Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6730 and GFF6731 overlap by 8 nucleotides GFF6731 and GFF6732 overlap by 4 nucleotides GFF6732 and GFF6733 are separated by 4 nucleotides
GFF6730 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 40,752 to 41,504
GFF6730
GFF6731 - Nucleoside ABC transporter, permease protein 1, at 41,497 to 42,435
GFF6731
GFF6732 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 42,432 to 43,310
GFF6732
GFF6733 - hypothetical protein, at 43,315 to 44,517
GFF6733
Position (kb) 42
43
44 Strain fitness (log2 ratio) -1
0
1
2 at 41.567 kb on + strand at 41.610 kb on - strand, within GFF6731 at 41.610 kb on - strand, within GFF6731 at 41.765 kb on + strand, within GFF6731 at 41.766 kb on - strand, within GFF6731 at 41.766 kb on - strand, within GFF6731 at 41.978 kb on + strand, within GFF6731 at 41.978 kb on + strand, within GFF6731 at 41.979 kb on - strand, within GFF6731 at 42.175 kb on + strand, within GFF6731 at 42.175 kb on + strand, within GFF6731 at 42.176 kb on - strand, within GFF6731 at 42.221 kb on + strand, within GFF6731 at 42.221 kb on + strand, within GFF6731 at 42.222 kb on - strand, within GFF6731 at 42.222 kb on - strand, within GFF6731 at 42.311 kb on + strand, within GFF6731 at 42.312 kb on - strand, within GFF6731 at 42.490 kb on + strand at 42.490 kb on + strand at 42.491 kb on - strand at 42.523 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.632 kb on - strand, within GFF6732 at 42.632 kb on - strand, within GFF6732 at 42.662 kb on - strand, within GFF6732 at 43.219 kb on + strand, within GFF6732 at 43.253 kb on - strand at 43.253 kb on - strand at 43.453 kb on + strand, within GFF6733 at 43.453 kb on + strand, within GFF6733 at 43.453 kb on + strand, within GFF6733 at 43.454 kb on - strand, within GFF6733 at 43.616 kb on + strand, within GFF6733 at 43.700 kb on + strand, within GFF6733 at 43.701 kb on - strand, within GFF6733 at 43.701 kb on - strand, within GFF6733 at 43.736 kb on + strand, within GFF6733 at 43.737 kb on - strand, within GFF6733
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 41,567 + -1.3 41,610 - GFF6731 0.12 -0.9 41,610 - GFF6731 0.12 +1.1 41,765 + GFF6731 0.29 -0.2 41,766 - GFF6731 0.29 +0.1 41,766 - GFF6731 0.29 -0.5 41,978 + GFF6731 0.51 -0.8 41,978 + GFF6731 0.51 -0.7 41,979 - GFF6731 0.51 -0.7 42,175 + GFF6731 0.72 +0.1 42,175 + GFF6731 0.72 -0.1 42,176 - GFF6731 0.72 +0.1 42,221 + GFF6731 0.77 +0.0 42,221 + GFF6731 0.77 -0.4 42,222 - GFF6731 0.77 -0.9 42,222 - GFF6731 0.77 +1.5 42,311 + GFF6731 0.87 -0.2 42,312 - GFF6731 0.87 -0.4 42,490 + -0.2 42,490 + +0.6 42,491 - -0.0 42,523 + GFF6732 0.10 +0.0 42,526 + GFF6732 0.11 -0.2 42,526 + GFF6732 0.11 -0.6 42,526 + GFF6732 0.11 +0.0 42,526 + GFF6732 0.11 -0.2 42,526 + GFF6732 0.11 -0.7 42,527 - GFF6732 0.11 -0.7 42,527 - GFF6732 0.11 -1.4 42,527 - GFF6732 0.11 -0.6 42,527 - GFF6732 0.11 -0.5 42,632 - GFF6732 0.23 -0.8 42,632 - GFF6732 0.23 +0.5 42,662 - GFF6732 0.26 +0.6 43,219 + GFF6732 0.90 -0.8 43,253 - +0.4 43,253 - -0.1 43,453 + GFF6733 0.11 -0.4 43,453 + GFF6733 0.11 +0.5 43,453 + GFF6733 0.11 -1.5 43,454 - GFF6733 0.12 +0.3 43,616 + GFF6733 0.25 -0.0 43,700 + GFF6733 0.32 +2.0 43,701 - GFF6733 0.32 -0.2 43,701 - GFF6733 0.32 -0.5 43,736 + GFF6733 0.35 -0.2 43,737 - GFF6733 0.35 -0.7
Or see this region's nucleotide sequence