.466 kb on - strand, within GFF6799 at 46.535 kb on - strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 47.047 kb on + strand at 47.365 kb on + strand, within GFF6800 at 47.365 kb on + strand, within GFF6800 at 47.365 kb on + strand, within GFF6800 at 47.485 kb on + strand, within GFF6800 at 47.485 kb on + strand, within GFF6800 at 47.486 kb on - strand, within GFF6800 at 47.596 kb on + strand, within GFF6800 at 47.876 kb on + strand at 47.876 kb on + strand at 47.876 kb on + strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.893 kb on - strand at 47.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 45,643 - GFF6797 0.89 +0.3 45,643 - GFF6797 0.89 -1.3 45,758 - -0.7 45,788 - GFF6798 0.11 +3.0 46,081 + GFF6798 0.56 -1.6 46,082 - GFF6798 0.56 +1.1 46,082 - GFF6798 0.56 +0.2 46,201 + GFF6798 0.74 +0.2 46,201 + GFF6798 0.74 +1.2 46,201 + GFF6798 0.74 -1.6 46,202 - GFF6798 0.75 +0.3 46,202 - GFF6798 0.75 -0.6 46,202 - GFF6798 0.75 +1.0 46,202 - GFF6798 0.75 +1.0 46,382 + -0.7 46,382 + -0.3 46,382 + -1.7 46,383 - +0.5 46,383 - -0.3 46,383 - -0.9 46,465 + GFF6799 0.11 +0.1 46,465 + GFF6799 0.11 -0.2 46,466 - GFF6799 0.11 -0.0 46,535 - GFF6799 0.21 +0.1 46,879 + GFF6799 0.73 -0.2 46,879 + GFF6799 0.73 -0.2 46,879 + GFF6799 0.73 +0.0 46,879 + GFF6799 0.73 +0.7 46,879 + GFF6799 0.73 +0.5 46,879 + GFF6799 0.73 -0.2 46,880 - GFF6799 0.73 -3.0 46,880 - GFF6799 0.73 +0.1 46,880 - GFF6799 0.73 -0.6 46,880 - GFF6799 0.73 -1.8 46,880 - GFF6799 0.73 +0.7 46,880 - GFF6799 0.73 +0.1 47,047 + -0.4 47,365 + GFF6800 0.36 -0.8 47,365 + GFF6800 0.36 +1.3 47,365 + GFF6800 0.36 +0.0 47,485 + GFF6800 0.51 -0.4 47,485 + GFF6800 0.51 -0.4 47,486 - GFF6800 0.51 -0.8 47,596 + GFF6800 0.65 -0.6 47,876 + -0.4 47,876 + -0.0 47,876 + -0.4 47,877 - -0.1 47,877 - -0.8 47,877 - +0.5 47,877 - -0.1 47,877 - -1.5 47,877 - -0.7 47,877 - -0.3 47,877 - -1.0 47,877 - -0.4 47,877 - +0.5 47,877 - +0.0 47,877 - +0.7 47,888 + -0.7 47,888 + +0.1 47,888 + -0.6 47,888 + -0.2 47,889 - -0.1 47,889 - -0.4 47,889 - -0.0 47,889 - -0.6 47,889 - -0.6 47,889 - -0.0 47,892 + -0.6 47,892 + -0.3 47,892 + +0.4 47,892 + -0.1 47,893 - -0.7 47,893 - +0.3
Or see this region's nucleotide sequence