Strain Fitness in Variovorax sp. SCN45 around GFF6475

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6473 and GFF6474 are separated by 6 nucleotidesGFF6474 and GFF6475 are separated by 164 nucleotidesGFF6475 and GFF6476 are separated by 305 nucleotides GFF6473 - hypothetical protein, at 50,159 to 50,647 GFF6473 GFF6474 - hypothetical protein, at 50,654 to 51,073 GFF6474 GFF6475 - hypothetical protein, at 51,238 to 51,783 GFF6475 GFF6476 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 52,089 to 53,261 GFF6476 Position (kb) 51 52Strain fitness (log2 ratio) -2 -1 0 1 2at 50.378 kb on + strand, within GFF6473at 50.378 kb on + strand, within GFF6473at 50.379 kb on - strand, within GFF6473at 50.549 kb on + strand, within GFF6473at 50.549 kb on + strand, within GFF6473at 50.549 kb on + strand, within GFF6473at 50.550 kb on - strand, within GFF6473at 50.550 kb on - strand, within GFF6473at 50.550 kb on - strand, within GFF6473at 50.550 kb on - strand, within GFF6473at 50.836 kb on - strand, within GFF6474at 50.883 kb on + strand, within GFF6474at 50.883 kb on + strand, within GFF6474at 50.883 kb on + strand, within GFF6474at 50.883 kb on + strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.884 kb on - strand, within GFF6474at 50.925 kb on + strand, within GFF6474at 50.925 kb on + strand, within GFF6474at 50.925 kb on + strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.926 kb on - strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.928 kb on + strand, within GFF6474at 50.929 kb on - strand, within GFF6474at 50.929 kb on - strand, within GFF6474at 50.929 kb on - strand, within GFF6474at 51.102 kb on + strandat 51.102 kb on + strandat 51.102 kb on + strandat 51.102 kb on + strandat 51.103 kb on - strandat 51.103 kb on - strandat 51.103 kb on - strandat 51.103 kb on - strandat 51.162 kb on - strandat 51.421 kb on + strand, within GFF6475at 51.422 kb on - strand, within GFF6475at 51.565 kb on + strand, within GFF6475at 51.829 kb on + strandat 51.829 kb on + strandat 51.829 kb on + strandat 51.829 kb on + strandat 51.829 kb on + strandat 51.829 kb on + strandat 51.829 kb on + strandat 51.830 kb on - strandat 51.830 kb on - strandat 51.830 kb on - strandat 52.022 kb on + strandat 52.023 kb on - strandat 52.023 kb on - strandat 52.023 kb on - strandat 52.203 kb on + strandat 52.203 kb on + strandat 52.203 kb on + strandat 52.204 kb on - strandat 52.368 kb on + strand, within GFF6476at 52.675 kb on - strand, within GFF6476

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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50,378 + GFF6473 0.45 -0.2
50,378 + GFF6473 0.45 -1.1
50,379 - GFF6473 0.45 +0.4
50,549 + GFF6473 0.80 +0.8
50,549 + GFF6473 0.80 -0.4
50,549 + GFF6473 0.80 -0.5
50,550 - GFF6473 0.80 -0.2
50,550 - GFF6473 0.80 +0.1
50,550 - GFF6473 0.80 -0.2
50,550 - GFF6473 0.80 -0.6
50,836 - GFF6474 0.43 -0.2
50,883 + GFF6474 0.55 +0.3
50,883 + GFF6474 0.55 +0.1
50,883 + GFF6474 0.55 +0.1
50,883 + GFF6474 0.55 -0.2
50,884 - GFF6474 0.55 +0.6
50,884 - GFF6474 0.55 -0.3
50,884 - GFF6474 0.55 -0.3
50,884 - GFF6474 0.55 +0.5
50,884 - GFF6474 0.55 -1.4
50,884 - GFF6474 0.55 -0.1
50,884 - GFF6474 0.55 -1.6
50,925 + GFF6474 0.65 -1.0
50,925 + GFF6474 0.65 -1.1
50,925 + GFF6474 0.65 -0.7
50,926 - GFF6474 0.65 -1.1
50,926 - GFF6474 0.65 +0.1
50,926 - GFF6474 0.65 -0.3
50,926 - GFF6474 0.65 -1.5
50,926 - GFF6474 0.65 -0.1
50,926 - GFF6474 0.65 -0.6
50,928 + GFF6474 0.65 -0.2
50,928 + GFF6474 0.65 -0.2
50,928 + GFF6474 0.65 +0.0
50,928 + GFF6474 0.65 +0.1
50,928 + GFF6474 0.65 -0.9
50,928 + GFF6474 0.65 -0.0
50,929 - GFF6474 0.65 -0.3
50,929 - GFF6474 0.65 -0.3
50,929 - GFF6474 0.65 -1.1
51,102 + -0.9
51,102 + -0.3
51,102 + +2.1
51,102 + -0.3
51,103 - +0.3
51,103 - +0.4
51,103 - -0.1
51,103 - +0.4
51,162 - +0.5
51,421 + GFF6475 0.34 -2.0
51,422 - GFF6475 0.34 -0.8
51,565 + GFF6475 0.60 -1.1
51,829 + -0.2
51,829 + +0.2
51,829 + -0.9
51,829 + +0.7
51,829 + -0.9
51,829 + -0.7
51,829 + -0.4
51,830 - +0.1
51,830 - +2.1
51,830 - -0.5
52,022 + -1.4
52,023 - -0.4
52,023 - +0.1
52,023 - +0.5
52,203 + -1.3
52,203 + +0.3
52,203 + +1.0
52,204 - -0.4
52,368 + GFF6476 0.24 +0.5
52,675 - GFF6476 0.50 -1.0

Or see this region's nucleotide sequence