Strain Fitness in Variovorax sp. SCN45 around GFF6323

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
113,759	+		GFF6322	0.69	+0.4
113,759	+		GFF6322	0.69	+0.5
113,759	+		GFF6322	0.69	-0.2
113,759	+		GFF6322	0.69	+1.0
113,759	+		GFF6322	0.69	+0.7
113,759	+		GFF6322	0.69	-1.2
113,759	+		GFF6322	0.69	+1.1
113,759	+		GFF6322	0.69	-0.7
113,760	-		GFF6322	0.70	-0.0
113,760	-		GFF6322	0.70	-0.7
113,760	-		GFF6322	0.70	-0.3
113,760	-		GFF6322	0.70	-0.2
113,760	-		GFF6322	0.70	+0.2
113,760	-		GFF6322	0.70	-1.8
113,760	-		GFF6322	0.70	+0.7
113,760	-		GFF6322	0.70	+0.3
113,760	-		GFF6322	0.70	+0.6
113,760	-		GFF6322	0.70	-1.6
113,760	-		GFF6322	0.70	-0.5
113,760	-		GFF6322	0.70	+1.2
113,760	-		GFF6322	0.70	-0.5
113,760	-		GFF6322	0.70	+0.0
113,765	+		GFF6322	0.70	+0.2
113,765	+		GFF6322	0.70	+0.3
113,765	+		GFF6322	0.70	+0.5
113,765	+		GFF6322	0.70	+0.2
113,765	+		GFF6322	0.70	-0.9
113,765	+		GFF6322	0.70	-0.2
113,765	+		GFF6322	0.70	+0.3
113,765	+		GFF6322	0.70	-1.6
113,765	+		GFF6322	0.70	-0.9
113,765	+		GFF6322	0.70	+1.1
113,765	+		GFF6322	0.70	+0.6
113,765	+		GFF6322	0.70	+0.1
113,765	+		GFF6322	0.70	-0.4
113,765	+		GFF6322	0.70	+0.1
113,765	+		GFF6322	0.70	-0.5
113,765	+		GFF6322	0.70	-0.8
113,765	+		GFF6322	0.70	+0.2
113,765	+		GFF6322	0.70	+0.1
113,765	+		GFF6322	0.70	-0.2
113,765	+		GFF6322	0.70	-0.1
113,765	+		GFF6322	0.70	+0.2
113,766	-		GFF6322	0.70	-1.7
113,766	-		GFF6322	0.70	-0.3
113,766	-		GFF6322	0.70	-0.6
113,766	-		GFF6322	0.70	-0.5
113,766	-		GFF6322	0.70	+0.6
113,766	-		GFF6322	0.70	-1.1
113,766	-		GFF6322	0.70	-0.4
113,766	-		GFF6322	0.70	-0.2
113,766	-		GFF6322	0.70	-0.1
113,766	-		GFF6322	0.70	+0.1
113,766	-		GFF6322	0.70	-0.4
113,766	-		GFF6322	0.70	+0.5
113,766	-		GFF6322	0.70	-0.4
113,766	-		GFF6322	0.70	-0.5
113,766	-		GFF6322	0.70	-0.5
113,849	+		GFF6322	0.79	-0.6
114,002	+				-0.6
114,002	+				+0.2
114,002	+				-0.1
114,002	+				-0.2
114,002	+				-0.3
114,002	+				-1.3
114,002	+				-0.1
114,002	+				-0.2
114,002	+				-1.5
114,002	+				-0.1
114,003	-				+1.1
114,003	-				+0.5
114,003	-				-0.1
114,003	-				+0.3
114,003	-				-0.6
114,003	-				+1.4
114,003	-				+0.5
114,003	-				-1.2
114,430	+		GFF6323	0.30	+1.0
114,430	+		GFF6323	0.30	-1.5
114,430	+		GFF6323	0.30	-0.1
114,430	+		GFF6323	0.30	-0.2
114,430	+		GFF6323	0.30	+0.7
114,430	+		GFF6323	0.30	-0.7
114,430	+		GFF6323	0.30	-0.0
114,430	+		GFF6323	0.30	-1.7
114,430	+		GFF6323	0.30	-0.0
114,431	-		GFF6323	0.30	-0.7
114,431	-		GFF6323	0.30	-0.1
114,431	-		GFF6323	0.30	-1.5
114,431	-		GFF6323	0.30	-0.2
114,431	-		GFF6323	0.30	-0.1
114,431	-		GFF6323	0.30	-1.3
114,431	-		GFF6323	0.30	+0.5
