Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6195 and GFF6196 are separated by 7 nucleotides GFF6196 and GFF6197 overlap by 4 nucleotides GFF6197 and GFF6198 are separated by 39 nucleotides
GFF6195 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 101,919 to 102,926
GFF6195
GFF6196 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 102,934 to 103,815
GFF6196
GFF6197 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 103,812 to 104,762
GFF6197
GFF6198 - D-malate dehydrogenase [decarboxylating] (EC 1.1.1.83), at 104,802 to 105,884
GFF6198
Position (kb) 103
104
105 Strain fitness (log2 ratio) -1
0
1
2 at 103.532 kb on - strand, within GFF6196 at 103.816 kb on - strand at 103.816 kb on - strand at 103.816 kb on - strand at 103.816 kb on - strand at 103.978 kb on + strand, within GFF6197 at 103.978 kb on + strand, within GFF6197 at 104.190 kb on + strand, within GFF6197 at 104.190 kb on + strand, within GFF6197 at 104.521 kb on + strand, within GFF6197 at 104.935 kb on + strand, within GFF6198 at 105.287 kb on - strand, within GFF6198 at 105.371 kb on - strand, within GFF6198 at 105.469 kb on + strand, within GFF6198 at 105.470 kb on - strand, within GFF6198 at 105.470 kb on - strand, within GFF6198 at 105.470 kb on - strand, within GFF6198 at 105.580 kb on + strand, within GFF6198 at 105.581 kb on - strand, within GFF6198 at 105.581 kb on - strand, within GFF6198 at 105.670 kb on + strand, within GFF6198 at 105.670 kb on + strand, within GFF6198 at 105.671 kb on - strand, within GFF6198 at 105.671 kb on - strand, within GFF6198 at 105.736 kb on + strand, within GFF6198 at 105.736 kb on + strand, within GFF6198 at 105.737 kb on - strand, within GFF6198 at 105.737 kb on - strand, within GFF6198
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 103,532 - GFF6196 0.68 +0.4 103,816 - -0.6 103,816 - +0.3 103,816 - -0.1 103,816 - +0.2 103,978 + GFF6197 0.17 +0.1 103,978 + GFF6197 0.17 +0.2 104,190 + GFF6197 0.40 +1.4 104,190 + GFF6197 0.40 +0.8 104,521 + GFF6197 0.75 +0.6 104,935 + GFF6198 0.12 -0.2 105,287 - GFF6198 0.45 -1.3 105,371 - GFF6198 0.53 +0.1 105,469 + GFF6198 0.62 +0.8 105,470 - GFF6198 0.62 +0.7 105,470 - GFF6198 0.62 +0.2 105,470 - GFF6198 0.62 +0.9 105,580 + GFF6198 0.72 +0.4 105,581 - GFF6198 0.72 +1.3 105,581 - GFF6198 0.72 +0.5 105,670 + GFF6198 0.80 -0.9 105,670 + GFF6198 0.80 +0.8 105,671 - GFF6198 0.80 +0.3 105,671 - GFF6198 0.80 +1.4 105,736 + GFF6198 0.86 +1.3 105,736 + GFF6198 0.86 +1.4 105,737 - GFF6198 0.86 +1.8 105,737 - GFF6198 0.86 +0.5
Or see this region's nucleotide sequence