Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6236 and GFF6237 overlap by 1 nucleotides GFF6237 and GFF6238 overlap by 1 nucleotides
GFF6236 - VgrG protein, at 26,092 to 28,746
GFF6236
GFF6237 - hypothetical protein, at 28,746 to 29,813
GFF6237
GFF6238 - hypothetical protein, at 29,813 to 31,132
GFF6238
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 28.069 kb on - strand, within GFF6236 at 28.447 kb on + strand, within GFF6236 at 28.448 kb on - strand, within GFF6236 at 28.487 kb on - strand at 28.487 kb on - strand at 28.493 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.598 kb on - strand at 28.598 kb on - strand at 28.598 kb on - strand at 28.695 kb on - strand at 28.745 kb on - strand at 28.745 kb on - strand at 28.754 kb on - strand at 28.836 kb on + strand at 28.837 kb on - strand at 28.837 kb on - strand at 28.837 kb on - strand at 29.355 kb on + strand, within GFF6237 at 29.356 kb on - strand, within GFF6237 at 29.381 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.430 kb on + strand, within GFF6237 at 29.437 kb on - strand, within GFF6237 at 29.478 kb on + strand, within GFF6237 at 29.507 kb on + strand, within GFF6237 at 29.507 kb on + strand, within GFF6237 at 29.572 kb on - strand, within GFF6237 at 29.572 kb on - strand, within GFF6237 at 29.793 kb on - strand at 29.793 kb on - strand at 29.793 kb on - strand at 29.793 kb on - strand at 29.807 kb on + strand at 29.808 kb on - strand at 29.895 kb on - strand at 29.895 kb on - strand at 29.930 kb on + strand at 29.931 kb on - strand at 29.931 kb on - strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.942 kb on + strand at 29.943 kb on - strand at 29.943 kb on - strand at 29.948 kb on + strand, within GFF6238 at 29.948 kb on + strand, within GFF6238 at 29.948 kb on + strand, within GFF6238 at 29.949 kb on - strand, within GFF6238 at 29.966 kb on + strand, within GFF6238 at 29.966 kb on + strand, within GFF6238 at 29.967 kb on - strand, within GFF6238 at 29.967 kb on - strand, within GFF6238 at 29.967 kb on - strand, within GFF6238 at 30.144 kb on - strand, within GFF6238 at 30.146 kb on + strand, within GFF6238 at 30.189 kb on + strand, within GFF6238 at 30.197 kb on + strand, within GFF6238 at 30.197 kb on + strand, within GFF6238 at 30.198 kb on - strand, within GFF6238 at 30.233 kb on + strand, within GFF6238 at 30.234 kb on - strand, within GFF6238 at 30.234 kb on - strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.344 kb on + strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.345 kb on - strand, within GFF6238 at 30.353 kb on + strand, within GFF6238 at 30.353 kb on + strand, within GFF6238 at 30.392 kb on + strand, within GFF6238 at 30.393 kb on - strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.417 kb on + strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.418 kb on - strand, within GFF6238 at 30.428 kb on + strand, within GFF6238 at 30.428 kb on + strand, within GFF6238 at 30.500 kb on + strand, within GFF6238 at 30.500 kb on + strand, within GFF6238 at 30.501 kb on - strand, within GFF6238