114,431	-		GFF6323	0.30	+1.3
114,460	+		GFF6323	0.32	-1.5
114,610	+		GFF6323	0.44	+1.3
114,663	+		GFF6323	0.48	-0.5
114,664	-		GFF6323	0.48	+0.2
114,664	-		GFF6323	0.48	+0.1
114,787	+		GFF6323	0.58	-0.1
114,787	+		GFF6323	0.58	+0.1
114,788	-		GFF6323	0.58	-0.4
114,788	-		GFF6323	0.58	-0.9
114,788	-		GFF6323	0.58	+0.6
114,788	-		GFF6323	0.58	-0.5
114,788	-		GFF6323	0.58	-0.6
114,823	+		GFF6323	0.60	+0.5
114,823	+		GFF6323	0.60	-0.5
114,823	+		GFF6323	0.60	-0.2
114,823	+		GFF6323	0.60	-0.4
114,824	-		GFF6323	0.60	+1.4
114,853	+		GFF6323	0.63	-0.6
114,853	+		GFF6323	0.63	-0.3
114,853	+		GFF6323	0.63	-0.9
114,853	+		GFF6323	0.63	+0.4
114,853	+		GFF6323	0.63	+1.0
114,853	+		GFF6323	0.63	-0.8
114,853	+		GFF6323	0.63	+0.1
114,853	+		GFF6323	0.63	+0.5
114,853	+		GFF6323	0.63	-0.5
114,853	+		GFF6323	0.63	+0.4
114,853	+		GFF6323	0.63	+0.7
114,853	+		GFF6323	0.63	-0.9
114,854	-		GFF6323	0.63	-0.3
114,854	-		GFF6323	0.63	+0.3
114,854	-		GFF6323	0.63	-0.2
114,854	-		GFF6323	0.63	-0.5
114,854	-		GFF6323	0.63	-0.5
114,854	-		GFF6323	0.63	-0.4
114,854	-		GFF6323	0.63	-0.6
114,854	-		GFF6323	0.63	+0.3
114,854	-		GFF6323	0.63	-0.3
115,064	-		GFF6323	0.79	-0.2
115,513	+				-0.8
115,513	+				-0.0
115,513	+				-1.9
115,513	+				-0.4
115,513	+				-0.8
115,513	+				-0.4
115,513	+				-0.5
115,513	+				-0.3
115,513	+				+0.4
115,513	+				-0.5
115,513	+				+1.3
115,513	+				-0.2
115,513	+				-0.9
115,513	+				-1.1
115,513	+				+0.7
115,513	+				+0.1
115,513	+				+1.1
115,513	+				-1.4
115,513	+				-0.1
115,513	+				+2.1
115,514	-				-0.5
115,514	-				-0.9
115,514	-				-0.7
115,514	-				+0.0
115,514	-				-0.2
115,514	-				+0.2
115,514	-				-1.1
115,514	-				+0.2
115,514	-				-1.5
115,514	-				-0.9
115,600	+		GFF6324	0.13	-0.6
115,600	+		GFF6324	0.13	+0.4
115,600	+		GFF6324	0.13	+0.8
115,600	+		GFF6324	0.13	+0.2
115,601	-		GFF6324	0.13	-0.5
115,853	+		GFF6324	0.26	-0.0
116,056	+		GFF6324	0.36	+0.3
116,056	+		GFF6324	0.36	-0.4
116,119	+		GFF6324	0.39	-0.0
116,119	+		GFF6324	0.39	+0.1
116,120	-		GFF6324	0.39	-0.1
116,120	-		GFF6324	0.39	+0.7
116,206	+		GFF6324	0.44	-1.2
116,206	+		GFF6324	0.44	-1.7
116,206	+		GFF6324	0.44	+0.8
116,206	+		GFF6324	0.44	+1.7
116,206	+		GFF6324	0.44	-1.5
116,206	+		GFF6324	0.44	+1.0
116,206	+		GFF6324	0.44	-0.5
116,206	+		GFF6324	0.44	-1.0
116,206	+		GFF6324	0.44	+0.4
116,206	+		GFF6324	0.44	-1.9
116,207	-		GFF6324	0.44	+0.5
116,207	-		GFF6324	0.44	+0.5
116,207	-		GFF6324	0.44	-0.3
116,207	-		GFF6324	0.44	+0.6
116,207	-		GFF6324	0.44	-0.3
116,207	-		GFF6324	0.44	-1.4
116,260	+		GFF6324	0.47	-0.1
116,260	+		GFF6324	0.47	-0.1
116,261	-		GFF6324	0.47	-0.2
116,261	-		GFF6324	0.47	-0.4