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 28,069 - GFF6236 0.74 +0.9 28,447 + GFF6236 0.89 +0.1 28,448 - GFF6236 0.89 -0.1 28,487 - -0.5 28,487 - -0.1 28,493 - +0.6 28,578 - +0.4 28,578 - -0.5 28,578 - +0.9 28,578 - -0.1 28,598 - +0.4 28,598 - -0.8 28,598 - -0.9 28,695 - -0.5 28,745 - -0.6 28,745 - +0.6 28,754 - -2.3 28,836 + +0.4 28,837 - +0.0 28,837 - -0.5 28,837 - +0.5 29,355 + GFF6237 0.57 +0.3 29,356 - GFF6237 0.57 +0.3 29,381 - GFF6237 0.59 +0.7 29,419 - GFF6237 0.63 -0.0 29,419 - GFF6237 0.63 +0.3 29,419 - GFF6237 0.63 -0.6 29,430 + GFF6237 0.64 -0.2 29,437 - GFF6237 0.65 +0.8 29,478 + GFF6237 0.69 -0.0 29,507 + GFF6237 0.71 +0.0 29,507 + GFF6237 0.71 -0.1 29,572 - GFF6237 0.77 +0.2 29,572 - GFF6237 0.77 +0.0 29,793 - -0.3 29,793 - -0.8 29,793 - -0.4 29,793 - -0.0 29,807 + +0.1 29,808 - -0.5 29,895 - -2.3 29,895 - +0.2 29,930 + -0.3 29,931 - -0.4 29,931 - +0.6 29,942 + -0.1 29,942 + -1.5 29,942 + -0.5 29,942 + +0.2 29,942 + -0.5 29,942 + -0.4 29,942 + -0.2 29,943 - +0.6 29,943 - -0.4 29,948 + GFF6238 0.10 -0.3 29,948 + GFF6238 0.10 +0.3 29,948 + GFF6238 0.10 -0.7 29,949 - GFF6238 0.10 +0.7 29,966 + GFF6238 0.12 -1.1 29,966 + GFF6238 0.12 -0.0 29,967 - GFF6238 0.12 +0.7 29,967 - GFF6238 0.12 -0.9 29,967 - GFF6238 0.12 +0.1 30,144 - GFF6238 0.25 -1.0 30,146 + GFF6238 0.25 -0.3 30,189 + GFF6238 0.28 -0.3 30,197 + GFF6238 0.29 -0.7 30,197 + GFF6238 0.29 -0.5 30,198 - GFF6238 0.29 +0.4 30,233 + GFF6238 0.32 -1.3 30,234 - GFF6238 0.32 +0.1 30,234 - GFF6238 0.32 +0.6 30,344 + GFF6238 0.40 +0.5 30,344 + GFF6238 0.40 -0.1 30,344 + GFF6238 0.40 +1.0 30,344 + GFF6238 0.40 +0.1 30,344 + GFF6238 0.40 -0.9 30,344 + GFF6238 0.40 -0.5 30,344 + GFF6238 0.40 -0.5 30,344 + GFF6238 0.40 -0.2 30,344 + GFF6238 0.40 +0.2 30,344 + GFF6238 0.40 +1.5 30,344 + GFF6238 0.40 -0.5 30,344 + GFF6238 0.40 -2.1 30,345 - GFF6238 0.40 -0.6 30,345 - GFF6238 0.40 -0.6 30,345 - GFF6238 0.40 -2.1 30,345 - GFF6238 0.40 -1.7 30,345 - GFF6238 0.40 -0.4 30,345 - GFF6238 0.40 -0.2 30,345 - GFF6238 0.40 -1.7 30,345 - GFF6238 0.40 +2.7 30,353 + GFF6238 0.41 -0.2 30,353 + GFF6238 0.41 -0.3 30,392 + GFF6238 0.44 -0.2 30,393 - GFF6238 0.44 +0.1 30,417 + GFF6238 0.46 +0.0 30,417 + GFF6238 0.46 -0.2 30,417 + GFF6238 0.46 -0.1 30,417 + GFF6238 0.46 +1.1 30,417 + GFF6238 0.46 -0.5 30,417 + GFF6238 0.46 +0.1 30,418 - GFF6238 0.46 +0.4 30,418 - GFF6238 0.46 +1.0 30,418 - GFF6238 0.46 -1.3 30,418 - GFF6238 0.46 +1.0 30,418 - GFF6238 0.46 +0.5 30,418 - GFF6238 0.46 +0.0 30,428 + GFF6238 0.47 +1.0 30,428 + GFF6238 0.47 +0.1 30,500 + GFF6238 0.52 -0.7 30,500 + GFF6238 0.52 -0.4 30,501 - GFF6238 0.52 +0.6
Or see this region's nucleotide sequence